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  • Apr
    15
    2021 Apr 15 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression Analysis
    Mansi Garg, M.S., Product Manager, Advanced Cell Diagnostics, a Bio-Techne brand
    Ruby Hsu, Ph.D.,, Director, Business Development Advanced Cell Diagnostics, a Bio-Techne brand
    2021 Apr 15 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression Analysis
    Mansi Garg, M.S.
    Product Manager, Advanced Cell Diagnostics, a Bio-Techne brand
    Ruby Hsu, Ph.D.,
    Director, Business Development Advanced Cell Diagnostics, a Bio-Techne brand
    Outline:
    This webinar will introduce our RNAscope in situ hybridization (ISH) services, as well as our quantitative image analysis capabilities and quality-controlled tissue bank for target validation.


    Bio-Techne’s Professional Assay Services (PAS) is the only CRO specialized in RNAscope, BaseScope™ and miRNAscope™ ISH services. We provide tissue-based and cell specific analysis of targets and biomarkers in as little as 4 weeks.



    Please join us to hear how our services are advancing the research and drug development at hundreds of academic and clinical institutions worldwide. Hear examples of studies in immuno-oncology, cell and gene therapy, neuroscience, single-cell RNAseq validation, biomarker validation, and more.
    Learning Objectives:
    • Visualize the cellular heterogeneity of tissues and solid tumors with the multiplexing and spatial capabilities of the RNAscope Multiplex Fluorescent assay
    • Specific and sensitive biomarker validation to accelerate translation of assays into the clinic
    • Morphology-based biodistribution analysis of AAV vector and transgene expression
    • CAR-T and TCR-T cell tumor infiltration and activity assays
    • Detection of splice variants, point mutations and short target sequences with BaseScope ISH assays
    • Co-stain with cell-type markers using our ISH/IHC Co-Detection workflow
    • PAS’s quality-controlled tissue bank including solid tumors, diseased and normal human tissues, and TMAs
    • RNAscope image analysis offerings for quantitative approaches to scoring
    Dates and Registration:
    April
    15
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Apr
    20
    2021 April 20/21 - ACD | Bio-Techne Genomics Oncology Summit
    Asmerom Sengal, MD, Ph.D.,, Postdoctoral Fellow Translational Research Institute (TRI), Australia
    Nallasivam Palanisamy, Ph.D., Associate Scientist/Professor; Department of Urology, Henry Ford Health System USA
    Lasse Sommer Kristensen, Ph.D.,, Associate Professor, Department of Biomedicine, Aarhus University, Denmark
    Anushka Dikshit, Ph.D.,, Applications Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
    2021 April 20/21 - ACD | Bio-Techne Genomics Oncology Summit
    Asmerom Sengal, MD, Ph.D.,
    Postdoctoral Fellow Translational Research Institute (TRI), Australia
    Nallasivam Palanisamy, Ph.D.
    Associate Scientist/Professor; Department of Urology, Henry Ford Health System USA
    Lasse Sommer Kristensen, Ph.D.,
    Associate Professor, Department of Biomedicine, Aarhus University, Denmark
    Anushka Dikshit, Ph.D.,
    Applications Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
    Outline:
    The RNAscope™ technology allows high sensitivity and spatial resolution providing pivotal single-cell information to gain better insights into tumor immunology, the complex and heterogeneous tumor microenvironment and its dynamics in cancer initiation, progression, and metastasis. Using the different assays under the RNAscope technology umbrella, it is possible to simultaneously visualize and assess the gene expression of cytokines, chemokines, immune cell markers, tumor cell markers and checkpoint markers within the tumor microenvironment (TME).


     
     
     
    Learning Objectives:

    Learn how experts in the field of Oncology have utilized the RNAscope, BaseScope™ and Co-Detection Workflow Assays to address pertinent questions in their research.

    The Bio-Techne Genomics Oncology Summit will be broadcast across three worldwide regions - Select your preferred Webinar Time in the registration form.

    Dates and Registration:
    April
    20
    10am PST
    10am SGT
    10am GMT
    Register Now
  • Apr
    27
    2021 April 27: Introducing Novel Chromogenic ISH Assay for High-Resolution Detection of DNA Copy Number and Structural Variants
    Madhura Nayak, Product Manager, Advanced Cell Diagnostics, a Bio-Techne Brand
    Vasudha Murlidhar, Ph.D., Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
    2021 April 27: Introducing Novel Chromogenic ISH Assay for High-Resolution Detection of DNA Copy Number and Structural Variants
    Madhura Nayak
    Product Manager, Advanced Cell Diagnostics, a Bio-Techne Brand
    Vasudha Murlidhar, Ph.D.
    Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
    Outline:

    Introducing ACD's new/ novel chromogenic in situ hybridization assay for high-resolution detection of DNA copy number and structural variations- DNAscope Assays.

    DNA ISH is a spatial profiling tool based on hybridizing labelled complementary probes to the target DNA inside the cell nucleus to visualize any genetic alterations in tissues. Learn how current limitations of the DNA ISH assay such as  limited morphological detail, difficult interpretation and low resolution detection due to larger probe sizes  are addressed by the new assay. The DNAscope chromogenic duplex (red/blue) staining allows researchers to use a standard bright-field microscope to visualize and quantify gene copy number variations (amplifications/deletions) and gene rearrangements/fusions in tissues with spatial and morphological context at single cell resolution.

    Learning Objectives:
    • Spatial Visualization of any DNA target: Proven “double-Z” probe design and signal amplification system of RNAscope, enabling rapid and flexible probe development
    • ​Unparalleled performance in detecting small genomic regions/single gene locus ​- Amplify target-specific signals but not background noise from non-specific hybridization
    • Easy Interpretation and high throughput data analysis - Permanent chromogenic signal and superior morphological context
    Dates and Registration:
    April
    27
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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