miRNAscope LS Reagent Kit Red | In Situ Hybridization, RNA-ISH

miRNAscope™ LS Reagent Kit Red

miRNAscope™ LS assay used to detect cartilage-specific miR-140-5p (top row) in mouse embryo cartilage tissues including the digits, somites, and ear tissues. Scramble negative control (bottom row) was used to determine specificity and background.

miRNAscope™ LS assay demonstrates robust and specific detection of miR-124-3p in the mouse embryo tissues. miR-1243p (left image) was detected predominately in the mouse brain, but absent in other tissues such as the lung or liver. Scramble negative control probe (right image) was used to demonstrate specificity of the assay.

miRNAscope™ LS assay was used to detect the siRNA#1 probe. Above images showed specific detection of the siRNA#1 probe in the siRNA#1 treated sample (middle image) and not in the negative (left image) nor siRNA#2 treated samples (right image) showing successful delivery of siRNA#1 therapy and specificity of the miRNAscope Assay.

miRNAscope™ + IHC detection of myeloid-specific miR-223-3p (red staining) and T-cell marker CD3 (green staining) in human cervical cancer tissue. The ISH-IHC staining shows mutually exclusive localization of these two targets

The miRNAscope LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The highly specific and sensitive miRNAscope Assay is designed to detect small RNAs with spatial and morphological context at single-cell resolution. Leveraging ACD’s RNAscope core technology, the miRNAscope assay is designed to enable applications for small RNAs including the detection of antisense oligonucleotides (ASOs), microRNAs (miRNAs), small interfering RNAs (siRNAs), and other smaller RNAs that are 17-50 bases.

Detect and identify cellular subtypes
Visualize gene regulation with morphological context
Validate miRNA biomarkers in intact tissues
Assess small RNA therapeutic delivery mechanism
Evaluate biodistribution and efficacy of therapy
Add a visual dimension to heterogeneous tissue biology and analysis

These new miRNAscope LS reagents require the new miRNAscope LS Probes for optimal performance. miRNAscope LS probes are not compatible with RNAscope LS or BaseScope LS reagents

Flyer

Sensitive assay for ASO and siRNA Detection

Download Flyer

The miRNAscope LS Automated Assay workflow requires the following from Leica Biosystems (LeicaBiosystems.com):

CAT #	Product Name and Description	Quantity
OPT9049	BOND Titration Kit	10 containers, 50 inserts
OP79193	BOND Open Containers (7 mL)	10 pack
OP309700	BOND Open Containers (30 mL)	10 pack
S21.4611	BOND Universal Covertiles™	160 pack
AR9961 & AR9640	BOND Epitope Retrieval Solution 1 & 2	1L (RTU) each
S21.1971	BOND Mixing Stations	6 vials
AR9222	BOND Dewax Solution	1L (RTU)
AR9590	BOND Wash Solution (10x concentrate)	1L
DS9390	BOND Polymer Refine Detection	1 kit
CS9100	BOND Aspirating Probe Cleaning System	1 system, 15 cleans

miRNAscope LS Automated Assay—RED Workflow Requires the following from ACD:

miRNAscope LS Target Probes (Catalog or Made-to-Order LS-S1 Probes)
miRNAscope LS Positive Control Probe (Cat. No. 727878-S1)
miRNAscope LS Negative Control Probe (Cat. No. 727888-S1)
miRNAscope LS Reagent Kit - RED (Cat. No. 324600) (Includes Pretreatment Kit, Detection kit, and wash buffer)

Search form