Dual ISH and IHC

Human lung cancer with human PPIB positive control mRNA probe in red, CAM5.2 acidic cytokeratin immunohistochemical staining in green with DAPI as counterstain.

Human lung cancer with human PPIB positive control mRNA probe in red, CAM5.2 acidic cytokeratin immunohistochemical staining in green with DAPI as counterstain.

Dual ISH-IHC - Detection of OLFM4 using RNAscope technology and Lyzozyme using IHC

Dual ISH-IHC - Detection of OLFM4 using RNAscope technology and Lyzozyme using IHC

Dual ISH-IHC. Detection of alpha-smooth muscle actin (SMA) using IHC (green) and Olfr558 using RNAscope technology (red)

Dual ISH-IHC. Detection of alpha-smooth muscle actin (SMA) using IHC (green) and Olfr558 using RNAscope technology (red)

Analysis of both RNA and protein provides invaluable information for understanding the regulation of expression. Both ISH and IHC technologies share common features that make them unique amongst the protein and RNA detection technologies.

    - Morphological context: detection of RNA and protein while conserving the cell and tissue structural integrity

    - Similar workflows from sample fixation, pre-treatment, hybridization, signal amplification and data analysis.

Due to the multiple benefits of running both ISH and IHC and the robustness of the RNAscope® assay, some customers developed a dual ISH-IHC protocol to combine both assays on the same sample slide. We conducted an interview with 4 researchers who developed their own dual ISH-IHC method to provide you some insights into their protocol.

Download the interview and learn from these experts how to develop a dual ISH-IHC protocol.

Interview with:

  • Kristie Wetzel, Novartis Institute for

    BioMedical Research, Cambridge, MA, USA.

  • Dr Bradley Spencer-Dene, The Francis Crick

    Institute, London, UK.

  • Wanda Wang, University of Pittsburgh, PA,

    USA.

  • Mike Millar, Queen’s Medical Research

    Institute, University of Edinburgh,

    Edinburgh, UK.

Recorded Webinar

Application of combined RNA in situ hybridization and multiplex immunohistochemistry to unravel tumor heterogeneity in prostate cancer.
Presented by Dr Nallasivam Palanisamy and Dr Courtney Anderson

Watch Webinar

Related Publications

Download our latest Dual ISH-IHC publication list with more than 30 publications.

Download Publication list

Related Protocol

Download our RNAscope® LS Multiplex Fluorescent Assay Combined with Immunofluorescence Technical Note.

Download Technical Note

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