• Sep
    26
    2023 Sept 26 - Automate your Spatial RNA and Protein Detection with RNAscope ISH on the Roche DISCOVERY ULTRA
    Blake McAlpin, Ph.D.,, Field Applications Scientist, Advanced Cell Diagnostics, Bio-Techne.
    2023 Sept 26 - Automate your Spatial RNA and Protein Detection with RNAscope ISH on the Roche DISCOVERY ULTRA
    Blake McAlpin, Ph.D.,
    Field Applications Scientist, Advanced Cell Diagnostics, Bio-Techne.
    Outline:

     

    SAVE THE DATE - Join us for this co-sponsored webinar, where Blake McAlpin, Ph.D., will review how the RNAscope™ ISH workflow may be automated on the DISCOVERY ULTRA by Roche Tissue Diagnostics.

    Registration link coming soon.

    Automating RNAscope™ ISH capabilities in Spatial RNA and Protein Detection on the DISCOVERY ULTRA from Roche Tissue Diagnostics.

    RNAscope™ is the gold standard for RNA in situ hybridization, supported by over 8,000 peer reviewed publications and an extensive probe catalog of over 45,000 RNA probes.

    In this webinar, we demonstrate how RNAscope ISH technology can be automated on the DISCOVERY ULTRA platform from Roche Tissue Diagnostics, providing robust in situ hybridization assays including the expanding  visualization of spatial RNA and protein expression. 

     

    The live Q&A will be joined by

    Wendy French, Ph.D.,

    Senior Applications Support Principal

    Roche Tissue Diagnostics

     
    Dates and Registration:
    September
    26
    7am PST
    10am EST
    4pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

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Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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