• Apr
    04
    Introduction to the RNAscope® Probes: Identifying the right probe for your research
    Morgane Rouault, PhD, Application and Support Scientist, Europe, Advanced Cell Diagnostics, Inc.
    Introduction to the RNAscope® Probes: Identifying the right probe for your research
    Morgane Rouault, PhD
    Application and Support Scientist, Europe, Advanced Cell Diagnostics, Inc.
    Outline:

    RNAscope® assay is an innovative and proprietary RNA in situ hybridization (ISH) assay based on ACD's patented technology with signal amplification and simultaneous background noise suppression. RNAscope® assay delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. 



    During this webinar we will present how to identify the right probe for your target from catalog probes, discuss our probe design technology and nomenclature. In addition we will also learn from our Design Scientist about the target information required for designing a custom probe suitable for your research needs. It is intended to be an introduction and does not require previous training.

    Learning Objectives:

    This webinar will include the following topics: 

    • Probe Design Technology Basics
    • RNAscope Probe Nomenclature
    • Navigating the website for obtaining the right probe information
    • Examples of Custom Probe Design for scientific applications eg KO mice, Xenograft
    • Review the information needed in the Custom Probe Design Form
    Dates and Registration:
    April
    04
    4 PM CET
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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