2018 Aug 2 - Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
Omer Bayraktar, Ph.D.
Wellcome Sanger Institute
Watch Video:
Xiao-Jun Ma, Ph.D.
Chief Scientific Officer, Advanced Cell Diagnostics
Outline:

Sequencing of RNA (RNASeq) can provide a trove of useful information for quantification and characterization of the transcriptome. During bulk sequencing, however, spatial and cellular heterogeneity information is lost. Enter single-cell–level analysis, which can provide unprecedented detail on cellular diversity in development, health, and disease. This emerging area of spatial transcriptomics aims to map a single-cell–level molecular atlas of human tissues in situ. However, mapping entire tissue areas at single-cell quantitative resolution presents significant challenges for imaging and data analysis. In this webinar, efforts to develop large-scale in situ transcriptomics will be described, using the mouse brain as a model system for mapping the molecular diversity of neuronal and glial cell types. The experimental workflow includes automated tissue processing, imaging and analysis for large-scale multiplex mapping, and quantification of gene expression across brain regions in situ at single-cell resolution. The technology that makes this high-resolution mapping possible will be described and compared with other current methods.

 

Watch the on-demand recording of this webinar here

Learning Objectives:

During the webinar, the speakers will:

  • Describe the power and usefulness of in situ transcriptomics
  • Discuss the application of this technology to large-scale tissue mapping in the mouse brain
  • Explain how in situ transcriptomics (using RNAScope technology) can complement RNASeq and single-cell RNASeq
  • Answer audience questions during the live broadcast!

To learn more about products or technologies related to this webinar, go to http://rna.acdbio.com/transcriptomics.

This webinar will last for approximately 60 minutes.

X
Contact Us

Complete one of the three forms below and we will get back to you.

For Quote Requests, please provide more details in the Contact Sales form below

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Bio-Techne

19 Barton Lane  
Abingdon Science Park
Abingdon
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420

 

 

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china.ACD@bio-techne.com
For general information: Leon.Li@bio-techne.com
Web: www.acdbio.com/cn

For general information: Info.ACD@bio-techne.com
For place an order: order.ACD@bio-techne.com
For product support: support.ACD@bio-techne.com
For career opportunities: hr.ACD@bio-techne.com