BaseScope™ VS Reagent Kit – RED

The BaseScope™ VS Reagent Kit is designed to be automated on the DISCOVERY ULTRA Automated IHC/ISH slide staining systems from Roche Tissue Diagnostics. The BaseScope™ Assay leverages the same platform as proven and established within the RNAscope® technology but expands the detection capabilities, enabling the detection of short RNA target sequences and exon junctions in cells and tissues with morphological context, providing new data dimensions and unique insight into biological mechanisms.

The BaseScope™ VS Reagent Kit, with its high contrast Fast Red dye, is the first choice in in situ hybridization applications where chromogenic staining with DAB is less desirable (i.e. staining of highly pigmented lung, liver, retina and melanoma tissue specimens). Also, for genes where a lower expression is assumed, ACD recommends this kit, as the red dots clearly distinguish against the hematoxylin staining and visible under standard brightfield microscope.

Sample Images
BaseScope VS- Human Lung MET

BaseScope VS- Human Lung MET

BaseScope VS- Human Lung MET

BaseScope VS- Human Lung EGFR

Probe Compatibility

The BaseScope™ VS reagents require the new BaseScope™ VS Probes for optimal performance.

The BaseScope™ VS Reagent Kit automated workflow requires the following from Roche Tissue Diagnostics:

CAT #Order CodeProduct Name and Description
771-75805271916001250 Test Option# 8
760-23407099037001mRNA RED Detection Kit
760-24808127166001mRNA Sample Prep Kit
760-23607095341001mRNA RED Probe Amplification Kit
960-761 — 960-785 250 Test Probe Dispenser User-Fillable 1-25

The BaseScope™ VS Reagent Kit automated workflow requires the following from ACD:

  • BaseScope™ VS Target Probes (Catalog or Made-to-Order VS Probes)
  • BaseScope™ VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • BaseScope™ VS Reagent Kit
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