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In this webinar you will learn how to achieve single-molecule detection of several RNA targets simultaneously and perform co-localization studies of any expressed gene set in nearly any tissue type, while enabling additional capabilities including dual ISH-IHC for RNA and protein co-expression. We will discuss combining the sensitivity of Advanced Cell Diagnostic’s RNAscope Multiplex Fluorescent assays detected with PerkinElmer’s Opal technology and the multispectral imaging capability of PerkinElmer’s Vectra and Mantra Multispectral Quantitative Pathology Imaging instruments.

RNA is a key biomarker of the dynamic gene expression changes occurring in cells and tissues. Compared to techniques that analyze bulk tissue, identifying RNA expression at the single cell level within the morphological context provides valuable spatial information on gene expression. In this webinar we will present the advanced single-cell, single-molecule RNA in situ hybridization (ISH) technology RNAscope. We will discuss the technology, how to perform the assay, how to analyze and quantify the results, and provide multiple applications of the technology in both academic and clinical research settings.

In this webinar we will discuss the benefits of both RNAscope^® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.
Please bring your RNAscope^® technology questions to the webinar and our team will answer them live by chat.

This webinar will benefit both new and experienced RNAscope^® technology users.

• How RNAscope^® and BaseScope™ assays work
• What you need to get started
• Tips and tricks for successful sample preparation
• Examples of staining results & research application specific data
• FAQs & Troubleshooting tips

Sequencing of RNA (RNASeq) can provide a trove of useful information for quantification and characterization of the transcriptome. During bulk sequencing, however, spatial and cellular heterogeneity information is lost. Enter single-cell–level analysis, which can provide unprecedented detail on cellular diversity in development, health, and disease. This emerging area of spatial transcriptomics aims to map a single-cell–level molecular atlas of human tissues in situ. However, mapping entire tissue areas at single-cell quantitative resolution presents significant challenges for imaging and data analysis. In this webinar, efforts to develop large-scale in situ transcriptomics will be described, using the mouse brain as a model system for mapping the molecular diversity of neuronal and glial cell types. The experimental workflow includes automated tissue processing, imaging and analysis for large-scale multiplex mapping, and quantification of gene expression across brain regions in situ at single-cell resolution. The technology that makes this high-resolution mapping possible will be described and compared with other current methods.

During the webinar, the speakers will:

• Describe the power and usefulness of in situ transcriptomics
• Discuss the application of this technology to large-scale tissue mapping in the mouse brain
• Explain how in situ transcriptomics (using RNAScope technology) can complement RNASeq and single-cell RNASeq
• Answer audience questions during the live broadcast!

To learn more about products or technologies related to this webinar, go to http://rna.acdbio.com/transcriptomics.

This webinar will last for approximately 60 minutes.