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Spatial mapping of gene expression and genetic variants in the tissue context with the RNAscope technology for Stem Cell Research

Nucleic acid-based molecular detection methods have revolutionized detection of stem cell markers, offering several essential advantages such as sensitivity, specificity, and multiplexing capabilities.

Beyond this, RNAscope ISH uniquely offers molecular detection at a single cell resolution coupled with morphological context to enable visualization of these stem cell markers in various tissue types such as organoids, frozen and FFPE tissues.

In this webinar, we will demonstrate how RNAscope can be used for spatially mapping novel cell types and morphological markers in stem cell research

• Learn about the RNAscope, BaseScope and miRNAscope assays that enable detection of a diverse range of targets.
• Broad applications of the RNAscope technology in research areas such as Oncology, Neuroscience, Infectious disease, Developmental biology and more .
• Compatible workflow with IHC/IF allowing detection of RNA and protein simultaneously.
• Multiplexing capabilities enable detection of multiple targets on the same tissues section.

Learn more about ACD’s new Co-detection  Assays which will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

Combining RNA and Protein enables researchers to

• Characterize cell type-specific expression
• Identify origin of secreted proteins
• Visualize cell surface markers with RNA of interest
• Visualize RNA binding proteins and their target RNA
• Dissect regulation of gene expression
• Antibody validation and specificity confirmation 
• Expand research in oncology, neuroscience, developmental biology, infectious diseases and more

COVID-19 pathogenesis is associated with an exaggerated immune response. However, the specific cellular mediators and inflammatory components driving diverse clinical disease outcomes remain poorly understood. In this webinar sponsored by Advanced Cell Diagnostics (ACD), Madhvi Menon will describe the longitudinal immune profiling of hospitalized patients during the peak of the COVID-19 pandemic in the UK, and the key immune signatures that associated with disease severity. Additionally, Jyoti Phatak-Sheldon will describe what researchers can learn about COVID-19 using ACD’s RNAscope in situ hybridization technology with RNA probes specific for SARS-CoV-2.

 

• Immune profiling of COVID-19 patients in the UK
• Immune signatures associated with severe COVID-19
• Changes in immune signatures after recovery
• RNAscope in situ hybridization technology specific for SARS-CoV-2