- Jan262021 Jan 26: ACD Support Webinar - Overview of the RNAscope Technology Based on Key Applications2021 Jan 26: ACD Support Webinar - Overview of the RNAscope Technology Based on Key ApplicationsAdeola Adeyemo, Ph.D.Technical Support Scientist, Advanced Cell DiagnosticsOutline:
The RNAscope in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary probe design, in combination with the advanced signal amplification enables highly specific and sensitive detection of target RNA with each dot representing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples. In this webinar, we will review various ACD product lines and discuss the utilization of each product line for specific applications.
Learning Objectives:- Introduction to the various product lines
- Key applications that necessitate the use of the RNAscope, BaseScope™ and miRNAscope assays
Dates and Registration: - Jan282021 Jan 28: ACD PAS Webinar: PAS Webinar: RNAscope ISH services for Gene Therapy Biodistribution2021 Jan 28: ACD PAS Webinar: PAS Webinar: RNAscope ISH services for Gene Therapy BiodistributionChristopher Bunker, Ph.D., MBASenior Director, Business Development, Advanced Cell DiagnosticsOutline:
Visual detection and quantification of AAV and LV vector biodistribution and transgene expression in preclinical animal models with RNAscope™ and BaseScope™ in situ hybridization.
RNAscope in situ hybridization (ISH) has been described by its users as a means of “morphology-based AAV biodistribution analysis.” RNAscope in situ assays of AAV DNA and transgene mRNA combine the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology. By combined RNAscope probes to AAV vector and transgene mRNA, vector copy number and expression can be visually assessed and quantified simultaneously in each cell of an intact fixed tissue. With the addition of cell marker probes in multiplex fluorescent RNAscope assays, AAV vector and transcript levels can be measured in any target cell type.
BaseScope ISH is another powerful tool facilitating gene therapy development. Specific measurement of therapeutic human transgene expression in animal models is often impossible by IHC because of the high level of homology between transgene and animal ortholog transcripts. BaseScope enables specific detection of even single nucleotide differences and can easily differentiate AAV human transgene from non-human primate and other animal ortholog sequences.
These assays are enabling more informative and efficient preclinical gene therapy development and contributing critical data in IND-enabling studiesLearning Objectives:Key features of RNAscope gene therapy assays:• Simultaneous AAV vector biodistribution and transgene mRNA expression analysis• Quantifiable, cell-specific measurement of AAV and transgene in multiplexed assays with cell markers• Rapid, visual germline transmission safety assessment in AAV assays on intact gonadal tissuesDates and Registration: - Feb022021 Feb 2 - Knowledge Resource Series - COVID-19 Applications2021 Feb 2 - Knowledge Resource Series - COVID-19 ApplicationsJyoti Phatak-Sheldon, M.S.Associate Scientist - Applications, Advanced Cell DiagnosticsOutline:
Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) technology is a powerful tool to establish the etiology and pathogenesis of viral disease. Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity, and speed. Beyond this, RNAscope ISH technology uniquely offers a molecular detection at a single cell resolution coupled with morphological context to enable visualization of the virus in different infected tissues and cell types. In this webinar, we will demonstrate how RNAscope technology can be used to detect viral infection and pathogenesis for research and enhance our understanding of globally important SARS-CoV2 virus.
Learning Objectives:- RNAscope Technology Overview
- Literature review showing how COVID-19 Detection and Probes deliver the results researchers seek
- Learn about the various COVID-19 Offerings (Bio-Techne) to further support your research findings in applications including viral detection, acquired immunity, vaccine efficacy and many more.
Dates and Registration: - Feb032021 Feb 3 - ACD | miRNAscope Webinar2021 Feb 3 - ACD | miRNAscope WebinarHelly PimentelProduct Manager, Advanced Cell DiagnosticsMiguel Mata, Ph.D.Scientist, Advanced Cell DiagnosticsOutline:
Get a deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the robust RNA in situ hybridization assay that enables the detection of ASOs, miRNAs, siRNAs, and other smaller RNAs (16-50nt) with spatial and morphological context at single cell resolution.
Learning Objectives:- Introduction to the miRNAscope technology and workflow
- Key applications that necessitate the use of the miRNAscope Assay
- Key benefits of the miRNAscope Assay for your research
Dates and Registration: - Feb042021 Feb 4 - ACD | Rutgers Cancer Institute of New Jersey2021 Feb 4 - ACD | Rutgers Cancer Institute of New JerseyDavid RemediosSales Development Representative, Advanced Cell DiagnosticsEmily Martersteck, M.S.,Field Application Scientist (FAS), Advanced Cell DiagnosticsJosh Vieth, Ph.D.Managing Director, Immune Monitoring & Advanced Genomics, Rutgers Cancer Institute of New JerseyOutline:
Using RNAscope ISH to detect cancer and immune markers in the tumor microenvironment at single-cell resolution
The study of the tumor microenvironment (TME) expands the understanding of cancer initiation, progression, and metastases. The RNAscope assay is an ideal solution to analyze specific cell types and states infiltrated in the TME within the morphological context.
With RNAscope you can identify and characterize immune cell types, cytokines, chemokines, transcription factors, checkpoint markers, and more, all at the single cell level.
Dates and Registration: - Feb102021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease Applications2021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease ApplicationsAnushka Dikshit, Ph.D.Applications Scientist, Genomics, Advanced Cell DiagnosticsOutline:
The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot visualizing a single RNA transcript. This robust signal-to-noise technology allows for the detection of gene transcripts at the single molecule level with single-cell resolution and can further expand our understanding of gene expression in cell lines and tissues samples.
The RNAscope™ technology provides pivotal single-cell information that is necessary to study novel cell populations in vital organs such as the liver and kidneys. Studying gene expression with spatial resolution can provide functional information for cellular sub-populations in turn improving our understanding of various disease pathologies.
Learning Objectives:- Understand complex spatial relationship among different cell types within the liver and kidneys.
- Identify and characterize novel cell types .
- Study expression of target genes implicated is liver and kidney diseases.
Dates and Registration: - Feb112021 Feb 11 - ACD PAS RNAscope ISH Services for Biomarker Assay Development2021 Feb 11 - ACD PAS RNAscope ISH Services for Biomarker Assay DevelopmentElizabeth KimResearch Associate, Pharma Assay Services, Advanced Cell DiagnosticsChristian ReyesTeam Lead, Pharma Assay Services, Advanced Cell DiagnosticsOutline:
This webinar will provide an overview of how Bio-Techne’s Assay Services’ Team expertise in RNAscope technology is being utilized by Biopharma for the development of biomarkers and companion diagnostic assays to support their therapeutic programs. Advanced Cell Diagnostics’ RNAscopeTM in situ hybridization (ISH) platforms are widely utilized for high resolution tissue-based and cell-specific analysis of targets and biomarkers. The RNAscope ISH platform is clinically validated and enables quantitative expression analysis that has repeatedly been proven to better stratify treatment-responsive and non-responsive patients in therapeutic clinical trials compared to traditional methods such as IHC and qPCR. RNAscope is often more sensitive than IHC and provides cell-specific biomarker data in complex tissue microenvironments that qPCR cannot.
Learning Objectives:- Biomarker validation to accelerate translation of assays into clinical development in a GCLP setting in a matter of 4-6 weeks*
- Assay feasibility and development - sensitivity, specificity, and precision testing (repeatability & reproducibility)
- Development of pathologist scoring and HALOTM image analysis algorithms for biomarker assessment.
- Established protocols for clinical assay transfer to CLIA-certified labs for prospective use.
- RNAscope multiplexing and validated protocols for co-detection of biomarkers and immune cell markers
- Quantifiable, cell-specific, single-molecule detection of RNA biomarkers in tumor biopsies
- Superior performance against traditional methods, e.g., IHC, qPCR
- Application in small molecule, biologic, and cell therapy biomarkers
Dates and Registration:
You can watch a video or download presentation of the past webinars.
- 2021 Jan 20 Knowledge Resource Webinar Series - Neurodegenerative Disease Applications
- 2021 Jan 14 Knowledge Resource Webinar Series - Oncology Applications
- 2020 Dec 17: ACD Support Webinar - Best of 2020 Applications
- 2020 Dec 15: PAS Webinar: Use of RNAscope ISH services for T-Cell Therapy Detection & Quantification
- 2020 Dec 2 Knowledge Resource Webinar Series - Applications Using BaseScope
- 2020 Dec 1: ACD Support Webinar - Tips and Tricks for Getting Started with miRNAscope™ Assay
- 2020 Nov 17 - ACD Webinar: Now Available to Academic Labs - RNAscope ISH Services for Spatial Mapping of Gene Expression
- 2020 Nov 12 - ACD Neuro Summit
- 2020 Nov 2 - Knowledge Resource Series - Single-Cell Applications
- 2020 Nov 17 - Spatial Expression Analyses Elucidates Mechanisms of circRNAs in Cancer
- 2020 Nov 20 - Companion Diagnostic and Biomarker Assay Development for Interrogation of Liquid and Solid Tumor Biopsies – From RNAscope to Exosomes
- 2020 Oct 27 - Visualize your RNA and RNA-Protein Co-Detection using RNAscope™ Multiplex Fluorescent V2 Assay
- 2020 Oct 26 - Knowledge Resource Series - Inflammation Applications
- 2020 Oct 23 - Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples
- 2020 Oct 19 - Knowledge Resource Series - Pain Medicine
- 2020 Oct 6 - Longitudinal Immune Profiling Reveals Key Immune Signatures Associated with COVID-19
- 2020 Sept 29: Introducing ACD's New RNA-Protein Co-Detection Assays
- 2020 Sept 22 - Knowledge Resource Series - Stem Cell Applications Webinar
- 2020 Sept 21 - Resolving the fibrotic niche – a single-cell RNA sequencing approach
- 2020 Sept 4 Incorporation and Automation of RNAscope Technology into your COVID-19 Viral Pathogenesis Research
- 2020 Sept 17 Webinar - Understanding Pain Mechanisms: RNA Expression Analysis of Peripheral Nervous System Tissue
- 2020 Sept 9 Webinar - Spatial Mapping and in situ Confirmation of Gene Signatures using RNAscope HiPlex Technology
- 2020 Aug 31 - Knowledge Resource Series - Spatial Genomics Applications Webinar
- 2020 Sept 15 ACD Sales Webinar - Accelerate target validation using the RNAscope ISH automated assay platform
- 2020 Sept 10 - Clinical Assay Development using ACD’s in situ Hybridization Assay Services
- 2020 Sept 2 - Spatial Profiling to Assess a Novel Gene Therapy in a Mouse Model of a Rare Genetic Disorder
- 2020 August 24 - Knowledge Resource Series - Diagnostic Applications Webinar
- 2020 Aug 6 - Spatial Mapping of Gene Expression in Tumors Using RNAscope Technology
- 2020 Aug 25 - RNAscope® Automated Assays Solutions: Leica BOND RX System and Roche Discovery Ultra.
- Characterizing Complex Tissues with Spatial Expression Analysis Using the RNAscope Technology
- 2020 Aug 12 - Solving Cell and Gene Therapy Hurdles with Innovative Tools
- 2020 July 28 - miRNAscope Launch Webinar: Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
- 2020 Aug 18 - Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
- 2020 July 6 - Knowledge Resource Series #4 COVID & Infectious Disease Webinar
- 2020 July 14 - Viral Detection by ACD’s in situ Hybridization Assay Services
- 2020 June 23 - Knowledge Resource Series #3 Biomarker Development Webinar
- Spatial mapping and in situ confirmation of gene signatures using RNAscope HiPlex Technology
- 2020 June 24: Understanding pain mechanisms: RNA expression analysis of peripheral nervous system tissue
- 2020 Jun 16 - Knowledge Resource Series#2 Gene Therapy Webinar
- 2020 Jun 19 - Probes: An Overview of Probe Design Technology, Application and New Probe Requests
- 2020 Jun 9 - Knowledge Resource Series#1 T-Cell Therapy Webinar
- 2020 June 10 - ACD Re-Broadcast: Viral Pathogenesis Research: Molecular Detection with RNAscope
- 2020 May 26 - Visualize Splice Variants, Short Targets, and Point Mutations at Single Cell Resolution
- 2020 May 21 - Tissue-based expression analysis by ACD’s in situ hybridization assay services — Simultaneous ISH/IHC Co-Detection of RNA and Protein Targets
- 2020 June 26 - Rebroadcast: Preview of the new RNA ISH Assay to Detect miRNAs, siRNAs and ASOs
- 2020 May - ACD Spatial Genomics Seminar Series
- 2020 May 12 - A Spatial Genomics Webinar - ACD & NanoString
- Visualize Gene Expression with Morphological and Spatial Resolution using RNAscopeTM Chromogenic Assay
- Tissue-Based RNA Detection with ACD’s Pharma Assay Services: Quantitative Image Analysis From Histopathology Experts
- 2020 Apr 14 - Resolving Cell Subtype Specialization with scRNA-seq and RNAscope
- Transcriptional and Spatial Resolution of Cell Types in the Mammalian Habenula
- Tissue-based expression analysis by ACD’s in situ hybridization assay service — high resolution image data in as little as 4 weeks
- Spatial Genomics Seminar by ACD & NanoString
- RNAscope™ Technology Introduction: How to get started as a new user
- 2020 Feb 25 Getting Started and Troubleshooting Tips for BaseScope™ Assays
- Genome-wide Study Reveals a Pseudogene-Associated Recurrent Gene Fusion in Prostate Cancer
- 2020 Jan 28: Visualize your research breakthroughs with RNAscope™ Multiplex Fluorescent assays
- 2019 Year in Review: Key Publications Utilizing the RNAscope™ Technology
- Examination of Microglia at Single Cell Resolution in Health and Disease
- Becoming an Expert: Tips & Tricks for Running the RNAscope® Assay
- RNAscope/BaseScope Probe Design Overview Webinar
- 2019 Nov 12: Single-Cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-Related Macular Degeneration
- Pancreatic Cancer Therapeutic Target Visualized Using In Situ Hybridization
- Molecularly Guided High Plex Spatial Profiling in the Tumor Microenvironment
- Validating single-cell RNA-seq data using Single-Cell Western blotting and RNAscope® technology
- Mind the Yap! Single-Cell Transcriptomes Reveal Drivers of Intestinal Regeneration
- 2019 May 14: Motor neuron size dependent mechanisms of neuroplasticity following spinal cord injury
- A Cellular and Molecular Phenotyping Pipeline for Target Characterization in FFPE Biobank Samples
- Using tools such as single cell RNAseq and spatial mapping, researchers can determine the presence and distribution of monocytes - and their possible roles - in human autoimmune disease.
- Webinar : Visualize Splice Variants, Short Targets, and Point Mutations at Single Cell Resolution
- Training Webinar : Using CellProfiler to Analyze Your RNAscope® Images
- Sensory Experience Shapes a Growing Brain: Regulation of Sensory-Dependent Circuit Refinement by TWEAK/Fn14 Cytokine Signaling
- New Product Introduction: The RNAscope® HiPlex Assay
- Spatial Mapping of Gene Expression at Single Cell Resolution with the RNAscope® Technology
- Becoming an Expert: Tips and Tricks for Getting Started with RNAscope® Assay
- Becoming an Expert: Tips and Tricks for Running BaseScopeTM Assay
- How to correctly interpret your RNAscope® images
- Tips and Tricks for Running the RNAscope® Multiplex Fluorescent Assay
- Tips and Tricks for Getting Started with the RNAscope® and BaseScope™ Assays
- Cancer Gene Expression Targets Validated Using Novel Histopathology Tools
- Role for NaV1.3 in the developing cerebral cortex
- Using single cell RNAseq and in situ hybridization to determine neuronal composition
- Tips and Tricks for Getting Started with RNAscope® and BaseScope™ Assays
- 2018 Dec 3: Application of a Novel ISH Approach to Elucidate Splice Variants in Schizophrenia
- Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
- The Basescope™ Duplex Product Launch Webinar - 10/30 9AM PT
- The RNAscope® Assay Data Analysis Webinar
- Getting Started and Troubleshooting Tips for RNAscope® and BaseScope™ Assays
- Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
- RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS
- Mind the Transcripts: Visualizing RNA Expression and Variants within the Brain
- Elucidation of stem cell dynamics via Rspondin3 regulation using multiplex RNA in situ hybridization techniques
- Understanding heterogeneity in cancer through high-resolution imaging
- RNA in situ Hybridization: The Next Generation of Immunohistochemistry?
- BaseScope™ LS and VS Automated Assays Webinar
- Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
- Tips for Getting Started with RNAscope® and BaseScope™ Assays
- Webinar: Studying the Genetic Basis of Neurodevelopmental Channelopathies
- Tips, Tricks & Troubleshooting RNAscope® and BaseScope™ Assays
- Genetic Variant Detection in Cancer: Using RNAscope® ISH to Track Tumor Evolution
- CAR-T Cell Target Selection Using Innovative in Situ Hybridization Technology
- Getting Started and Troubleshooting Tips for RNAscope® and BaseScope™ Assays
- Combined immunohistochemistry and RNA in situ hybridization to elucidate tumor molecular heterogeneity in prostate cancer
- Characterization of immune checkpoint expression & infiltrating immune cells in TME by RNAscope ISH
- RNAscope® Assay Data Analysis Webinar
- Tips & Tricks to Get Publication Quality RNA ISH Data for Neuroscience Applications
- Product Introduction: RNAscope® VS Universal HRP, AP and Duplex Assays
- Get the most out of your RNAscope® Multiplex Fluorescent assays
- Optimize Your RNAscope® Assay Data Analysis
- Tips, Tricks & Troubleshooting to Get Publication Quality RNA ISH Data
- RNAscope® Assay Data Analysis Webinar
- BaseScope™ Assay: Recent Applications & FAQs
- Introduction to RNAscope® Probes
- New Product Introduction: RNAscope® Multiplex Fluorescent v2 Assay
- New Product Introduction: RNAscope® Multiplex Fluorescent v2 Assay
- Introduction to the RNAscope® Assay
- Visualize your research breakthroughs with RNAscope® and BaseScope™ assays
- Steps for Success with the RNAscope® Multiplex Fluorescent Assay
- RNAscope® Assay Data Interpretation
- Tips for Getting Started with the RNAscope® Multiplex Fluorescent Manual Assay
- Elucidating tumor heterogeneity in prostate cancer by combined IHC & RNA ISH
- New product introduction - BaseScope™ Assay, a new and innovative RNA ISH platform for the detection of short targets and exon junctions.
- New product introduction - BaseScope™ Assay, a new and innovative RNA ISH platform for the detection of short targets and exon junctions.
- New Product Introduction: RNAscope® LS Multiplex Fluorescent Assay
- Tips for Getting Started with the RNAscope® Multiplex Fluorescent Manual Assay
- Advanced Cell Diagnostics’ Pharma Assay Services – Expert capabilities in RNAscope ISH assays and analysis for detection and quantification of mRNA, transcript isoforms and point mutations.
- RNA-ISH for long non-coding RNAs (lncRNAs) - novel game changers in pathology
- Steps for Success with RNAscope® 2.5 VS on the Discovery Ultra and Discovery XT Automated Assays
- 2016 June 21st: Steps for Success with RNAscope® Assays & New Product Development
- Current State-of-the-Art Tissue-Based RNA Analysis with RNAscope Technology & Quantitative Analysis with the HALO Image Analysis Platform
- 2016 May17th: Become an RNAscope® Expert: RNAscope® Data Interpretation Webinar
- 2016 April 26: RNAscope® New Product Development - RNAscope® 2.5 HD Duplex Assay
- 2016 April 26: RNAscope® New Product Development - RNAscope® 2.5 HD Duplex Assay
- 2016 March 15th: New RNAscope® LS Duplex Assay
- 2016 Feb 26th: Become an RNAscope® Expert with the RNAscope® Multiplex Fluorescent assay
- 2016 Jan 28th: PTEN mRNA detection by chromogenic RNA in situ technologies: A reliable alternative to PTEN immunohistochemistry
- Steps for Success with the RNAscope® Assay & New Product Development
- Dec 17th, 2015: Steps for Success with RNAscope® Assay and New Product Development
- 2014 Sept 11th: Whole-Mount Zebrafish Embryos - RNAscope® for High-Resolution Detection of Transcripts with Localization of Proteins
- RNAscope® Technology: A Novel RNA in situ Hybridization Research Platform
- RNAscope® New Product Development - learn about RNAscope 2.5 product line
- RNAscope® New Product Development - learn about RNAscope 2.5 product line
- Linking Viral Discovery with Causality
- Dec 17th, 2015: Steps for Success with RNAscope® Assay and New Product Development
- Visualization of exogenous RNA using RNAscope ISH in rat brain to assess viral vector transduction
- Elucidating tumor heterogeneity in prostate cancer by combined IHC & novel RNA ISH
- Steps for success with the RNAscope assay: Basic training for new users
- Steps for success with the RNAscope assay: Basic training for new users
- Steps for success with the RNAscope assay: Basic training for new users
Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.
How to use EZ-Batch Tray
De-paraffinization
De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.
Endogenous Peroxidase Blocking
RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.
Target Retrieval (Boiling)
Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.
Protease Plus (Protease Digestion)
Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.
Target Probe Hybridization
ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target
Amplification (Amp1-Amp6) with Wash Step
RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.
DAB Colormetric Reaction
Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.
Gill's Hematoxylin Counter Stain
Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe the morphology of the sample and identify the localization of the signal.
Tissue Dehydration
The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better tissue morphology and preservation of the signal.
Mounting
The final step in RNAscope after staining requires the use of mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.
How can we help you?
Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

1 (877) 576-3636