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  • Dec
    15
    2020 Dec 15: PAS Webinar: Use of RNAscope ISH services for T-Cell Therapy Detection & Quantification
    Christopher Bunker, Ph.D., MBA, Senior Director, Business Development, Advanced Cell Diagnostics
    2020 Dec 15: PAS Webinar: Use of RNAscope ISH services for T-Cell Therapy Detection & Quantification
    Christopher Bunker, Ph.D., MBA
    Senior Director, Business Development, Advanced Cell Diagnostics
    Outline:
    T Cell Therapy Detection and Quantification in Solid Tumors by RNAscope® in situ hybridization.
     
    RNAscope in situ hybridization (ISH) is the only method for specific detection of engineered CAR-T and TCR-T cell therapies in clinical trial patient biopsies.  IHC is unable to differentiate engineered T cells from endogenous cells. However, RNAscope probes targeting viral untranslated regions (UTRs) common to CAR and TCR mRNAs, enable specific detection and quantification of CAR/TCR-T cells in the tumor microenvironment (TME). RNAscope UTR probes are easily multiplexed with probes to assess activation, e.g., IFNG and GZMB, as well as with T cell IHC markers, e.g. CD3, CD4, CD8 to measure T cell recruitment. These assays are used to monitor T cell therapies in post-treatment patient biopsies and in preclinical animal models of safety and efficacy. 
     
     
    Learning Objectives:
    Key advantages of RNAscope cell therapy assays:
     
    • Simultaneously detect CAR/TCR-T cells with cytokines, immune cell markers and target antigens
    • Quantification of cell therapy tumor and stromal distribution and and T cell recruitment
    • Patient biopsy analysis to link cell therapy pharmacokinetics/pharmacodynamics and efficacy. 
    Dates and Registration:
    December
    15
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Dec
    17
    2020 Dec 17: ACD Support Webinar - Applications Summary for 2020
    Anushka Dikshit, Ph.D., Applications Scientist, Genomics, Advanced Cell Diagnostics
    2020 Dec 17: ACD Support Webinar - Applications Summary for 2020
    Anushka Dikshit, Ph.D.
    Applications Scientist, Genomics, Advanced Cell Diagnostics
    Outline:
    What did we learn from 2020? : Key publications utilizing RNAscope™ and BaseScope™ assay in diverse application areas
     
    The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot visualizing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples.  
     
    Join us to review some of the key publications from 2020 utilizing the RNAscope and BaseScope assays in a multitude of research areas, including neuroscience, oncology, cell and gene therapy,  infectious disease, single cell biology, and more.
     
    Learning Objectives:
    • Overview of the RNAscope technology and product line
    • Details on our newly launched miRNAscope assay, RNA-protein Co-detection kits and more
    • Key publications from 2020 using RNAscope and BaseScope assays in some established and novel application areas.
    Dates and Registration:
    December
    17
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

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