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In this webinar, you will hear from Dr. Lique Coolen and Dr. Aleisha Moore from Kent State University on how they have integrated powerful RNAscope in situ hybridization technology in their research to study the mechanisms underlying spinal cord injury and neuroendocrine dysfunction.



Dr. Lique Coolen will provide an overview of studies in her laboratory that examine the impact of spinal cord injury on the spinal reflex generator for sexual function in the lumbar spinal cord in male rats. Recent evidence of injury-induced changes in transcript levels of neuropeptides and identification of localization of receptors in this spinal generator using RNAscope in situ hybridization will be presented. Furthermore, pharmacological experiments towards treatment development will be introduced.



Dr. Aleisha Moore will present work in her laboratory identifying changes in neuronal circuits controlling fertility that may lead to polycystic ovarian syndrome, the most common cause of infertility in women worldwide. In her talk, Dr. Moore will focus on how she used RNAscope HiPlex technology in a mouse model of PCOS to evaluate changes in gene expression patterns within complicated and heterogenous hypothalamic neuronal circuits that may underlie the pathogenesis of PCOS.

Key learning objectives in Dr. Coolens talk:

• Learn about impacts of spinal cord injury on urogenital function.
• Become familiar with the use of RNAscope to identify G-protein coupled receptors in spinal cord.

Key learning objectives in Dr. Moore’s talk:

• Learn about the neuroendocrine pathogenesis of PCOS.
• Learn about the current use of RNAscope HiPlex technology to study hypothalamic cell populations.
• Become familiar with Cellprofiler software for automated analysis of RNAscope HiPlex data.

This webinar will present a research project that is using spatial transcriptomics to discern the molecular mechanisms underlying fear memory. 

Kaitlin Sullivan of the University of British Columbia will discuss how she is using multiplexed, fluorescent RNA in situ hybridization (mFISH) based on the RNAscope technology to assess neuronal activity during the creation and recollection of fear memory.

• The use of mFISH to spatially resolve transcriptomically unique neuronal subpopulations.
• How to computationally analyze images and quantify expression at single-cell, single-molecule resolution.
• How to pair mFISH with morphological, circuit, and functional information to get a holistic view of cell types across multiple spatial scales.