Best-in-class RNA ISH for your automated systems of choice

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Ventana Medical Systems, Inc to deliver RNAscope ISH automated assays.

RNAscope® 2.5 VS Reagent Kits are offered for an automated walk-away solution on the DISCOVERY ULTRA and DISCOVERY XT automated tissue staining systems by Ventana Medical Systems, Inc automating the Hybridize and Amplify steps of the RNAscope® Workflow.

RNAscope® Automated VS Assay

RNAscope® In Situ Hybridization Assay Workflow

  • Step 1:

    Permeabilize

    Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.

  • Step 2:

    Hybridize

    Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.

  • Step 3:

    Amplify

    RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.

  • Step 4:

    Visualize

    Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.

  • Step 5:

    Quantify

    Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.

Which RNAscope® Automated Assay is right for me?

Assays & Features

RNAscope® 2.5 Ventana Assay - BROWN/ RED

RNAscope® VS Universal HRP/ AP Reagent Kit

RNAscope® VS Duplex Reagent Kit

Automated System

Ventana DISCOVERY ULTRA Ventana DISCOVERY XT

Ventana DISCOVERY ULTRA

Ventana DISCOVERY ULTRA

Assay Type

Chromogenic

Chromogenic/ Fluorescent

Chromogenic

Dye Used

Diaminobenzidine (DAB)/ Fast Red

various

Diaminobenzidine (DAB) & Fast Red

Multiplexing

Singleplex

Singleplex

Duplex

Key Benefit

Robust, sensitive, permanent stain Most widely used RNAscope assay

Robust, sensitive, permanent stain.

Simultaneous in situ detection of two RNA species

Ideal for

High throughput Routine applications

High throughput Routine applications

Co-localization studies to map co-expression of two targets (e.g. secreted ligand and its receptor)

Novel Gene or Unkown Expression

+++++

+++++

+++++

Archival Specimens

+++++

+++++

++

Microscope Imaging System

Standard bright field

Standard bright field

Standard bright field

Sample Type

FFPE

FFPE

FFPE

Quantitation Method

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

Visual inspection HALO™ SOFTWARE RNAscope® SpotStudio™ Software automated quantitation

# of tissue sections per kit

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

60 (20 mm x 20 mm)

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