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In addition to commonly studied messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), multiple types of RNAs have become evident over recent decades, including microRNA (miRNA), long non-protein coding RNA (lncRNA) and circular RNA (circRNA). However, the functions of these RNA types within our cells are not yet fully understood.



With the development of new in situ detection technologies, RNA in situ hybridization has now been enrolled in diagnostics applications, enabling the visualization of RNA localization in tissues and cells to better address their roles in the tissue microenvironment and in molecular pathways.



Join this webinar, with Dr. Boye Schnack Nielsen, Manager of Molecular Histology at Bioneer, to learn more about:

• Our understanding of different RNA types
• RNA in situ hybridization
• Combined RNA and protein staining

• Introduction to the RNAscope/BaseScope/miRNAscope/DNAscope design workflow. 
• Featured applications of RNAscope/BaseScope/miRNAscope/DNAscope probes including detection of RNA, mRNA, pre-RNA, mature microRNA, ASO, siRNA, TCR/BCR, Knockout validation, splice variants, DNA copy number and structural variations. 
• Frequently asked questions - Applications (FAQs).

Fluorescent in situ hybridization (ISH) is a common tool to detect and visualize specific DNA sequences in single cells. Current DNA ISH methods are inferior because they detect genes at low resolution due to long DNA probes and they require expensive, high resolution microscopes. This webinar will introduce DNAscopeTM, an innovative DNA ISH technology that employs a new probe design allowing for the precise targeting of small DNA stretches and convenient visualization under standard light microscopes.

• How ‘double Z’ probe design promotes accurate target labeling
• How to detect DNA breaks and copy number variations
• The clinical potential of DNAscope for both pathology and diagnostic applications

Introducing the RNAscope™ HiPlex v2.0 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples.

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex v2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex v2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME)

• Learn about the expanded capabilities and applications of the HiPlex v2 assay
• Understand how the assay enables characterization of immune cell types and activation states
• Study complex spatial relationships among different cell types within the TME