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RNAscope® ISH Probes

We offer probes for >9,000 targets across >140 species. New probes can be designed and manufactured in 2 weeks. 

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RNAscope® ISH in Publications

Featured in over 500+ publications, RNAscope® in situ hybridization has been applied in a multitude of research areas.

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Upcoming September Conferences

NSH Annual Meeting

International Academy of Pathology & EU Society of Pathology Congress

World CDx Boston Annual Meeting

 

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Webinars

Steps for Success with RNAscope® 2.5 Assay on Ventana Discovery Systems

Training Webinar July 19

 

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PD-L1 expression and CD274 gene alteration in triple-negative breast cancer: implication for prognostic biomarker.

Abstract

PURPOSE:

To estimate the therapeutic potential of PD-L1 inhibition in breast cancer, we evaluated the prevalence and significance of PD-L1 protein expression with a validated antibody and CD274 gene alternation in a large cohort of triple negative breast cancer (TNBC) and correlated with clinicopathological data and patients overall survival.

METHODS:

Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development

An increasing amount of evidence indicates that developmental programs are tightly regulated by the complex interplay between signalingpathways, as well as transcriptional and epigenetic processes. Here, we have uncovered coordination between transcriptional and morphogen cues to specify Merkel cells, poorly understood skin cells that mediate light touch sensations. In murine dorsal skin, Merkel cells are part of touch domes, which are skin structures consisting of specialized keratinocytes, Merkel cells, and afferent neurons, and are located exclusively around primary hair follicles.

Responses to crizotinib in patients with ALK-positive lung adenocarcinoma who tested immunohistochemistry (IHC)-positive and fluorescence in situ hybridization (FISH)-negative.

Although the Ventana immunohistochemistry (IHC) platform for detecting anaplastic lymphoma kinase gene (ALK) (D5F3) expression was recently approved by the US Food and Drugs Administration (FDA), fluorescence in situ hybridization (FISH) is still the "gold-standard" method recommended by the US National Comprehensive Cancer Network (NCCN) guideline for NSCLC. We evaluated 6 ALK-positive lung adenocarcinoma patients who tested Ventana IHC-positive and FISH-negative and assessed their clinical responses to the ALK tyrosine kinase inhibitor (TKI) crizotinib.

SUPERVISOR – REAGENT MANUFACTURING
SUPERVISOR – REAGENT MANUFACTURING

Advanced Cell Diagnostics, Inc. (ACD) is a leader in the field of molecular pathology and tissue-based

diagnostics. Our core RNAscope® technology is the world’s first multiplex fluorescent and chromogenic in

situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single molecule

sensitivity. RNAscope® is an ideal platform for developing the next generation of tissue-based companion

diagnostics. We are committed to continued innovation to bridge the gap between basic research and clinical

medicine to fulfill the promise of personalized medicine.

POSITION DESCRIPTION

We seek a highly motivated and dynamic individual to join our Operations team as a Supervisor - Reagent

Manufacturing. An ideal candidate will be someone who is comfortable working in a fast-paced start-up

environment, but who also has an understanding in delivering quality products to the market place. This

person must be willing to learn new techniques quickly and be able to adapt to shifting priorities in a fast-

paced and dynamic environment. The responsibility of this position is to coordinate manufacturing activities

within their area of responsibility and be accountable for the proper training and development of team

members.

2016 July 20

Education Experience

  •  A B.S. or M.S. within molecular biology, biochemistry, cell biology or related field with at least 4 years of industry experience
  • Must have demonstrated good biochemical laboratory skills
  • Able to work in cross functional team environments
  • Able to generate results and perform work with minimal supervision
  • Previous experience with ERP System preferred
  • Experience in ISO 13485 and GMP environment a plus
  • Candidate must be a strong team player, self-motivated and willing to learn new techniques and concepts in a fast paced environment
  • Strong organizational skills and attention to detail
  • Excellent verbal and written communication skills
  • At least 1 year in a lead or supervisory role

Duties and Responsibilities

  • Ensure execution of manufacturing activities in strict accordance with manufacturing batch records,standard operating procedures, and ACD policies
  • Ensures adherence to ISO 13485 at all times and GMP standards where appropriate
  • Coordinates manufacturing activities with formulation and filling groups to ensure the manufacturing schedule is on time
  • Ensures accurate accounting of inventory in area of responsibility
  • Escalates manufacturing issues related to personnel, procedures, materials, process, equipment, etc to Manufacturing Management and/or appropriate functional area(s)
  • Oversees and evaluates the performance of subordinates; ensures operators are properly trained and training is documented and current
  • Responsible for the editing and accuracy of MPRs, work instructions and SOPs relevant to area of responsibility through the document control process
  • Assists in process scale-up to transition from pilot lots to full production-scale manufacturing
  • Ensures that equipment is monitored, maintained, and within calibration and that new equipment is processed according to ISO 13485 standards
  • Review of batch records in area of responsibility
  • Resolve NCPs in area of responsibility

Occasional Duties and Responsibilities

  • May participate in Process Changes and Planned Deviations
  • May participate in the manufacture and/or formulations activities to meet deadlines
  • May assist in transferring processes from development to manufacturing
MANUFACTURING TECHNICIAN – FINISH AND FILL
MANUFACTURING TECHNICIAN – FINISH AND FILL

Advanced Cell Diagnostics, Inc. (ACD) is a leader in the field of molecular pathology and tissue-based

diagnostics. Our core RNAscope® technology is the world’s first multiplex fluorescent and chromogenic in

situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single molecule

sensitivity. RNAscope® is an ideal platform for developing the next generation of tissue-based companion

diagnostics. We are committed to continued innovation to bridge the gap between basic research and clinical

medicine to fulfill the promise of personalized medicine.

 

We seek a highly motivated and energetic Manufacturing Technician to join our Operations team. An ideal

candidate will have some ISO and GMP experience, but be able to adapt to shifting priorities in a fast-paced

and growing start-up environment. This position will involve following established procedures and protocols

to fill, assemble and label sub-assemblies for kit components as well as building kits from sub-assemblies.

2016 July 20

Education Experience

  • B.S. in Biological Sciences or A.A Degree is required

Preferred Qualifications

  • Detail oriented, efficient and a team player
  • Able to multi-task and prioritize
  • Experience working in an ISO 13485
  • Previous manufacturing experience in biotech or pharmaceutical company is strongly desired
  • Excellent verbal and written communication skills

Duties and Responsibilities

  • Execute fill and finish job functions on a daily basis with minimal supervision
  • Assist in other areas of manufacturing deemed high priority such as special builds, bulk orders,
  • inventory counts, or making of general use buffers etc.
  • Participate in general laboratory maintenance functions such as general cleaning of facility, cleaningof lab ware, changing of mats that are needed for normal manufacturing to occur
  • Place orders and organize manufacturing consumables

 

PHYSICAL REQUIREMENTS

  • This position requires lifting up 50 lbs, standing for long periods of time and carrying out repetitive tasks on a daily basis.

Occasional Duties and Responsibilities

  • Perform daily calibrations on key equipment
  • Participate in inventory counts
  • Perform some equipment maintenance; de-frost Freezers and Refrigerators, monthly cleaning etc.
Probe Design Scientist
R&D

Advanced Cell Diagnostics, Inc. (ACD) is a leader in the field of molecular pathology and tissue-based diagnostics. Our core RNAscope® technology is the world’s first multiplex fluorescent and chromogenic in situ hybridization platform capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity. RNAscope® is an ideal platform for developing the next generation of tissue-based companion diagnostics. We are committed to continued innovation to bridge the gap between basic research and clinical medicine to fulfill the promise of personalized medicine.

 

To accommodate the increased volume of custom probe orders and support needs, we plan to hire another probe design scientist to join our  R & D Team.

 

2016 July 15

Education Experience

  • PhD or MS with 3+ years of research experience in molecular biology, bioinformatics or a closely related field

Preferred Qualifications

  • In-depth knowledge of genes and sequences and related public databases
  • Strong familiarity with commonly used biological databases and bioinformatics software tools
  • Experience in biological sequence analysis tasks such as database searches and multiple sequence alignment
  • Familiarity with relational databases and scripting languages preferred
  • Strong organizational and interpersonal skills
  • Strong written and verbal communication skills

Duties and Responsibilities

  • Design RNAscope probes for target sequences using proprietary software
  • Manage probes synthesis
  • Manage probe data for internal and external distributions
  • Provide technical support to customers and sales team on probe design

Dedifferentiated Schwann Cell Precursors Secreting Paracrine Factors Are Required for Regeneration of the Mammalian Digit Tip.

Adult mammals have lost multi-tissue regenerative capacity, except for the distal digit, which is able to regenerate via mechanisms that remain largely unknown. Here, we show that, after adult mouse distal digit removal, nerve-associated Schwann cell precursors (SCPs) dedifferentiate and secrete growth factors that promote expansion of the blastema and digit regeneration. When SCPs were dysregulated or ablated, mesenchymal precursor proliferation in the blastema was decreased and nail and bone regeneration were impaired. Transplantation of exogenous SCPs rescued these regeneration defects.

In vivo evidence for a limited role of proximal tubular Klotho in renal phosphate handling.

Klotho is a transmembrane protein expressed in the renal tubules where it acts as a permissive coreceptor for fibroblast growth factor 23 (FGF23). FGF23 signaling reduces the abundance of CYP27b1 and phosphate cotransporters NPT2a and NPT2c, leading to a decrease in 1,25(OH)2D3synthesis and a rise in urinary phosphate excretion, respectively. Systemic or whole-nephron deletion of Klotho in mice results in renal FGF23 resistance characterized by high 1,25(OH)2D3 and phosphate levels and premature aging.

Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation.

The E3 ubiquitin ligase Mule is often overexpressed in human colorectal cancers, but its role in gut tumorigenesis is unknown. Here, we show in vivo that Mule controls murine intestinal stem and progenitor cell proliferation by modulating Wnt signaling via c-Myc. Mule also regulates protein levels of the receptor tyrosine kinase EphB3 by targeting it for proteasomal and lysosomal degradation. In the intestine, EphB/ephrinB interactions position cells along the crypt-villus axis and compartmentalize incipient colorectal tumors.

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Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.
Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com

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