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Introducing the RNAscope™ HiPlex v2.0 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples.

The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex v2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex v2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME)

• Learn about the expanded capabilities and applications of the HiPlex v2 assay
• Understand how the assay enables characterization of immune cell types and activation states
• Study complex spatial relationships among different cell types within the TME

RNAscope™ in situ Hybridization Services for Gene Therapy Spatial Biodistribution Assessment

Visual detection and quantification of AAV, lentiviral, and oncolytic viral vector genome biodistribution and transgene expression in tissues with RNAscope and BaseScope™ in situ hybridization. RNAscope in situ hybridization (ISH) has been published as a means of “morphology-based AAV biodistribution analysis.” 

RNAscope in situ assays of vector genome DNA and transgene mRNA combine the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology. Multiplexing enables vector genome and transgene mRNA to be visually assessed and quantified simultaneously in a single slide. With the addition of cell marker probes in multiplex fluorescent RNAscope assays, viral tropism can be measured in any cell population. 
Specific measurement of therapeutic human transgene expression in animal models is often impossible by IHC because of the high levels of homology between transgene and animal ortholog transcripts. BaseScope ISH is another powerful tool that enables specific detection of even single nucleotide differences and can easily differentiate human transgenes from non-human primate and other animal ortholog sequences. 
These assays are enabling more informative and efficient preclinical gene therapy programs and contributing critical data in IND-enabling studies. Please join us to hear how our services are contributing to and advancing gene therapy programs worldwide.

• Monitor AAV vector biodistribution and transgene mRNA expression in conventional FFPE tissues
• Quantitative, single-cell measurement of vector genome and transgene multiplexed with cell-type markers
• Use off-the-shelf probes for WPRE or promoter sequences, or design custom probes in 2 weeks

The human dorsal root ganglion (DRG) is the site of many sensory neurons, including pain-sensing neurons called nociceptors. Therefore, studying the DRG is important for understanding pain responses—especially chronic pain development. Researchers can characterize the DRG by mapping gene expression in its cells. In this webinar brought to you by ACD – a BioTechne brand, Ted Price, Diana Tavares-Ferreira, and Stephanie Shiers will discuss mapping the DRG to identify different neuronal subtypes, thereby aiding target identification and drug discovery with the ultimate goal of developing non-opioid painkillers.

• Transcriptional differences in sensory neuron subtypes in the human DRG
• How differences in neuronal gene expression between species affect drug target development
• How researchers go from target identification to drug development