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2020 Apr 14 - Resolving Cell Subtype Specialization with scRNA-seq and RNAscope
Ozgun Gocke, PhD.
Group Leader, Institute for Stroke and Dementia Research (ISD), LMU Munich
Watch Video:
Jyoti Phatak
Associate Scientist, Advanced Cell Diagnostics
Outline:

Discover how a combination of single-cell RNA sequencing (scRNA-seq) and quantitative RNA in situ hybridization (RNAscope) resolves molecular specialization of cell subtypes. Using scRNA-seq and RNAscope, a team from Stanford defined the molecular and anatomical diversity of adult murine striatum spiny projection neurons (SPNs). In this webinar sponsored by ACD, Ozgun Gokce will describe how the team generated a cell type atlas of the adult murine striatum by developing a novel computational pipeline that distinguishes discrete versus continuous cell identities in scRNA-seq data. He will discuss how SPNs in the striatum can be classified into four discrete types that reside in distinct anatomical clusters or are spatially intermingled. Jyoti Phatak from ACD will highlight the cell subtype resolving power of scRNA-seq and RNAscope. Topics to be covered Validation and selection of RNA in situ targets The differences between discrete and continuous cell identities and how to distinguish them in scRNA-seq data Play the On-Demand recording by clicking on the followiong link: https://webinars.the-scientist.com/rnascope-acd

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