The RNAscope 2.5 LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The RNAscope 2.5 LS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH.
RNAscope 2.5 LS Reagent Kit—BROWN is a very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope 2.5 LS Reagent Kit—BROWN is designed to provide more intense Diaminobenzidine (DAB) staining—ideal when low copy target gene expression is anticipated (1–20 copies per cell). The stained slides are visualized with brightfield microscopes.
These new RNAscope 2.5 LS reagents require the new RNAscope 2.5 LS Probes for optimal performance. Probe designs are identical to previous version, however the RNAscope 2.5 LS probes are manufactured for optimal performance with RNAscope 2.5 LS reagent chemistry.
