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  • Jun
    22
    2021 June 22 - Resolving Brain Tissues with Spatial Transcriptomics: A Virtual Roundtable Discussion
    Omer Bayraktar, PhD, Group Lead, Sanger Wellcome
    Gregory Carter, PhD, Associate Professor, The Bernard and Lusia Milch Endowed Chair, The Jackson Laboratory
    Wei-Ting Chen, PhD, Staff Scientist, KU Leuven
    Kristen Maynard, PhD, Research Scientist, Lieber Institute for Brain Development
    2021 June 22 - Resolving Brain Tissues with Spatial Transcriptomics: A Virtual Roundtable Discussion
    Omer Bayraktar, PhD
    Group Lead, Sanger Wellcome
    Gregory Carter, PhD
    Associate Professor, The Bernard and Lusia Milch Endowed Chair, The Jackson Laboratory
    Wei-Ting Chen, PhD
    Staff Scientist, KU Leuven
    Kristen Maynard, PhD
    Research Scientist, Lieber Institute for Brain Development
    Outline:

    New technologies are providing spatial context for cells and their marker genes at ever-higher scale, sensitivity, and resolution. In the brain, where cellular organization is of utmost importance, these methods are enabling researchers to ask new kinds of questions and tackle the toughest problems in biology. From creating high-level atlases to examining the role of genes in cellular processes related to diseases such as Alzheimer’s, spatial transcriptomics is ushering in a new wave of approaches in neuroscience research.  

    Join us as we hear from four of the leading experts on the use of spatial transcriptomics methods in the brain. Our panelists will discuss their own work, the methods they have used, and the new possibilities available to researchers in their fields. At the end, we’ll have a live question and answer session featuring submissions from our audience.  

    Dates and Registration:
    June
    22
    7am PST
    10am EST
    4pm CEST
    Register Now
  • Jun
    28
    2021 June 28 - A new era of RNA detection: Exploring new technologies and prospects
    Boye Schnack Nielsen, Ph.D.,, Manager of Molecular Histology Bioneer, Hørsholm, Denmark.
    2021 June 28 - A new era of RNA detection: Exploring new technologies and prospects
    Boye Schnack Nielsen, Ph.D.,
    Manager of Molecular Histology Bioneer, Hørsholm, Denmark.
    Outline:

    In addition to commonly studied messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), multiple types of RNAs have become evident over recent decades, including microRNA (miRNA), long non-protein coding RNA (lncRNA) and circular RNA (circRNA). However, the functions of these RNA types within our cells are not yet fully understood.



    With the development of new in situ detection technologies, RNA in situ hybridization has now been enrolled in diagnostics applications, enabling the visualization of RNA localization in tissues and cells to better address their roles in the tissue microenvironment and in molecular pathways.



    Join this webinar, with Dr. Boye Schnack Nielsen, Manager of Molecular Histology at Bioneer, to learn more about:

    Learning Objectives:
    • Our understanding of different RNA types
    • RNA in situ hybridization
    • Combined RNA and protein staining
    Dates and Registration:
    June
    28
    8am PST
    11am EST
    5pm CEST
    Register Now
  • Jun
    29
    2021 June 29 - ACD Support Webinar - Probe Design Overview & Applications
    Betty Booker, Ph.D.,, Senior Scientist, Research & Development, Advanced Cell Diagnostics, a Bio-Techne brand
    2021 June 29 - ACD Support Webinar - Probe Design Overview & Applications
    Betty Booker, Ph.D.,
    Senior Scientist, Research & Development, Advanced Cell Diagnostics, a Bio-Techne brand
    Outline:

    Overview of the RNAscope/BaseScope probe design strategies to achieve highly specific and sensitive detection of a variety of RNA targets in situ, including but not limited to RNA, mRNA, pre-RNA, TCR/BCR, Knockout validation and splice variant.

     
    Learning Objectives:
    • Introduction to the RNAscope/BaseScope/miRNAscope/DNAscope design workflow. 
    • Featured applications of RNAscope/BaseScope/miRNAscope/DNAscope probes including detection of RNA, mRNA, pre-RNA, mature microRNA, ASO, siRNA, TCR/BCR, Knockout validation, splice variants, DNA copy number and structural variations. 
    • Frequently asked questions - Applications (FAQs).
    Dates and Registration:
    June
    29
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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