RNAscope Multiplex Fluorescent assay is ideal for single-molecule detection of multiple RNA targets simultaneously within the same tissue. The assay is very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells. The co-detection assay will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples. This webinar will help users understand the RNAscope Multiplex Fluorescent manual V2 assay workflow and discuss the tools for getting started. We will also share tips and tricks to perform the RNAscope Multiplex assays successfully to obtain publication-quality in situ RNA images.
- How the RNAscope assays work
- What you need to get started
- Tips and tricks for successful sample preparation
- Examples of staining results & research application specific data
- FAQs & Troubleshooting tips
