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  • Mar
    10
    2021 Mar 10 - Webinar: Spatial Expression Analysis Elucidates Mechanisms of circRNAs in Cancer
    Lasse Sommer Kristensen, PhD, Associate Professor, Department of Biomedicin, Aarhus University,
    Anushka Dikshit, Ph.D., Applications Scientist, Advanced Cell Diagnostics
    2021 Mar 10 - Webinar: Spatial Expression Analysis Elucidates Mechanisms of circRNAs in Cancer
    Lasse Sommer Kristensen, PhD
    Associate Professor, Department of Biomedicin, Aarhus University,
    Anushka Dikshit, Ph.D.
    Applications Scientist, Advanced Cell Diagnostics
    Outline:
    Circular RNAs (circRNAs) have recently gained substantial attention in the cancer research field where most, including the putative oncogene ciRS-7 (CDR1as), have been proposed to function as competitive endogenous RNAs (ceRNAs) by “sponging” specific microRNAs.


    In this webinar, Lasse Sommer Kristensen of Aarhus University will describe his team’s work in studying the role of circRNAs in cancer. His team recently reported the first spatially resolved cellular expression patterns of ciRS-7 in colon cancer. Surprisingly, they found that ciRS-7 is completely absent in the cancer cells, but highly expressed in stromal cells within the tumor microenvironment.


    In addition, they proposed a model to explain correlations between circRNA and mRNA expression, which are commonly interpreted as evidence of a ceRNA function, based on different cancer-to-stromal cell ratios among the studied tumor specimens.


    Together, these results have wide implications for future circRNA studies and highlight the importance of spatially resolving expression patterns of circRNAs proposed to function as ceRNAs.
    Learning Objectives:
    Key points to be discussed in this webinar:
     
    • The circular RNA named ciRS-7 is overexpressed in human cancers; however, in many types of cancer it is not expressed in the cancer cells, but abundant in stromal cells within the tumors
    • Cancer-to-stromal cell ratios contribute to correlations between circRNAs and microRNA target genes
    • Analyses of spatial expression patterns are key to understanding molecular mechanisms of circRNAs in cancer
    Dates and Registration:
    March
    10
    8am PST
    11am EST
    5pm CEST
    Register Now
  • Mar
    17
    2021 Mar 17 - Oncofetal ecosystem: space, time and memory
    Ankur Sharma, Ph.D.,, Laboratory Head at Harry Perkins Institute of Medical Research and women’s cancer senior fellow/Senior lecturer at Curtin Health Innovation Research Institute (CHIRI), Curtin University, Perth, Australia.
    Anushka Dikshit, Ph.D., Applications Scientist, Advanced Cell Diagnostics
    2021 Mar 17 - Oncofetal ecosystem: space, time and memory
    Ankur Sharma, Ph.D.,
    Laboratory Head at Harry Perkins Institute of Medical Research and women’s cancer senior fellow/Senior lecturer at Curtin Health Innovation Research Institute (CHIRI), Curtin University, Perth, Australia.
    Anushka Dikshit, Ph.D.
    Applications Scientist, Advanced Cell Diagnostics
    Outline:

    This webcast will describe employing single cell genomics and spatial transcriptomics to discover a previously unexplored oncofetal reprogramming of the tumor ecosystem.

    Analysis of ~212,000 cells representing human fetal, hepatocellular carcinoma (HCC), and mouse liver revealed remarkable fetal-like reprogramming of the tumor microenvironment. Specifically, the HCC ecosystem displayed features reminiscent of fetal development, including re-emergence of fetal-associated endothelial cells and fetal-like tumor-associated macrophages.

    The speaker will describe a shared immunosuppressive oncofetal ecosystem in fetal liver and HCC and its potential implications for targeting therapeutic interventions in HCC and identifying similar paradigms in other cancers and diseases.

    Learning Objectives:
    • Identification of fetal-associated endothelial cells and macrophages in HCC
    • Shared oncofetal ecosystem between human fetal liver and HCC
    • The phenomenon of oncofetal reprogramming in the tumor ecosystem
    Dates and Registration:
    March
    17
    10am SGT
    7pm PST
    10pm EST
    3am CEST
    Register Now
  • Mar
    18
    2021 Mar 18 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression Analysis
    Mansi Garg, M.S., Sr. Manager, Project Operations Advanced Cell Diagnostics, a Bio-Techne brand
    Ruby Hsu, Ph.D.,, Director, Business Development Advanced Cell Diagnostics, a Bio-Techne brand
    2021 Mar 18 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression Analysis
    Mansi Garg, M.S.
    Sr. Manager, Project Operations Advanced Cell Diagnostics, a Bio-Techne brand
    Ruby Hsu, Ph.D.,
    Director, Business Development Advanced Cell Diagnostics, a Bio-Techne brand
    Outline:
    This webinar will introduce our RNAscope in situ hybridization (ISH) services, as well as our quantitative image analysis capabilities and quality-controlled tissue bank for target validation.


    Bio-Techne’s Professional Assay Services (PAS) is the only CRO specialized in RNAscope, BaseScope™ and miRNAscope™ ISH services. We provide tissue-based and cell specific analysis of targets and biomarkers in as little as 4 weeks.



    Please join us to hear how our services are advancing the research and drug development at hundreds of academic and clinical institutions worldwide. Hear examples of studies in immuno-oncology, cell and gene therapy, neuroscience, single-cell RNAseq validation, biomarker validation, and more.
    Learning Objectives:
    • Visualize the cellular heterogeneity of tissues and solid tumors with the multiplexing and spatial capabilities of the RNAscope Multiplex Fluorescent assay
    • Specific and sensitive biomarker validation to accelerate translation of assays into the clinic
    • Morphology-based biodistribution analysis of AAV vector and transgene expression
    • CAR-T and TCR-T cell tumor infiltration and activity assays
    • Detection of splice variants, point mutations and short target sequences with BaseScope ISH assays
    • Co-stain with cell-type markers using our ISH/IHC Co-Detection workflow
    • PAS’s quality-controlled tissue bank including solid tumors, diseased and normal human tissues, and TMAs
    • RNAscope image analysis offerings for quantitative approaches to scoring
    Dates and Registration:
    March
    18
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Mar
    19
    2021 Mar 19 - Webinar: Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples
    Shyam Solanki, Ph.D., Postdoctoral Research Associate, Dept. of Crop and Soil Sciences Washington State University
    Jyoti Phatak-Sheldon, MS, Associate Scientist, Advanced Cell Diagnostics
    2021 Mar 19 - Webinar: Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples
    Shyam Solanki, Ph.D.
    Postdoctoral Research Associate, Dept. of Crop and Soil Sciences Washington State University
    Jyoti Phatak-Sheldon, MS
    Associate Scientist, Advanced Cell Diagnostics
    Outline:
    Join us as we discuss how this modified protocol on the plant leaf tissues can be adapted easily on other plant species and provides a powerful tool of RNAscope for RNA visualization.



    RNAscope multiplex fluorescent RNA-ISH detection is a powerful and sensitive tool for spatio-temporal RNA species detection in tissues. However, due to plant tissues' morphological and structural characteristics, its implication was not reported until Solanki et al., 2020 (https://doi.org/10.1186/s13007-020-00614-4) presented a modified protocol of tissue preparation and probe hybridization. This modified protocol on the barley leaf tissues can be adapted easily on other plant species and provides a powerful tool for plant researchers and cell biologists.
     
    In this webinar, we will discuss a practical approach of carrying out RNAscope assay using a parallel comparison of plant and animal samples to avoid common pitfalls and increase the chances of a successful experiment.
    Learning Objectives:
    • Tissue preparation
    • Tissue pretreatments
    • RNAscope V2 assay
    • Sample visualization and image analysis
    Dates and Registration:
    March
    19
    8am PST
    11am EST
    5pm CEST
    Register Now
  • Mar
    30
    2021 Mar 30 - ACD Support Webinar: Visualization and Analysis of RNAscope™ Results using QuPath
    Adeola Adeyemo, Ph.D., Technical Support Scientist, Advanced Cell Diagnostics
    Ching-Wei Chang, Ph.D., Senior Image Analysis Scientist Advanced Cell Diagnostics, a Bio-Techne brand
    2021 Mar 30 - ACD Support Webinar: Visualization and Analysis of RNAscope™ Results using QuPath
    Adeola Adeyemo, Ph.D.
    Technical Support Scientist, Advanced Cell Diagnostics
    Ching-Wei Chang, Ph.D.
    Senior Image Analysis Scientist Advanced Cell Diagnostics, a Bio-Techne brand
    Outline:
    The RNAscope in situ hybridization (ISH) technology is a powerful method for detection of gene expression within the spatial and morphological tissue context at the single molecule level.


    This webinar will focus on how to analyze RNAscope, BaseScope™, and miRNAscope™ data quantitatively using QuPath open-source software.

    A QuPath Demo will guide users through data analysis of both chromogenic and fluorescent images and in selecting the best method for quantifying their images.


    Topics covered during this webinar include nuclear and cellular segmentation, color deconvolution, probe dot detection and quantification, classification of cells, visualization of results and scripting of the workflow for batch analysis.
    Learning Objectives:
    • Overview of RNAscope technology and general imaging guidelines
    • Introduction of QuPath for RNAscope ISH image analysis
    • Workflow for quantitation of both chromogenic and fluorescent staining
    • Examples of RNAscope, BaseScope, and miRNAscope QuPath analysis
    Dates and Registration:
    March
    30
    10am PST
    1pm EST
    7pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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