NEW! Introducing our protease-free pretreatment workflow for sequential RNA-Protein detection

Analysis of both RNA and protein provides invaluable information for understanding the regulation of expression. Both ISH and IHC technologies share common features that make them unique amongst the protein and RNA detection technologies.

    - Morphological context: detection of RNA and protein while conserving the cell and tissue structural integrity

    - Similar workflows from sample fixation, pre-treatment, hybridization, signal amplification and data analysis.

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems and Roche Tissue Diagnostics, Inc to deliver RNAscope™ ISH automated assays. Both automated stainers offering the possibility to combine in situ hybridization and immunohistochemistry on the same slide.

Due to the multiple benefits of running both ISH and IHC and the robustness of the RNAscope assay, some customers developed a dual ISH-IHC protocol to combine both assays on the same sample slide. We conducted an interview with 4 researchers who developed their own dual ISH-IHC method to provide you some insights into their protocol.

Download the interview and learn from these experts how to develop a dual ISH-IHC protocol.