Sensory experience sculpts neural connectivity via cellular and molecular mechanisms that are still largely unknown. Through a combination of single-cell transcriptomics, structural analysis, and electrophysiology, Dr. Lucas Cheadle and colleagues identified the secreted cytokine TWEAK and its receptor Fn14 as important regulators of sensory-dependent synaptic remodeling. Hear from Cheadle about his use of multiplexed mRNA detection in situ with spatial resolution to reveal temporally coordinated, experience-dependent expression of TWEAK in microglia and Fn14 in excitatory neurons, suggesting that experience regulates circuit development by initiating complementary transcriptional programs in neuronal and non-neuronal cells. This webinar is sponsored by Advanced Cell Diagnostics.
Topics to be covered:
- Single-cell transcriptomics and in situ validation of sensory-driven gene expression
- Sensory experience-dependent refinement of neural circuits by transcriptional control in activated neurons
- Induction of TWEAK expression in microglia by sensory stimulation
