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  • Jun
    28
    2021 June 28 - A new era of RNA detection: Exploring new technologies and prospects
    Boye Schnack Nielsen, Ph.D.,, Manager of Molecular Histology Bioneer, Hørsholm, Denmark.
    2021 June 28 - A new era of RNA detection: Exploring new technologies and prospects
    Boye Schnack Nielsen, Ph.D.,
    Manager of Molecular Histology Bioneer, Hørsholm, Denmark.
    Outline:

    In addition to commonly studied messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), multiple types of RNAs have become evident over recent decades, including microRNA (miRNA), long non-protein coding RNA (lncRNA) and circular RNA (circRNA). However, the functions of these RNA types within our cells are not yet fully understood.



    With the development of new in situ detection technologies, RNA in situ hybridization has now been enrolled in diagnostics applications, enabling the visualization of RNA localization in tissues and cells to better address their roles in the tissue microenvironment and in molecular pathways.



    Join this webinar, with Dr. Boye Schnack Nielsen, Manager of Molecular Histology at Bioneer, to learn more about:

    Learning Objectives:
    • Our understanding of different RNA types
    • RNA in situ hybridization
    • Combined RNA and protein staining
    Dates and Registration:
    June
    28
    8am PST
    11am EST
    5pm CEST
    Register Now
  • Jun
    29
    2021 June 29 - ACD Support Webinar - Probe Design Overview & Applications
    Yuanyuan Liu, Ph.D.,, Senior Scientist, Research & Development, Advanced Cell Diagnostics, a Bio-Techne brand
    2021 June 29 - ACD Support Webinar - Probe Design Overview & Applications
    Yuanyuan Liu, Ph.D.,
    Senior Scientist, Research & Development, Advanced Cell Diagnostics, a Bio-Techne brand
    Outline:

    Overview of the RNAscope/BaseScope probe design strategies to achieve highly specific and sensitive detection of a variety of RNA targets in situ, including but not limited to RNA, mRNA, pre-RNA, TCR/BCR, Knockout validation and splice variant.

     
    Learning Objectives:
    • Introduction to the RNAscope/BaseScope/miRNAscope/DNAscope design workflow. 
    • Featured applications of RNAscope/BaseScope/miRNAscope/DNAscope probes including detection of RNA, mRNA, pre-RNA, mature microRNA, ASO, siRNA, TCR/BCR, Knockout validation, splice variants, DNA copy number and structural variations. 
    • Frequently asked questions - Applications (FAQs).
    Dates and Registration:
    June
    29
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Jul
    21
    2021 July 21: A Highly Sensitive Chromogenic DNA in situ Hybridization Technology with Clinical and Diagnostic Potential
    Xiao-Jun Ma, Ph.D., Chief Scientific Officer, Advanced Cell Diagnostics, a Bio-Techne Brand
    Robert Monroe, MD, Ph.D.,, Chief Medical Officer, Advanced Cell Diagnostics, a Bio-Techne Brand
    2021 July 21: A Highly Sensitive Chromogenic DNA in situ Hybridization Technology with Clinical and Diagnostic Potential
    Xiao-Jun Ma, Ph.D.
    Chief Scientific Officer, Advanced Cell Diagnostics, a Bio-Techne Brand
    Robert Monroe, MD, Ph.D.,
    Chief Medical Officer, Advanced Cell Diagnostics, a Bio-Techne Brand
    Outline:

    Fluorescent in situ hybridization (ISH) is a common tool to detect and visualize specific DNA sequences in single cells. Current DNA ISH methods are inferior because they detect genes at low resolution due to long DNA probes and they require expensive, high resolution microscopes. This webinar will introduce DNAscopeTM, an innovative DNA ISH technology that employs a new probe design allowing for the precise targeting of small DNA stretches and convenient visualization under standard light microscopes.

    Learning Objectives:
    • How ‘double Z’ probe design promotes accurate target labeling
    • How to detect DNA breaks and copy number variations
    • The clinical potential of DNAscope for both pathology and diagnostic applications
    Dates and Registration:
    July
    21
    8am PST
    11am EST
    Register Now
  • Jul
    27
    2021 July 27: Introducing the RNAscope HiPlex v2.0 assay for single-cell and spatial transcriptomic profiling.
    Anushka Dikshit, Ph.D., Applications Scientist, Genomics, Advanced Cell Diagnostics
    Purvi Shah, Sr. Product Manager, Genomics, Advanced Cell Diagnostics
    2021 July 27: Introducing the RNAscope HiPlex v2.0 assay for single-cell and spatial transcriptomic profiling.
    Anushka Dikshit, Ph.D.
    Applications Scientist, Genomics, Advanced Cell Diagnostics
    Purvi Shah
    Sr. Product Manager, Genomics, Advanced Cell Diagnostics
    Outline:

    Introducing the RNAscope™ HiPlex v2.0 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples.

    The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex v2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex v2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME)

    Learning Objectives:
    • Learn about the expanded capabilities and applications of the HiPlex v2 assay
    • Understand how the assay enables characterization of immune cell types and activation states
    • Study complex spatial relationships among different cell types within the TME
    Dates and Registration:
    July
    27
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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