2022 May 31 - Detecting small regulatory RNAs with single-molecule sensitivity and single-cell resolution with the miRNAscope™ Assay
Eliot T Smith, PhD
Technical Support Scientist, Advanced Cell Diagnostics
Watch Video:
Outline:
 
Detecting small regulatory RNAs with single-molecule sensitivity and single-cell resolution with the miRNAscope™ Assay
The miRNAscope Assay uses a novel and proprietary in situ hybridization (ISH) technology to visualize spatial distributions of single RNA molecules 17-50 nt in length including microRNAs (miRNAs), short interfering RNAs (siRNAs), PIWI-interacting RNAs (piRNAs), and antisense oligonucleotides (ASOs). 
This webinar will provide an overview of the miRNAscope assay workflow, along with a summary of the miRNAscope technology as well as guidance for sample preparation and pretreatment, assay design, and troubleshooting.

 

 
 
Learning Objectives:
  • Overview of the RNAscope technology
  • Illustration of miRNAscope assay workflow and applications
  • Guidelines on sample preparation and pretreatment
  • Guidance on assay setup and appropriate controls
  • Troubleshooting Tips
  • Examples of miRNAscope results
 
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