In Situ Hybridization, RNA-ISH

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RNA expression
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Revolutionary RNAscope^® and BaseScope™ Assays
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Specific
Amplifies signal and simultaneously suppress noise
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Sensitive
Detects single RNA molecules
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Context
Detects RNA within conserved tissue or cell morphology
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Detect, localize and quantify vector and therapeutic transgenes

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Visualize and quantify expression of multiple checkpoint and immune cell markers

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Assess drug safety with unmatched sensitivity and specificity for any target in any tissue for any species

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Species-specific gene expression profiling of patient-derived xenograft tumors

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Fig 7: Detection of G protein-coupled receptors (GPCRs) in mouse brain hippocampus using the RNAscope<sup>®</sup> Multiplex Fluorescent assay on Fresh Frozen tissue samples: Cnr1 (Green) and Drd1 (Red). Cells are counterstained with DAPI.

Fig 7: Detection of G protein-coupled receptors (GPCRs) in mouse brain hippocampus using the RNAscope^® Multiplex Fluorescent assay on Fresh Frozen tissue samples: Cnr1 (Green) and Drd1 (Red). Cells are counterstained with DAPI.

Fig 8: Fluorescent detection of ion channels. Detection of Asic1 (Green) and Kcnj3 (Red) in mouse brain hippocampus using the RNAscope<sup>®</sup> Multiplex Fluorescent assay on Fresh Frozen tissue samples.

Fig 8: Fluorescent detection of ion channels. Detection of Asic1 (Green) and Kcnj3 (Red) in mouse brain hippocampus using the RNAscope^® Multiplex Fluorescent assay on Fresh Frozen tissue samples.

Fig 9: Detection of transcription factor immediate early gene and activity marker Cfos (Green) and GPCR Chrm3 (Red) in mouse hippocampus using the RNAscope^® Multiplex Fluorescent assay on Fresh Frozen samples. Cells are counterstained with DAPI.

Fig 10: Detection of the effector immediate early gene and plasticity marker Arc (Green) and GPCR Drd1 (Red) in mouse brain striatum using the RNAscope^® Multiplex Fluorescent assay on Fresh Frozen tissue samples.

Fig 11: Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope<sup>®</sup>2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin.

Fig 11: Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope^®2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin.

Fig 12: Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope<sup>®</sup>2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin.

Fig 12: Detection of G protein-coupled receptors (GPCRs) in normal mouse brain hippocampus using RNAscope^®2.5 HD Duplex Chromogenic assay on FFPE tissue samples: Cnr1 (Red) and Drd1 (Green). Cells are counterstained with hematoxylin.

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