• Nov
    27
    Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
    Tapan Shah, PhD., Technical Support Scientist
    Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
    Tapan Shah, PhD.
    Technical Support Scientist
    Outline:

    The RNAscope®  Multiplex Fluorescent assays are ideal for single-molecule detection of multiple RNA targets (up to four targets) simultaneously within the same tissue. They are very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells. This webinar will help new or recent users understand the RNAscope®Multiplex Fluorescent manual assay and discuss the tools for getting started. We will also share tips and tricks to perform the RNAscope® Multiplex assays successfully to obtain publication quality in situ RNA images. 

    Who should attend:
    • This webinar will benefit both new and experienced RNAscope® technology users.
    Learning Objectives:
    • How the RNAscope® assays work
    • What you need to get started
    • Tips and tricks for successful sample preparation
    • Examples of staining results & research application specific data
    • FAQs & Troubleshooting tips
    Dates and Registration:
    November
    27
    9:00 AM PST
    12:00 PM EST
    Register Now
  • Dec
    03
    Application of a Novel ISH Approach to Elucidate Splice Variants in Schizophrenia
    Larissa Erben, National Institutes of Heath, Graduate Student Partnership Program
    Mohammed Qutaish, Ph.D., Senior Image Analysis Scientist, Advanced Cell Diagnostics
    Application of a Novel ISH Approach to Elucidate Splice Variants in Schizophrenia
    Larissa Erben
    National Institutes of Heath, Graduate Student Partnership Program
    Mohammed Qutaish, Ph.D.
    Senior Image Analysis Scientist, Advanced Cell Diagnostics
    Outline:

    This webinar will demonstrate how a research team at the National Institutes of Health evaluated a novel in situ hybridization approach and applied it to study splice variants related to schizophrenia.

    The neurotrophic factor neuregulin-1, as well as its neuronal receptor ErbB4, are risk factors for schizophrenia. Distinct ErbB4 isoforms are generated by alternative splicing and the levels of specific receptor isoforms are altered in postmortem brains of patients.

    Because of these splice variants differ functionally, it is important to identify the cells that express distinct isoforms. However, traditional molecular analysis tools such as qRT-PCR and RNA sequencing require the disruption of dissected tissue to isolate RNA. To investigate in different cell types the relative amounts of the four ErbB4 variants in morphologically conserved brain tissue, the research team used the BaseScope in situ hybridization system with specific oligonucleotides targeting single exon/exon boundaries and fluorescence signal amplification.

    This webinar will outline how the researchers first determined the specificity and sensitivity of the BaseScope system and then used it to identify regional and cell-type specific expression of ErbB4 isoforms in the brain. The presentation will also explain how the this team quantified both the BaseScope and RNAscope assay signals using the freeware CellProfiler combined with an in-house analysis pipeline.

    Dates and Registration:
    December
    03
    10am PST
    1pm EST
    Register Now
  • Dec
    18
    Tips and Tricks for Getting Started with RNAscope® and BaseScope™ Assays
    Sonali Job, PhD., Technical Support Scientist
    Tips and Tricks for Getting Started with RNAscope® and BaseScope™ Assays
    Sonali Job, PhD.
    Technical Support Scientist
    Outline:

    RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS.

    In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.

    Please bring your RNAscope® technology questions to the webinar and our team will answer them live by chat.

     

    Who should attend:
    • This webinar will benefit both new and experienced RNAscope® technology users.
    Learning Objectives:
    • How RNAscope® and BaseScope™ assays work
    • What you need to get started
    • Tips and tricks for successful sample preparation
    • Examples of staining results & research application specific data
    • FAQs & Troubleshooting tips
    Dates and Registration:
    December
    18
    9:00 am PST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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