Microglia are the brain's resident immune cells and play key roles in normal development, maintenance of homeostasis, and disease. Using a combination of single cell sequencing and RNAscope we have uncovered novel populations of microglia in both early post-natal development, injury, aging, and neurodegeneration. We have also found that microglia are uniquely sensitive cells and require great care when performing both scRNA-seq and smFISH/RNAscope to ensure accurate results.
--Spatial Information from RNAscope is key component of single cell studies to both localize populations spatially and validate sequencing results.
--RNAscope verifies artifactual gene expression observed in scRNA-seq when cells isolated under improper conditions
--HiPlex assay enables significantly more accurate localization of specific cell states in situ
