Best-in-class RNA ISH for your automated systems of choice
For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Roche Tissue Diagnostics, Inc to deliver RNAscope ISH automated assays.
To learn more about our automated spatial RNA & protein Co-Detection workflow on the Roche DISCOVERY ULTRA Platform watch the on-demand webinar below:
Which Automated Assay is right for me?
RNAscope™ VS Universal HRP Assay | RNAscope™ VS Universal AP Assay | RNAscope™ VS Duplex Assay | BaseScope™ VS Assay - RED | |
|---|---|---|---|---|
Automated System | Roche DISCOVERY ULTRA | Roche DISCOVERY ULTRA | Roche DISCOVERY ULTRA | Roche DISCOVERY ULTRA |
Detection Options | Chromogenic/ Fluorescent | Chromogenic | Chromogenic | Chromogenic |
Chromogen Used | Diaminobenzidine(DAB) | Fast Red | DAB & Fast Red | Fast Red |
Reaction/Staining Type | Singleplex | Singleplex | Singleplex, Duplex | Singleplex |
Key Benefit | Robust, sensitive, permanent stain Most widely used RNAscope assay | Robust, sensitive, permanent stain. | Simultaneous in situ detection of two RNA targets | Specific detection of splice variants/exon junctions and short targets |
Ideal for | High throughput Routine applications | High throughput Routine applications | Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor) | Detection of splice variants and exon junctions, short insertions/deletions, short targets of complex & highly homologous gene families |
Novel Gene or Unknown Expression | Compatible | Compatible | Compatible | Compatible |
Archival Specimens | Compatible | Compatible | Compatible | Compatible |
Microscope Imaging | Standard bright field/ Fluorescent | Standard bright field | Standard bright field | Standard bright-field |
Sample Type | FFPE | FFPE | FFPE | FFPE |
Quantitation Method | Visual inspection | Visual inspection | Visual inspection | Visual inspection |
# of tissue sections per kit | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) | 60 (20 mm x 20 mm) |
Target Sequence length required | 300-1000bp | 300-1000bp | 300-1000bp | 50-300bp |
