Akt Signaling Pathway

Expression of INPP5D gene (SHIP) in human liver cancer TMA sample

Expression of INPP5D gene (SHIP) in human liver cancer TMA sample

Expression of HSP90 gene in human liver cancer TMA sample

Expression of HSP90 gene in human liver cancer TMA sample

Expression of WNK1 gene in human liver cancer TMA sample

Expression of WNK1 gene in human liver cancer TMA sample

Expression of CDC37 gene in human liver cancer TMA sample

Expression of CDC37 gene in human liver cancer TMA sample

Expression of SYK gene in human liver cancer TMA sample

Expression of SYK gene in human liver cancer TMA sample

Expression of BCR in Human Liver TMA sample

Expression of BCR in Human Liver TMA sample

Low expression of IKBKE gene (IKK) in human liver cancer TMA sample

Low expression of IKBKE gene (IKK) in human liver cancer TMA sample

Expression of NF-KappaB gene in human liver cancer TMA sample

Expression of NF-KappaB gene in human liver cancer TMA sample

AKT3 gene expressed in human liver cancer using RNAscope 2.0 Assay Brown

AKT3 gene expressed in human liver cancer using RNAscope 2.0 Assay Brown

Expression of JAK1 gene in human liver cancer TMA sample

Expression of JAK1 gene in human liver cancer TMA sample

AKT1 gene expressed in human liver cancer using RNAscope 2.0 Assay Brown

AKT1 gene expressed in human liver cancer using RNAscope 2.0 Assay Brown

Expression of PTK2 (FAK) in human liver cancer TMA sample

Expression of PTK2 (FAK) in human liver cancer TMA sample

Expression of ILK gene in human liver cancer TMA sample

Expression of ILK gene in human liver cancer TMA sample

Akt Signaling Pathway

 

Pathway information and image are provided courtesy of R&D Systems, a Bio-Techne Brand. Much more in-depth information about this pathway is available on their website Read more

Serine/threonine kinase Akt plays critical role in regulating diverse cellular functions including metabolism, growth, proliferation, survival, transcription and protein synthesis. There are three highly related isoforms of Akt (Akt1, Akt2 and Akt3), which share many substrates but isoform-specific Akt substrates have also been identified.

In cells, receptor tyrosin kinasese, integrins, B and T cell receptors, cytokine receptors, G-protein-coupled receptors and other stimuli induce phosphoinositide 3-kinase (PI3K) to produce phospha- tidylinositol (3,4,5) trisphosphates (PIP3) through activate factors such as Focal Adhesion Kinase (FAK), Integrin-Linked Kinase (ILK), B-Cell Receptor (BCR), Spleen Tyrosine Kinase (SKY) and Janus Kinase-1 (JAK1). PIP3 then harbors pleckstrin-homol- ogy (PH) domains of Akt. Akt is activated by Phosphoinositide-Dependent Kinase-1 (PDK1), mTORC2, Cell Division Cycle-37 (CDC37), Heat Shock Protein-90KD (HSP90) through phosphorylation, whereas Akt is dephosphorylated by tumor suppressor phosphatase and tensin homolog (PTEN) and SH2-Containing Inositol Phosphatase (SHIP).

Akt regulates cell growth through its effects on the TSC1/TSC2 complex and mTORC signaling. Akt contributes to cell proliferation through phosphorylation of the CDK inhibitors p21 and p27. Akt is a major mediator of cell survival through direct inhibition of pro-apoptotic signals generated by transcription factors like FoxO or inhibition of pro-apoptotic proteins like Bad. Akt activates I-KappaB Kinase (IKK), which ultimately leads to NF-KappaB activation and cell survival. In the presence of survival factors, Akt1 phosphorylates FKHRL1 (FOXO3), leading to the association of FKHRL1 with 14-3-3 proteins and its retention in the cytoplasm. WNK Lysine deficient Protein Kinase-1 (WNK1) is a physiologically relevant target of Insulin signaling through PI3K and Akt and functions as a negative regulator of Insulin-stimulated mitogenesis. Akt phosphorylates Yes-Associated Protein-1 (YAP), a transcription factor, which causes nuclear export and cytoplasmic localization. Akt also phosphorylates Androgen Receptor (AR) which causes a decrease in AR activity on the p21 promoter and cell cycle progression.

Publications

There are several publications where the Akt gene and RNAscope ISH are referenced.

Let's get started on your project.

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com