• Feb
    07
    2023 February 7 - ACD Webinar: From Small RNAs to Chromosomes - An Overview of RNAscope™ Technology and Spatial Biology Tools for Detecting Genes with Single-Molecule Sensitivity and Subcellular Resolution
    Eliot T Smith, PhD, Technical Support Scientist, Advanced Cell Diagnostics
    2023 February 7 - ACD Webinar: From Small RNAs to Chromosomes - An Overview of RNAscope™ Technology and Spatial Biology Tools for Detecting Genes with Single-Molecule Sensitivity and Subcellular Resolution
    Eliot T Smith, PhD
    Technical Support Scientist, Advanced Cell Diagnostics
    Outline:

    From Small RNAs to Chromosomes - An Overview of RNAscope™ Technology and Spatial Biology Tools for Detecting Genes with Single-Molecule Sensitivity and Subcellular Resolution 

    RNAscope technology uses a novel and proprietary in situ hybridization (ISH) approach to visualize the spatial biology of genes, ranging from small RNAs to whole chromosomes, with single-molecule sensitivity and subcellular resolution.   This webinar will provide a technical overview of the RNAscope technology and the utilization of our spatial biology product portfolio.

    Learning Objectives:
    • Overview of the RNAscope technology
    • RNAscope assay workflows and applications
    • Guidelines on sample preparation and pretreatment
    • Guidance on assay setup and appropriate controls
    • Troubleshooting tips
    • Examples of RNAscope results
     
    Dates and Registration:
    February
    07
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Mar
    09
    2023 March 9 - ACD Symposium - Spatial Visualization of RNA Therapeutics and Biomarkers with RNAscope™ ISH
    Outline:

    Spatial Visualization of RNA Therapeutics and Biomarkers with RNAscope ISH

    Save the date! ACD scientists and invited speakers will be speaking about the use of RNAscope™ in situ hybridization technology, our new products and applications. We are able to characterize and visualize morphology-based biodistribution and persistence of genetically engineered products, gene expression and efficacy of RNA therapeutics in various research fields. #genetherapies #celltherapy #biomarkers

    Dates and Registration:
    March
    09
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

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Technical Support Webinar Series

Webinars by Research Area

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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