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Learn how to visualize and quantify any gene, in any species, in any tissue down to the single-cell level with RNAscope® ISH. Request or register for a free seminar presented by RNascope® expert .

  • Jun
    01
    RNAscope® Technology Seminar - London, UK
    Francis Crick Institute
    RNAscope® Technology Seminar - London, UK
    Faraia Shah MPhil, PhD
    Sales Account Executive
    Ann-Marie Baker, PhD
    Postdoctoral Research Assistant
    Outline:

    Visualize Gene Expression & Genetic Variations in Tissues:

    Applications of RNAscope® and BaseScopeTM ISH technology

    RNAscope® is a proprietary RNA in situ hybridization (ISH) assay based on ACD patented signal amplification and background suppression technology which advances RNA biomarker analysis in tissues and cells. Unique to this technology, RNAscope® delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. This enables researchers to visualize which genes are expressed, localize where they are expressed, and quantify the level of expression.

    Key benefits

    • Detection of a single RNA molecule requires only three double Z probe pairs to bind to the target RNA molecule. The RNAscope® 20 double Z probe pairs design provides robustness against partial target RNA accessibility or degradation.
       
    • Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples. Rigorous double Z probes design eliminates cross hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology.
       
    • Provides cell-specific expression information in intact tissue architecture.
       
    • Works for virtually ANY gene from ANY species in ANY tissue.

    Presenters:

    Faraia Shah MPhil, PhD

    FShah@acdbio.com, Sales Account Executive, Advanced Cell Diagnostics

    Visualising gene expression and genetic variations in the tissue environment

     

    Ann-Marie Baker, PhD

    Postdoctoral Research Assistant, BART Cancer Institute

    'Investigating cancer evolution and heterogeneity using in situ hybridisation’

     

    Dates and Registration:
  • Jun
    02
    RNAscope® Technology Seminar - Oxford, UK
    MRC Weatherall Institute of Molecular Medicine (WIMM), University of Oxford, John Radcliffe Hospital, Oxford, UK
    RNAscope® Technology Seminar - Oxford, UK
    Matthew Nolan
    D.Phil candidate in clinical neuroscience at the University of Oxford
    Faraia Shah (MPhil, PhD)
    Sales Account Executive, Advanced Cell Diagnostics
    Outline:

    RNASCOPE® TECHNOLOGY 

    June 2, 2017

    12 - 1 pm

    MRC Weatherall Institute of Molecular Medicine (WIMM)

    University of Oxford

    John Radcliffe Hospital

    Oxford, UK


     

    The RNAscope® technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

     

    Matthew Nolan will present on the RNAscope® application in Neuroscience In-situ expression of post-mitotic transcription factors associated with projection neuron diversity in the adult post-mortem brain
     

     

    In addition Farai Shah will give an overview on:

    Visualizing gene expression and genetic variations in tissue environments of Neuroscience and Cancer related research by RNAscope® and BaseScope™

    • Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope® assay
    • Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
    • Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
    • Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system:
      • Co-expression with specific cell type markers
      • Sub-cellular localization of mRNA expression in cell body, axons or dendrites 
    • Distinguish specific homologues, paralogues, and orthologues by RNAscope® assay or BaseScope™ assay
    • Perform single cell analysis with morphological information in FFPE or frozen tissue
    • Pinpoint expression of novel RNAs of interest 
    Dates and Registration:
  • Jun
    07
    RNAscope® Technology Seminar - Bristol, UK
    University of Bristol, UK
    RNAscope® Technology Seminar - Bristol, UK
    Faraia Shah MPhil, PhD
    Sales Account Executive
    Phil Griffiths, PhD
    Research Associate
    Outline:

    Applications of RNAscope® and BaseScopeTM ISH technology
    The nervous system consists of numerous specialized cell types that remain to fully cataloged and characterized at the molecular level. Due to the high degree of structural and functional heterogeneity and the intricate spatial organization of these cells, it is of special importance to analyze gene expression in the presence of full morphological and spatial contexts. Due to the lack of specific antibody reagents, especially for lncRNAs, G-protein coupled receptors (GPCRs), and ion channels, mapping of specific transcripts by in situ hybridization offers an excellent alternative approach. The RNAscope® assay provides a powerful method to detect gene expression within the spatial and morphological tissue context. BaseScopeTM is a novel in situ hybridization technology that allows visualization of splice junctions between adjacent exons and/or retained introns in highly specific and sensitive manner, allowing characterization of alternative splicing and circular RNAs in cells and tissues.

    Faraia Shah, PhD will discuss how RNAscope® and BaseScopeTM assays, the two highly specific and sensitive ISH methods allow visualizing gene expression and genetic variations in tissue environments.  

    Phil Griffiths, PhD will present his own experience with RNAscope Assay applied to detect the gene expression of GPCRs and their ligands
     

     

    Dates and Registration:
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