miRNAscope HD Assay Red

miRNAscope™ assay used to detect miR-214-3p in different species samples including the mouse brain, rat brain, human brain, and mouse liver. miR-214-3p was successfully detected in the mouse, rat, and human brain tissue samples and not in the negative control mouse liver tissue sample.

miRNAscope™ assay used to specifically detect miR-138-5p in Purkinje cells in cerebellum and hippocampus regions of the mouse brain.

miRNAscope™ HD assay used to detect the outer nuclear layer (ONL) specific microRNA-182 in FFPE mouse eye sample. Negligible microRNA-182 is also detected in the inner nuclear layer and the ganglion cell layer.

Introducing the highly specific and sensitive miRNAscope™ Assay to detect small RNAs with spatial and morphological context at single-cell resolution. Leveraging ACD’s RNAscope™ core technology, the miRNAscope assay is designed to enable applications for small RNAs including the detection of antisense oligonucleotides (ASOs), microRNAs (miRNAs), small interfering RNAs (siRNAs), and other smaller RNAs that are 17-50 bases.

• Detect and identify cellular subtypes

• Visualize gene regulation with morphological context

• Validate miRNA biomarkers in intact tissues

• Assess small RNA therapeutic delivery mechanism

• Evaluate biodistribution and efficacy of therapy

• Add a visual dimension to heterogeneous tissue biology and analysis

The miRNAscope™ HD Assay Workflow Requires the following:

miRNAscope™ Target Probes (Catalog or Made-to-Order S1 Probes, not compatible with the RNAscope™ or BaseScope™ Assays)
miRNAscope™ Positive Control Probe (Cat. No. 727871-S1)
miRNAscope™ Negative Control Probe (Cat. No. 727881-S1)
miRNAscope™ HD Reagent Kit - RED (Cat. No. 324500) (Includes Pretreatment Kit, Detection kit, and wash buffer)
ImmEdge Hydrophobic Barrier Pen (Cat. No. 310018)
HybEZ™ Hybridization System

If using miRNAscope™ HD Assay for the first time, our Intro Packs include the universal positive and negative control probes, the Reagent Kit, the control cell pellets (Human or Mouse), the ImmEdge hydrophobic barrier pen, and the Vectamount mounting media. Be sure to order your specific miRNAscope™ target probes separately.

miRNAscope™ Intro Pack HD Reagent Kit RED – Hsa (Cat. No. 324530)
miRNAscope™ Intro Pack HD Reagent Kit RED – Mmu (Cat. No. 324531)