One assay for short sequence, exon junction and RNA mutation analysis

The BaseScope™ Assay is a unique product from ACD, based on the same platform of proven and established RNAscope® technology that has been cited in over 900 publications in just 5 years. This powerful assay provides new data dimensions and unique insight into biological mechanisms. The BaseScope™ Assay enables applications such as the detection of short RNA targets (50-300 bases), highly homologous sequences, RNA mutations and exon junctions for the analysis of splice variants, circular RNA, or gene fusions.

With ACD's continued commitment to provide researchers advanced capabilities of performing RNA ISH, the development of the BaseScope™ product provides a key benefit to detect biological events in situ using a single Z pair (i.e. RNA sequence spanning 50bp). The BaseScope™ assay is developed with advances in probe design, leveraging core concepts of the ZZ patented technology, and applies a novel amplification system that generates increased signal with simultaneous background noise suppression.

RNAscope® In Situ Hybridization Assay Workflow

  • Step 1:

    Permeabilize

    Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.

  • Step 2:

    Hybridize

    Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.

  • Step 3:

    Amplify

    RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.

  • Step 4:

    Visualize

    Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.

  • Step 5:

    Quantify

    Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.

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Advanced Cell Diagnostics

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