Best-in-class RNA ISH for your automated systems of choice

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Ventana Medical Systems, Inc to deliver RNAscope ISH automated assays.

RNAscope® In Situ Hybridization Assay Workflow

  • Step 1:

    Permeabilize

    Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.

  • Step 2:

    Hybridize

    Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.

  • Step 3:

    Amplify

    RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.

  • Step 4:

    Visualize

    Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.

  • Step 5:

    Quantify

    Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software.

Which Automated Assay is right for me?

RNAscope® Ventana Universal HRP Assay

RNAscope® Ventana Universal AP Assay

RNAscope® Ventana Duplex Assay

BaseScope™ Ventana Assay - RED

Automated System

Ventana DISCOVERY ULTRA

Ventana DISCOVERY ULTRA

Ventana DISCOVERY ULTRA

Ventana DISCOVERY ULTRA

Detection Type

Chromogenic/ Fluorescent

Chromogenic

Chromogenic

Chromogenic

Chromogen Used

Diaminobenzidine (DAB), Purple, Teal/DCC, FAM, FITC, Red610, Rhodamine, Rhodamine 6G and Cy5,

Fast Red

Diaminobenzidine (DAB) & Fast Red Or Teal & Fast Red

Fast Red

Reaction/Staining Type

Singleplex

Singleplex

Singleplex, Duplex

Singleplex

Key Benefit

Robust, sensitive, permanent stain Most widely used RNAscope assay

Robust, sensitive, permanent stain.

Simultaneous in situ detection of two RNA targets

Specific detection of splice variants/exon junctions and short targets

Ideal for

High throughput Routine applications

High throughput Routine applications

Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor)

Detection of splice variants and exon junctions, short insertions/deletions, short targets of complex & highly homologous gene families

Novel Gene or Unknown Expression

Compatible

Compatible

Compatible

Compatible

Archival Specimens

Compatible

Compatible

Compatible

Compatible

Microscope Imaging

Standard bright field/ Fluorescent

Standard bright field

Standard bright field

Standard bright-field

Sample Type

FFPE

FFPE

FFPE

FFPE

Quantitation Method

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

Visual inspection
HALO® SOFTWARE
Any open-source image analysis software

# of tissue sections per kit

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

Target Sequence length required

300-1000bp

300-1000bp

300-1000bp

50-300bp

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