2019 Mar 26 - How to correctly interpret your RNAscope images
Connie Zhang, PhD.
Senior Technical Support Scientist
Watch Video:
Serdar Tulu, PhD.
Imaging Specialist
Amber Jolly, PhD.
Image Analysis Scientist
Outline:

RNAscope® assay is an innovative and proprietary RNA in situ hybridization (ISH) assay based on ACD’s patented technology with signal amplification and simultaneous background noise suppression. RNAscope® assay delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with anatomical context in a single assay. 

Download the Technote:

Tech Note for using ImageJ to analyze RNAscope® and BaseScope™ data

Who should attend:
  • Pathologists who may or may not be familiar with RNAscope® ISH
  • New and existing RNAscope® Assay users (manual and automated assay platforms)
  • Traditional ISH users new to the RNAscope® technology
  • IHC users interested in RNA ISH for complementary studies
Learning Objectives:

In this webinar, we will focus on how to review and analyze RNAscope® assay data images successfully. We will cover the following topics:

• Assess examples of RNAscope® images with poor, medium and high-quality target RNA staining

• Learn best practices for accurately interpreting your RNAscope® images and discuss RNAscope® scoring tips

• Review available types of data analysis and data presentation using HALOTM and open-source software on RNAscope® images

• Learn how to best capture high quality images for subsequent data analysis

• Review the current workflow of quantitative data analysis by the Pharma Assay Services Team

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