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Co-Detection of RNA and Protein

Figure 1: FOXP3 protein visualized with PPIB mRNA

Figure 1: FOXP3 protein visualized with PPIB mRNA

Figure 2: Ki67 protein visualized with PPOLAR2A,PPIB and UBC mRNA

Figure 2: Ki67 protein visualized with PPOLAR2A,PPIB and UBC mRNA

Figure 3: Detection of protein markers in combination with PPIB mRNA using the RNAscope Red/Brown Duplex assay

Figure 3: Detection of protein markers in combination with PPIB mRNA using the RNAscope Red/Brown Duplex assay

Figure 4: Detection of protein markers in combination with PPIB mRNA using the RNAscope Red/Green Duplex assay

Figure 4: Detection of protein markers in combination with PPIB mRNA using the RNAscope Red/Green Duplex assay

ACD’s new Co-detection  Assays will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

 

The new assay and reagents are compatible with the Integrated Co-detection workflow schematic below

Key Application enabled with this new assay and workflow :

  • Detect pathogens and host cell markers
  • Detect non-coding RNA Targets
  • Identify cellular source of secreted proteins
  • Enable detection of additional targets such as splice variants, highly homologous targets
  • Visualize cell margin and RNA targets
  • Correlate  RNA-Protein expression
  • Antibody validation
 

Ordering Information

RNA-Protein Co-detection Ancillary Kit Cat No. 323180                                                                                

  • Co-detection Blocker
  • Co-detection Antibody Diluent
  • Co-detection Target Retrieval Reagents (10X)

 

Products Compatible with this Ancillary Kit

  • RNAscope Multiplex Fluorescent v2 Assay
  • RNAscope 2.5 HD RED Assay
  • BaseScope v2 RED Assay
  • RNAscope 2.5 LS Assay – RED
  • BaseScope LS Red Assay
  • RNAscope LS Multiplex Fluorescent Assay

 

Flyer

Simultaneous visualization Of RNA and protein targets using the new Co-detection kit

Download Flyer

 

 
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