In this 60-min webinar, Dr. Ghislaine Lioux will present solutions to common RNAscopeTM image analysis challenges including how to optimize color deconvolution, nuclear segmentation, and how to manage spot clusters. A recommended end-to-end workflow, from selecting an imaging modality to validating image analysis results will be presented.
Join us for a live demonstration of HALO® image analysis on optimization of in situ hybridization (ISH) analysis, fluorescence in situ hybridization (FISH) analysis, and protein/ nucleic acid co-detection assay.
- How to address common image analysis challenges in RNAscope assays
- How to optimize HALO image analysis settings for ISH, FISH, and codetection assays such as ISH-IHC and FISH-IF
- About the checkpoints for RNAscope quantitative image analysis success