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miRNAscope LS Reagent Kit Red

miRNAscope™ LS assay used to detect cartilage-specific miR-140-5p (top row) in mouse embryo cartilage tissues including the digits, somites, and ear tissues. Scramble negative control (bottom row) was used to determine specificity and background.

miRNAscope™ LS assay used to detect cartilage-specific miR-140-5p (top row) in mouse embryo cartilage tissues including the digits, somites, and ear tissues. Scramble negative control (bottom row) was used to determine specificity and background.

miRNAscope™ LS assay demonstrates robust and specific detection of miR-124-3p in the mouse embryo tissues. miR-1243p (left image) was detected predominately in the mouse brain, but absent in other tissues such as the lung or liver. Scramble negative control probe (right image) was used to demonstrate specificity of the assay.

miRNAscope™ LS assay demonstrates robust and specific detection of miR-124-3p in the mouse embryo tissues. miR-1243p (left image) was detected predominately in the mouse brain, but absent in other tissues such as the lung or liver. Scramble negative control probe (right image) was used to demonstrate specificity of the assay.

miRNAscope™ LS assay was used to detect the siRNA#1 probe. Above images showed specific detection of the siRNA#1 probe in the siRNA#1 treated sample (middle image) and not in the negative (left image) nor siRNA#2 treated samples (right image) showing successful delivery of siRNA#1 therapy and specificity of the miRNAscope Assay.

miRNAscope™ LS assay was used to detect the siRNA#1 probe. Above images showed specific detection of the siRNA#1 probe in the siRNA#1 treated sample (middle image) and not in the negative (left image) nor siRNA#2 treated samples (right image) showing successful delivery of siRNA#1 therapy and specificity of the miRNAscope Assay.

miRNAscope™ + IHC detection of myeloid-specific miR-223-3p (red staining) and T-cell marker CD3 (green staining) in human cervical cancer tissue. The ISH-IHC staining shows mutually exclusive localization of these two targets

miRNAscope™ + IHC detection of myeloid-specific miR-223-3p (red staining) and T-cell marker CD3 (green staining) in human cervical cancer tissue. The ISH-IHC staining shows mutually exclusive localization of these two targets

The miRNAscope™ LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The highly specific and sensitive miRNAscope™ Assay is designed to detect small RNAs with spatial and morphological context at single-cell resolution.  Leveraging ACD’s RNAscope™ core technology, the miRNAscope assay is designed to enable applications for small RNAs including the detection of antisense oligonucleotides (ASOs), microRNAs (miRNAs), small interfering RNAs (siRNAs), and other smaller RNAs that are 17-50 bases.

 

  • Detect and identify cellular subtypes
  • Visualize gene regulation with morphological context
  • Validate miRNA biomarkers in intact tissues
  • Assess small RNA therapeutic delivery mechanism
  • Evaluate biodistribution and efficacy of therapy
  • Add a visual dimension to heterogeneous tissue biology and analysis 

These new miRNAscope™ LS reagents require the new miRNAscope™ LS Probes for optimal performance. miRNAscope™ LS probes are not compatible with RNAscope™ LS or BaseScope™ LS reagents

Flyer

Sensitive assay for ASO and siRNA Detection

Download Flyer

 

 

The miRNAscope™  LS Automated Assay workflow requires the following from Leica Biosystems (LeicaBiosystems.com):

 



CAT #

Product Name and Description

Quantity

OPT9049

BOND Titration Kit

10 containers, 50 inserts

OP79193

BOND Open Containers (7 mL)

10 pack

OP309700

BOND Open Containers (30 mL)

10 pack

S21.4611

BOND Universal Covertiles™

160 pack

AR9961 & AR9640

BOND Epitope Retrieval Solution 1 & 2

1L (RTU) each

S21.1971

BOND Mixing Stations

6 vials

AR9222

BOND Dewax Solution

1L (RTU)

AR9590

BOND Wash Solution (10x concentrate)

1L

DS9390

BOND Polymer Refine Detection

1 kit

CS9100

BOND Aspirating Probe Cleaning System

1 system, 15 cleans

 

 

 

miRNAscope™ LS Automated Assay—RED Workflow Requires the following from ACD:

 

  • miRNAscope™ LS Target Probes (Catalog or Made-to-Order LS-S1 Probes)
  • miRNAscope™ LS Positive Control Probe (Cat. No. 727878-S1)
  • miRNAscope™ LS Negative Control Probe (Cat. No. 727888-S1)
  • miRNAscope™ LS Reagent Kit - RED (Cat. No. 324600) (Includes Pretreatment Kit, Detection kit, and wash buffer)
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