G-protein-coupled Receptors & Ion Channels

Detection of G protein-coupled receptors (GPCRs) - 
Detection of mRNA in the nervous system when no (reliable) antibodies are available 

IHC is a well-established method for a broad range of applications from discovery to diagnostic and prognostic testing. However, raising antibodies to G-protein coupled receptors (GPCRs) can be challenging due to difficulties obtaining suitable antigen accessibility amongst other reasons as GPCRs are transmembrane proteins that can be expressed at low levels and tend to be unstable when purified.. Ion channels, another class of membrane proteins, also constitute a challenging class of targets for antibody discovery since they must remain membrane-associated to maintain their native conformation. The RNAscope technology is an ideal method to visualize these targets within their morphological context in the central and peripheral nervous systems. Examples for the chromogenic and fluorescent detection of GPCRs and ion channels can be found in our image gallery.

Watch our Video: "Detection of G-protein-coupled receptors (GPCRs) in the nervous system using the RNAscope in situ hybridization assay"


  • Hutchings C, Koglin M, Marshall F. Therapeutic antibodies directed at G protein-coupled receptors. mAbs 2010; 2(6), 594-606.

Application note

Learn more in our RNAscope Neuroscience Application Note - Detection of RNA in the central and peripheral nervous system using the RNAscope in situ hybridization assay

Cellular localization of RNA expression in central and peripheral nervous system using RNAscope Technology

Fill out the form below to download the application note:

Neuroscience Gene List

(click on gene name to access corresponding RNAscope probes or download Probe List "RNAscope Probes for Neuroscience Research".)




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