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2021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease Applications
Anushka Dikshit, Ph.D.
Applications Scientist, Genomics, Advanced Cell Diagnostics
Watch Video:
Outline:

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot visualizing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples. The RNAscope™ technology provides pivotal single-cell information that is necessary to study novel cell populations in vital organs such as the liver and kidneys. Studying gene expression with spatial resolution can provide functional information for cellular sub-populations in turn improving our understanding of various disease pathologies.

Learning Objectives:
  • Understand complex spatial relationship among different cell types within the liver and kidneys.
  • Identify and characterize novel cell types .
  • Study expression of target genes implicated is liver and kidney diseases.
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