Aperio RNA ISH Algorithm | In Situ Hybridization, RNA-ISH

Aperio RNA ISH Algorithm by Leica Biosystems

Original tissue showing RNA ISH detection single-plex with brown chromagen and hematoxylin nuclear counterstain. Mask shows Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Brown spot = Orange mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection single-plex with red chromagen and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection duplex red / green chromogens and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where tissue area that have no cells, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask, Green spot = Green mask. Pink annotations indicate the detection of RNA signal clusters.

Manual RNA ISH interpretation is time-consuming, subject to inter/intra-observer variability and typically employs semi-quantitative reads. The Aperio RNA ISH Algorithm from Leica Biosystems enables accurate counting of individual signals across the tissue, providing standardized, reproducible results. Validation testing has demonstrated the high correlation between the Aperio RNA ISH Algorithm and expert manual review, making it ideal for researchers who want to automate routine counting tasks.

Aperio RNA ISH Algorithm Features:

Quantify Single or Duplex signals
Identify subcellular location of signal (nucleus, cytoplasm)
Count individual cells or clusters
Quantitative and semi-quantitative outputs
Compatible with full Aperio Digital Pathology solution

Aperio RNA ISH Algorithm is compatible with the chromogenic RNAscope Manual and Automated Assays:

RNAscope™ 2.0 HD Reagent Kit—BROWN
RNAscope™ 2.0 HD Reagent Kit—RED
RNAscope™ 2.5 HD Reagent Kit—BROWN
RNAscope™ 2.5 HD Reagent Kit—RED
RNAscope™ 2-Plex Reagent Kit
RNAscope™ LS Reagent Kit—BROWN
RNAscope™ LS Reagent Kit—RED
RNAscope™ VS Reagent Kit—BROWN
RNAscope™ VS Reagent Kit—RED

The Aperio RNA ISH Algorithm forms part of Leica Biosystems’ comprehensive and open platform for ISH research, including the BOND RX rapid staining system, Aperio digital pathology scanners, and powerful software for image management and analysis. For more information, visit LeicaBiosystems.com/Aperio.

Aperio RNA ISH Algorithm and Leica Biosystems BOND RX are: For Research Use Only. Not For Use In Diagnostic Procedures.

LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH. Aperio and BOND are trademarks of the Leica Biosystems group of companies in the USA and optionally in other countries. Other logos, product and/or company names might be trademarks of their respective owners. 95.14542 Rev A 12/2016

For more information contact us at LeicaBiosystems.com.