Point mutations result from changes in a single nucleotide base that may result in altered gene and protein function. Point mutations are especially important when the alterations occur in target genes implicated in pathological conditions. Tools such as PCR, sequencing and Northern blot The BaseScope assay can now detect transcripts down to a single nucleotide change, allowing for the detection of point mutations in FFPE tissues with morphological context. This is particularly applicable for the field of oncology, where point mutations can create intratumor heterogeneity and lead to therapy resistance.

 

FIGURE 1: Visualization of the point mutation BRAF V600E.  The assay was performed on SK-Mel-28 cells (homozygous for the BRAF V600E mutation) and CHL1a cells (homozygous for V600 BRAF) prepared as an FFPE cell pellet using single double-Z point mutation probes targeting either the BRAF V600E mutated sequence or BRAF V600 WT sequence, respectively. Arrows indicate positive signal.

 

 

FIGURE 2: Visualization of the point mutation KRAS G12C.  The assay was performed on SW1573 cells (homozygous for the KRAS G12C mutation) and Hut78 cells (homozygous for KRAS G12) prepared as an FFPE cell pellet using single double-Z point mutation probes targeting either the KRAS G12C mutated sequence or KRAS G12 WT sequence, respectively. Arrows indicate positive signal.

 

Top publications using the BaseScope™ assay for point mutation detection:

 

 

 

 

Validated BaseScope probes for Point Mutation List

 

  • BA-Hs-KRAS-G12A
  • BA-Hs-KRAS-G12C
  • BA-Hs-KRAS-G12D
  • BA-Hs-KRAS-G12S
  • BA-Hs-KRAS-G12V
  • BA-Hs-KRAS-nt34WT
  • BA-Hs-KRAS-nt35WT
  • BA-Hs-EGFR-T790M
  • BA-Hs-EGFR-T790WT
  • BA-Hs-BRAF-V600E
  • BA-Hs-BRAF-V600WT
  • BA-Hs-PIK3CA-H1047R
  • BA-Hs-PIK3CA-H1047WT

 

 

 

Want to order a probe or don't find your gene of interest in this list? Contact your Account Manager 

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Bio-Techne

19 Barton Lane  
Abingdon Science Park
Abingdon
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420

 

 

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china.ACD@bio-techne.com
For general information: Leon.Li@bio-techne.com
Web: www.acdbio.com/cn

For general information: Info.ACD@bio-techne.com
For place an order: order.ACD@bio-techne.com
For product support: support.ACD@bio-techne.com
For career opportunities: hr.ACD@bio-techne.com