The BaseScope™ Reagent Kit v2-RED, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. The BaseScope Assay is used for the detection of short RNA targets (50-300 bases), highly homologous sequences, RNA mutations, and exon junctions for the detection of splice variants, circular RNA, and gene fusion. The BaseScope Assays do not require an RNase-free environment that is used for traditional RNA ISH.
Applications of the BaseScope RED Assay
| Exon Junctions/ Splice Variants | Short/Homologous Target Sequences | Point Mutations* |
|---|---|---|
| Exon junctions/splice variants | Short targets/highly homologous gene sequences (50-300nt) | Point mutation |
| Circular RNA (cirRNA) | T cell receptors (TCRs) and CDR sequences in T-cell clones | Short InDel |
| Gene fusion | pre-miRNA | Homologues |
| Gene knockout (KO) | Gene editing/CRISPR | * ACD Validated Point Mutations Only |
One major application for the BaseScope RED Assay is to identify highly homologous short target sequences. The BaseScope RED Assay can discern highly homologous MAGEA gene family in intact tissue with morphological context.
BaseScope Probes for MAGEA Gene Family Detection
The BaseScope Reagent Kit v2-RED with Fast Red dye offers high contrast and easy dot visualization.
The BaseScope Assay Workflow Requires:
BaseScope Target Probes (Catalog or Made-to-Order C1 Probes)
BaseScope Control Probes (Select positive control probes and negative control probes)
Control Slides (optional)
BaseScope Reagent Kit v2-Red (Universal Pretreatment Kit, Detection kit, and Wash Buffer included)
Brightfield microscope or multispectral fluorescent imaging system
HybEZ™ I or II Hybridization System
