RNAscope™ Multiomic LS Fluorescent Assay
Visualize cell phenotypes and function with single-section spatial multiomics
The RNAscope™ Multiomic LS assay enables highly sensitive and specific detection of both RNA and proteins in the same tissue section for a true multiomic spatial assay. Proven class-leading single-molecule RNAscope technology is leveraged to enable simultaneous detection of up to 6 total proteins and RNA targets with single cell resolution.
The RNAscope™ Multiomic LS assay is designed as an automated assay for the Leica Biosystems’ BOND RX Research Advanced Staining System for high throughput multiomic spatial analysis.
Choose Your Protease-Free Multiomic Workflow
- Protease-free preserves antigne integrity for wide antibody compatibility and maintenance of tissue morphology.
- Use a sequential co-detection workflow by performing RNAscope detection and following with your usual IHC. OR use the fill RNAscope Multiomic workflow for more protein-plex and minimal optimization by selecting from pre-qualified RNAscope antibodies.
Note: Kit also includes protease to support your ongoing projects.
Fast and flexible assay design:
- Up to 6 total plex.
- RNAscope probes let you detect any RNA transcripts. Any gene. Any species.
- Accelerate multiomic assay design at higher plex by selecting from RNAscope antibodies. Pre-qualified RNAscope primary and secondary antibodies minimize optimization time.
Gold-standard RNAscope detection:
- Proven performance for FFPE samples and high to low expressing target genes.
- Backed by 12K publications.
- Over 70K probes visualized.
- Same-slide H&E staining.
Protease-Free Workflow Flexibility
With protease-free technology, detect RNA or RNA and protein together using a workflow that suits your research best.
RNA ISH Detection
For unparalleled sensitivity and specificity.
Detect up to 6 RNA targets at the same time.
Protease-free reagents maintain tissue morphology for precise spatial analysis.
Kit includes both protease-free and protease reagents for maximum compatibility.
RNA & Protein Sequential Multiomic Workflow
Easily perform RNA and protein detection sequentially.
First, perform RNAscope detection with protease-free reagents to preserve antigen integrity.
Then simply follow with standard IHC or IF detection using your own primary and secondary antibodies.
Full RNAscope Multiomic Workflow
For increased protein plex, include RNAscope antibodies for a complete RNA and protein assay.
Select from pre-qualified RNAscope primary antibodies against popular targets.
For even more flexibility, use your own primaries with RNAscope secondary antibodies AND RNAscope primary antibodies.
For a total of 6 RNA and protein targets.
Full Multiomics with RNAscope Antibodies. Easy as 1, 2, 3!
For More advanced multiomic capability, pre-conjugated and qualified primary and secondary antibodies are available. These ensure sensitivity and specificity minimizing laborious assay optimization. Select up to 6 RNA targets for an all-RNA assay or combine both protein and RNA targets in 3 easy steps:
| Step | RNAscope channel assignment | ||||||
|---|---|---|---|---|---|---|---|
| C1 | C2 | C3 | C4 | C5 | C6 | ||
| 1 | Select your own primary antibodies and use with conjugated secondaries | User provided primary antibody-rabbit + RNAscope anti-rabbit-C1 | User provided primary antibody-mouse + RNAscope anti-mouse-C2 | ||||
| 2 | Select conjugated primaries from panel | RNAscope Ab C3 channel | RNAscope Ab C4 channel | RNAscope Ab C5 channel | RNAscope Ab C6 channel | ||
| 3 | Select probes in any open channel | RNAscope C1 probe | RNAscope C2 probe | RNAscope C3 probe | RNAscope C4 probe | RNAscope C5 probe | RNAscope C6 probe |
Technology Overview
The RNAscope Multiomic LS Assay allows simultaneous detection of up to six RNA or protein targets within a single section.
For RNA detection, gene-specific target probes are used, each assigned to different probe channels (- C1 through C6). The lower part of the double Z probe is designed to hybridize to its specific RNA target. The upper part of the double Z probe is specific to a particular probe channel. Channel-specific amplification trees, consisting of preamplifiers and amplifiers, are built. For protein detection, antibodies with conjugated oligonucleotides, analogous to the upper part of the double Z probe, are used and channel-specific amplification trees are then built upon these oligonucleotides.
Signal detection for each channel is performed sequentially with the addition of the final HRP-conjugated oligo and deposition of tyramide linked fluorescent dyes. The combination of ACD patented signal amplification system and the sequential tyramide deposition provides a significant boost in the signal while suppressing background noise, resulting in a highly sensitive and specific assay for both RNA and protein detection.
Visualize RNAs with RNAscope Multiplex v2 detection technology. RNA-specific target probes using proprietary double ZZ design are amplified using RNAscope technology followed by fluorescent TSA detection.
Visualize proteins using ready-to use pre-conjugated primary antibodies or your own unconjugated primary antibodies together with available pre-qualified secondary antibodies. Antibodies are detected using RNAscope amplification technology followed by fluorescent TSA detection.
Fluorophore Selection
The RNAscope Multiomic LS assay supports detection of up to 6 targets simultaneously. The following are the recommended TSA fluorophores to maximize sensitivity and minimize spectral overlap. If using other TSA fluorophores, select the best combination for your imaging system.
| Tocris TSA Flurophore | Tocris Catalog number | 3-plex |
|---|---|---|
TSA Vivid Fluorophore 520 | 323271 | ✓ |
TSA Vivid Fluorophore 570 | 323272 | ✓ |
TSA Vivid Fluorophore 650 | 323273 | ✓ |
| Opal TSA Fluorophore | Akoya Biosciences Catalog number | 3-plex | 4-plex | 5- or 6-plex |
|---|---|---|---|---|
Opal 480 Reagent Pack | FP1500001KT |
|
| ✓ |
Opal 520 Reagent Pack | FP1487001KT | ✓ | ✓ | ✓ |
Opal 570 Reagent Pack | FP1488001KT | ✓ | ✓ | ✓ |
Opal 620 Reagent Pack | FP1495001KT |
|
| ✓ |
Opal 690 Reagent Pack | FP1497001KT | ✓ | ✓ | ✓ |
Opal Polaris 780 Reagent Pack Antibody Diluent/Block (needed when using Opal Polaris 780) | FP1501001KT and ARD1001EA |
| ✓ | ✓ |
Ordering Information
| Assay components | Catalog number | Number of assays |
|---|---|---|
RNAscope™ Multiomic CORE & C1-C3 Channel Kit | 323155 323460 | 60 20 |
RNAscope™ Multiomic CORE & C1-C6 Channel Kit | 323175 323465 | 60 20 |
RNAscope™ Multiomic C4 Channel Reagents | 322950 323445 | 60 20 |
RNAscope™ Multiomic C5 Channel Reagents | 322955 323450 | 60 20 |
RNAscope™ Multiomic C6 Channel Reagents | 322960 323455 | 60 20 |
| Human TIL Panel Primary Antibodies | Catalog number | Number of assays |
|---|---|---|
RNAscope™ Ab Hs CD4-C3 | 322949 | 20 |
RNAscope™ Ab Hs CD8-C4 | 322951 | 20 |
RNAscope™ Ab Hs PanCK-C5 | 322952 | 20 |
RNAscope™ Ab Hs FoxP3-C6 | 322953 | 20 |
RNAscope™ Multiomic Human TIL Antibody Panel Pack includes CD4, CD8, PanCK and FoxP3 antibodies | 323185 | 20 |
Neuro Panel Primary Antibodies Cross reacts against mouse, human, and non-human primate targets. | Catalog number | Number of assays |
|---|---|---|
RNAscope™ Ab NeuN-C3 (Coming Soon) | AB0018-C3 | 20 |
RNAscope™ Ab GFAP-C4 (Coming Soon) | AB0028-C4 | 20 |
RNAscope™ Ab IBA-1-C5 (Coming Soon) | AB0038-C5 | 20 |
RNAscope™ Multiomic Neuronal Antibody Panel Pack includes NeuN, GFAP and IBA-1 antibodies | 321780 | 20 |
| RNAscope Secondary Antibodies | Catalog number | Number of assays |
|---|---|---|
RNAscope™ anti-rabbit-C1 | 322954 | 60 |
RNAscope™ anti-mouse-C2 | 322956 | 60 |
| Control Probes | Catalog number | Number of assays |
|---|---|---|
RNAscope™ Multiomic LS 6-ples Positive Control Probe-Hs RTU mixture of 6 probes targeting human housekeeping genes in each of 6 separate channels, Hs-POLR2A-C1, Hs-PPIB-C2, Hs-UBC-C3, Hs-HPRT1-C4, Hs-ACTB-C5, Hs-TUBB-C6 | 323198 | 30 |
RNAscope™ Multiomic LS Assay Positive Control Probe - Mm RTU mixture of 6 probes targeting mouse housekeeping genes in each of 6 separate channels, Mm-POLR2A-C1, Mm-PPIB-C2, Mm-UBC-C3, Mm-HPRT1-C4, Mm-ACTB-C5, Mm-TUBB-C6 | 322988 | 30 |
RNAscope™ Multiomic LS 6-plex Negative Control Probe | 323208 | 30 |
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