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2020 Oct 23 - Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples
Shyam Solanki, PhD
Postdoctoral Research Associate, Dept. of Crop and Soil Sciences Washington State University
Watch Video:
Jyoti Phatak-Sheldon, MS
Associate Scientist, Applications, Advanced Cell Diagnostics
Outline:

RNAscope multiplex fluorescent RNA-ISH detection is a powerful and sensitive tool for spatio-temporal RNA species detection in tissues. However, due to plant tissues' morphological and structural characteristics, its implication was not reported until Solanki et al., 2020 (https://doi.org/10.1186/s13007-020-00614-4") presented a modified protocol of tissue preparation and probe hybridization. This modified protocol on the barley leaf tissues can be adapted easily on other plant species and provides a powerful tool for plant researchers and cell biologists. In this webinar, we will discuss a practical approach of carrying out RNAscope assay using a parallel comparison of plant and animal samples to avoid common pitfalls and increase the chances of a successful experiment.

Learning Objectives:
  • Tissue preparation
  • Tissue pretreatments
  • RNAscope V2 assay
  • Sample visualization and image analysis
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