2022 Nov 29 - Advanced Multiplexing and Spatial Mapping of RNA Targets using the RNAscope™ HiPlex V2 Assay
Thomas R. Murphy, Ph.D.
Technical Support Scientist, Advanced Cell Diagnostics, a Bio-Techne Brand
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Outline:
Advanced Multiplexing and Spatial Mapping of RNA Targets Using the RNAscope™ HiPlex V2 Assay
RNAscope™ assays are the gold standard for in situ hybridization (ISH), providing spatial visualization of gene expression with unparalleled sensitivity, specificity, and reliability.
Through a unique iterative detection process, the RNAscope HiPlex V2 Assay extends our powerful RNAscope ISH technology to enable simultaneous detection of up to 48 RNA targets with single-molecule resolution. In this webinar we will provide an overview of the RNAscope HiPlex V2 Assay workflow, sample preparation/pretreatment guidelines, imaging, and analysis recommendations, and troubleshooting tips.
Learning Objectives:
- Introduction to RNAscope technology
- Overview of RNAscope HiPlex V2 assay and workflow
- Guidelines for sample preparation and pretreatment
- Examples of RNAscope HiPlex V2 applications and results
- Tips for imaging, analysis, and HiPlex Image Registration software
- Troubleshooting tips
