RNAscope Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH, with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS. Our recent advances in RNAscope technology include the development of the BaseScope™ assay, which enables detection of any mRNA, short RNA targets and exon junctions. We will discuss the benefits of both RNAscope and BaseScope assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.
- New to RNAscope manual assay
- New to RNAscope ISH with IHC experience
- New to RNAscope ISH with traditional ISH experience
- New to fluorescence ISH with chromogenic ISH experience
- How RNAscope and BaseScope assays work
- What tools are needed for getting started
- Tips and tricks for sample preparation
- FAQs & Troubleshooting tips
- Where to find information
