• Jul
    19
    Get More Data from Every Tissue Sample; Increasing Efficiency of Your RNA-ISH Studies
    Hailing Zong, Ph.D., Scientist, Advanced Cell Diagnostics
    Victoria Duckworth, Product Manager, PerkinElmer
    Get More Data from Every Tissue Sample; Increasing Efficiency of Your RNA-ISH Studies
    Hailing Zong, Ph.D.
    Scientist, Advanced Cell Diagnostics
    Victoria Duckworth
    Product Manager, PerkinElmer
    Outline:

    In this webinar you will learn how to achieve single-molecule detection of several RNA targets simultaneously and perform co-localization studies of any expressed gene set in nearly any tissue type, while enabling additional capabilities including dual ISH-IHC for RNA and protein co-expression. We will discuss combining the sensitivity of Advanced Cell Diagnostic’s RNAscope Multiplex Fluorescent assays detected with PerkinElmer’s Opal technology and the multispectral imaging capability of PerkinElmer’s Vectra and Mantra Multispectral Quantitative Pathology Imaging instruments.

    Dates and Registration:
    July
    19
    9 am PST
    Register Now
  • Jul
    25
    Single-Molecule RNA in situ Hybridization Assays for The Detection Of mRNA, ncRNA, And Exon Junctions In FFPE Tissues
    Courtney Anderson, Ph.D., Applications Leader, Senior Scientist, Advanced Cell Disagnostics
    Single-Molecule RNA in situ Hybridization Assays for The Detection Of mRNA, ncRNA, And Exon Junctions In FFPE Tissues
    Courtney Anderson, Ph.D.
    Applications Leader, Senior Scientist, Advanced Cell Disagnostics
    Outline:

    RNA is a key biomarker of the dynamic gene expression changes occurring in cells and tissues. Compared to techniques that analyze bulk tissue, identifying RNA expression at the single cell level within the morphological context provides valuable spatial information on gene expression. In this webinar we will present the advanced single-cell, single-molecule RNA in situ hybridization (ISH) technology RNAscope. We will discuss the technology, how to perform the assay, how to analyze and quantify the results, and provide multiple applications of the technology in both academic and clinical research settings.

    Dates and Registration:
    July
    25
    1:00 pm PST
    Register Now
  • Jul
    31
    RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS
    Sneha Palliyil, PhD., Technical Support Scientist
    RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS
    Sneha Palliyil, PhD.
    Technical Support Scientist
    Outline:

    In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.
    Please bring your RNAscope® technology questions to the webinar and our team will answer them live by chat.

    Who should attend:

    This webinar will benefit both new and experienced RNAscope® technology users.

    Learning Objectives:
    • How RNAscope® and BaseScope™ assays work
    • What you need to get started
    • Tips and tricks for successful sample preparation
    • Examples of staining results & research application specific data
    • FAQs & Troubleshooting tips
    Dates and Registration:
    July
    31
    Register Now
  • Aug
    02
    Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
    Omer Bayraktar, Ph.D., Wellcome Sanger Institute
    Xiao-Jun Ma, Ph.D., Chief Scientific Officer, Advanced Cell Diagnostics
    Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
    Omer Bayraktar, Ph.D.
    Wellcome Sanger Institute
    Xiao-Jun Ma, Ph.D.
    Chief Scientific Officer, Advanced Cell Diagnostics
    Outline:

    Sequencing of RNA (RNASeq) can provide a trove of useful information for quantification and characterization of the transcriptome. During bulk sequencing, however, spatial and cellular heterogeneity information is lost. Enter single-cell–level analysis, which can provide unprecedented detail on cellular diversity in development, health, and disease. This emerging area of spatial transcriptomics aims to map a single-cell–level molecular atlas of human tissues in situ. However, mapping entire tissue areas at single-cell quantitative resolution presents significant challenges for imaging and data analysis. In this webinar, efforts to develop large-scale in situ transcriptomics will be described, using the mouse brain as a model system for mapping the molecular diversity of neuronal and glial cell types. The experimental workflow includes automated tissue processing, imaging and analysis for large-scale multiplex mapping, and quantification of gene expression across brain regions in situ at single-cell resolution. The technology that makes this high-resolution mapping possible will be described and compared with other current methods.

    Learning Objectives:

    During the webinar, the speakers will:

    • Describe the power and usefulness of in situ transcriptomics
    • Discuss the application of this technology to large-scale tissue mapping in the mouse brain
    • Explain how in situ transcriptomics (using RNAScope technology) can complement RNASeq and single-cell RNASeq
    • Answer audience questions during the live broadcast!

    To learn more about products or technologies related to this webinar, go to http://rna.acdbio.com/transcriptomics.

    This webinar will last for approximately 60 minutes.

    Dates and Registration:
    August
    02
    9 am PST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

Presentations Download File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

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