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  • Jan
    28
    2021 Jan 28: ACD PAS Webinar: PAS Webinar: RNAscope ISH services for Gene Therapy Biodistribution
    Christopher Bunker, Ph.D., MBA, Senior Director, Business Development, Advanced Cell Diagnostics
    2021 Jan 28: ACD PAS Webinar: PAS Webinar: RNAscope ISH services for Gene Therapy Biodistribution
    Christopher Bunker, Ph.D., MBA
    Senior Director, Business Development, Advanced Cell Diagnostics
    Outline:

    Visual detection and quantification of AAV and LV vector biodistribution and transgene expression in preclinical animal models with RNAscope™ and BaseScope™ in situ hybridization.

    RNAscope in situ hybridization (ISH) has been described by its users as a means of “morphology-based AAV biodistribution analysis.” RNAscope in situ assays of AAV DNA and transgene mRNA combine the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology. By combined RNAscope probes to AAV vector and transgene mRNA, vector copy number and expression can be visually assessed and quantified simultaneously in each cell of an intact fixed tissue. With the addition of cell marker probes in multiplex fluorescent RNAscope assays, AAV vector and transcript levels can be measured in any target cell type.
    BaseScope ISH is another powerful tool facilitating gene therapy development.  Specific measurement of therapeutic human transgene expression in animal models is often impossible by IHC because of the high level of homology between transgene and animal ortholog transcripts.  BaseScope enables specific detection of even single nucleotide differences and can easily differentiate AAV human transgene from non-human primate and other animal ortholog sequences.
    These assays are enabling more informative and efficient preclinical gene therapy development and contributing critical data in IND-enabling studies

    Learning Objectives:
    Key features of RNAscope gene therapy assays:
    Simultaneous AAV vector biodistribution and transgene mRNA expression analysis
    Quantifiable, cell-specific measurement of AAV and transgene in multiplexed assays with cell markers
    Rapid, visual germline transmission safety assessment in AAV assays on intact gonadal tissues
    Dates and Registration:
    January
    28
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Feb
    02
    2021 Feb 2 - Knowledge Resource Series - COVID-19 Applications
    Jyoti Phatak-Sheldon, M.S., Associate Scientist - Applications, Advanced Cell Diagnostics
    2021 Feb 2 - Knowledge Resource Series - COVID-19 Applications
    Jyoti Phatak-Sheldon, M.S.
    Associate Scientist - Applications, Advanced Cell Diagnostics
    Outline:

    Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) technology is a powerful tool to establish the etiology and pathogenesis of viral disease. Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity, and speed. Beyond this, RNAscope ISH technology uniquely offers a molecular detection at a single cell resolution coupled with morphological context to enable visualization of the virus in different infected tissues and cell types. In this webinar, we will demonstrate how RNAscope technology can be used to detect viral infection and pathogenesis for research and enhance our understanding of globally important SARS-CoV2 virus.

    Learning Objectives:
    • RNAscope Technology Overview
    • Literature review showing how COVID-19 Detection and Probes deliver the results researchers seek
    • Learn about the various COVID-19 Offerings (Bio-Techne) to further support your research findings in applications including viral detection, acquired immunity, vaccine efficacy and many more. 
    Dates and Registration:
    February
    02
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Feb
    03
    2021 Feb 3 - ACD | miRNAscope Webinar
    Helly Pimentel, Product Manager, Advanced Cell Diagnostics
    Miguel Mata, Ph.D., Scientist, Advanced Cell Diagnostics
    2021 Feb 3 - ACD | miRNAscope Webinar
    Helly Pimentel
    Product Manager, Advanced Cell Diagnostics
    Miguel Mata, Ph.D.
    Scientist, Advanced Cell Diagnostics
    Outline:

    Get a deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the robust RNA in situ hybridization assay that enables the detection of ASOs, miRNAs, siRNAs, and other smaller RNAs (16-50nt) with spatial and morphological context at single cell resolution.

    Learning Objectives:
    • Introduction to the miRNAscope technology and workflow 
    • Key applications that necessitate the use of the miRNAscope Assay 
    • Key benefits of the miRNAscope Assay for your research 
    Dates and Registration:
    February
    03
    10am PST
    1pm EST
    Register Now
  • Feb
    04
    2021 Feb 4 - ACD | Rutgers Cancer Institute of New Jersey
    David Remedios, Sales Development Representative, Advanced Cell Diagnostics
    Emily Martersteck, M.S.,, Field Application Scientist (FAS), Advanced Cell Diagnostics
    Josh Vieth, Ph.D., Managing Director, Immune Monitoring & Advanced Genomics, Rutgers Cancer Institute of New Jersey
    2021 Feb 4 - ACD | Rutgers Cancer Institute of New Jersey
    David Remedios
    Sales Development Representative, Advanced Cell Diagnostics
    Emily Martersteck, M.S.,
    Field Application Scientist (FAS), Advanced Cell Diagnostics
    Josh Vieth, Ph.D.
    Managing Director, Immune Monitoring & Advanced Genomics, Rutgers Cancer Institute of New Jersey
    Outline:

    Using RNAscope ISH to detect cancer and immune markers in the tumor microenvironment at single-cell resolution

    The study of the tumor microenvironment (TME) expands the understanding of cancer initiation, progression, and metastases. The RNAscope assay is an ideal solution to analyze specific cell types and states infiltrated in the TME within the morphological context. 

    With RNAscope you can identify and characterize immune cell types, cytokines, chemokines, transcription factors, checkpoint markers, and more, all at the single cell level.

    Dates and Registration:
    February
    04
    10am PST
    1pm EST
    Register Now
  • Feb
    10
    2021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease Applications
    Anushka Dikshit, Ph.D., Applications Scientist, Genomics, Advanced Cell Diagnostics
    2021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease Applications
    Anushka Dikshit, Ph.D.
    Applications Scientist, Genomics, Advanced Cell Diagnostics
    Outline:

    The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot visualizing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples.

    The RNAscope™ technology provides pivotal single-cell information that is necessary to study novel cell populations in vital organs such as the liver and kidneys. Studying gene expression with spatial resolution can provide functional information for cellular sub-populations in turn improving our understanding of various disease pathologies.

    Learning Objectives:
    • Understand complex spatial relationship among different cell types within the liver and kidneys.
    • Identify and characterize novel cell types .
    • Study expression of target genes implicated is liver and kidney diseases.
    Dates and Registration:
    February
    10
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Feb
    11
    2021 Feb 11 - ACD PAS RNAscope ISH Services for Biomarker Assay Development
    Elizabeth Kim, Research Associate, Pharma Assay Services, Advanced Cell Diagnostics
    Christian Reyes, Team Lead, Pharma Assay Services, Advanced Cell Diagnostics
    2021 Feb 11 - ACD PAS RNAscope ISH Services for Biomarker Assay Development
    Elizabeth Kim
    Research Associate, Pharma Assay Services, Advanced Cell Diagnostics
    Christian Reyes
    Team Lead, Pharma Assay Services, Advanced Cell Diagnostics
    Outline:

    This webinar will provide an overview of how Bio-Techne’s Assay Services’ Team expertise in RNAscope technology is being utilized by Biopharma for the development of biomarkers and companion diagnostic assays to support their therapeutic programs. Advanced Cell Diagnostics’ RNAscopeTM in situ hybridization (ISH) platforms are widely utilized for high resolution tissue-based and cell-specific analysis of targets and biomarkers. The RNAscope ISH platform is clinically validated and enables quantitative expression analysis that has repeatedly been proven to better stratify treatment-responsive and non-responsive patients in therapeutic clinical trials compared to traditional methods such as IHC and qPCR. RNAscope is often more sensitive than IHC and provides cell-specific biomarker data in complex tissue microenvironments that qPCR cannot.

    Learning Objectives:
    • Biomarker validation to accelerate translation of assays into clinical development in a GCLP setting in a matter of 4-6 weeks*
    • Assay feasibility and development - sensitivity, specificity, and precision testing (repeatability & reproducibility)
    • Development of pathologist scoring and HALOTM image analysis algorithms for biomarker assessment.
    • Established protocols for clinical assay transfer to CLIA-certified labs for prospective use.
    • RNAscope multiplexing and validated protocols for co-detection of biomarkers and immune cell markers
    • Quantifiable, cell-specific, single-molecule detection of RNA biomarkers in tumor biopsies
    • Superior performance against traditional methods, e.g., IHC, qPCR
    • Application in small molecule, biologic, and cell therapy biomarkers
    Dates and Registration:
    February
    11
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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