Coming Soon! RNAscope™ Multiomic LS Fluorescent Assay

Expression of 6 RNA targets in fresh frozen mouse hippocampal tissue. RNA targets are GAD1, SLC17A7, AIF1, MBP, AQP4, and STY2.

Expression of 6 RNA targets in fresh frozen mouse hippocampal tissue. RNA targets are GAD1, SLC17A7, AIF1, MBP, AQP4, and STY2.

Expression of 6 RNA targets in fresh frozen mouse hippocampal tissue. RNA targets are GAD1, SLC17A7, AIF1, MBP, AQP4, and STY2.

Expression of 6 RNA targets in fresh frozen mouse hippocampal tissue. RNA targets are GAD1, SLC17A7, AIF1, MBP, AQP4, and STY2.

Human breast cancer stained for 2 protein targets (CD68 and CD163) and 4 RNA targets (IL10, IL-1B, TNFα, and CXCL10).

Human breast cancer stained for 2 protein targets (CD68 and CD163) and 4 RNA targets (IL10, IL-1B, TNFα, and CXCL10).

Human cervical cancer tissue stained for stained for 4 protein targets (CD4, CD8, FoxP3, and PanCK) and 2 RNA targets (IFNG and GZMB).

Human cervical cancer tissue stained for stained for 4 protein targets (CD4, CD8, FoxP3, and PanCK) and 2 RNA targets (IFNG and GZMB).

Visualize cell phenotypes and function with single-section spatial multiomics.

The RNAscope™ Multiomic LS assay enables highly sensitive and specific detection of both RNA and proteins in the same tissue section for a true multiomic spatial assay. Proven class-leading single-molecule RNAscope technology is leveraged to enable simultaneous detection of up to 6 total  proteins and RNA targets with single cell resolution.

The RNAscope™ Multiomic LS assay is designed as an automated assay for the Leica Biosystems’ BOND RX Research Advanced Staining System for high throughput multiomic spatial analysis.

Optimized multiomic workflow:

  • Robust RNAscope Multiplex TSA chemistry for both RNA and protein detection
  • Protease-free workflow preserves antigen integrity and tissue morphology
  • Same-slide H&E staining

Gold-standard RNAscope detection:

  • Proven performance for FFPE samples and high to low expressing target genes.
  • Backed by 10K publications.
  • Over 50K probes visualized.

Fast and flexible assay design:

  • Unlimited RNA targets. Any gene. Any species.
  • Easily add up to 2 of your own primary antibodies.
  • Expand protein plex up to 6 with ready-to-use antibodies to immune markers.

 

Fast and Flexible Assay Design

Oncology and immuno-oncology

Combine up to 4 pre-conjugated antibodies with your own primary antibodies and available conjugated secondaries along with RNA probes to interrogate the tumor microenvironment.

fast_flexible_assay
Mix and match for unlimited flexibility

Select up to 6 RNA targets for an all-RNA assay or combine both protein and RNA targets in 3 easy steps:

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1

Start with up to 2 of your own primary antibodies raised in mouse and rabbit and use with available conjugated secondaries.

2

Add up to 4 pre-conjugated antibodies to common human immuno-oncology targets. CD 4 | CD 8 | FoxP3 | PanCK

3

Add RNA markers in open channels.

Technology Overview

RNAscope Multiomic LS Assay allows simultaneous detection of up to six RNA or protein targets within a single section.

For RNA detection, gene-specific target probes are used, each assigned to different probe channels (- C1 through C6). The lower part of the double Z probe is designed to hybridize to its specific RNA target. The upper part of the double Z probe is specific to a particular probe channel. Channel-specific amplification trees consisting of preamplifiers and amplifiers, are the built.

For protein detection, antibodies with conjugated oligonucleotides, analogous to the upper part of the double Z probe, are used and channel-specific amplification trees are then built upon this.

Signal detection for each channel is performed sequentially with the addition of the final HRP-conjugated oligo and deposition of tyramide linked fluorescent dyes.

The combination of ACD patented signal amplification system and the sequential tyramide deposition provides a significant boost in the signal while suppressing background noise, resulting in a highly sensitive and specific assay for both RNA and protein detection.

RNAscope Chemistry

Visualize RNAs with RNAscope Multiplex v2 detection technology. RNA-specific target probes using proprietary double ZZ design are amplified using RNAscope technology followed by fluorescent TSA detection.

New Protein Capability

Visualize proteins using ready-to use pre-conjugated primary antibodies or your own unconjugated primary antibodies together with available pre-qualified secondary antibodies. Antibodies are detected using RNAscope amplification technology followed by fluorescent TSA detection.

Pre-Ordering Information

Assay componentsCatalog numberNumber of assays

RNAscope™ Multiomic CORE & C1-C3 Channel Kit

323155

60

RNAscope™ Multiomic CORE & C1-C6 Channel Kit

323155

60

RNAscope Multiomic™ Human T-cell antibody Panel Pack (contains one each of CD4, CD8, PanCK, and Fox P3 antibodies)

323185

20

RNAscope™ Multiomic goat anti-rabbit secondary antibody - C1 Reagents

323125

60

RNAscope™ Multiomic goat anti-mouse secondary antibody - C2 Reagents

323145

60

Available Individual Reagent Kits and AntibodiesCatalog numberNumber of assays

RNAscope™ Multiomic C4 Channel Reagents

322950

60

RNAscope™ Multiomic C5 Channel Reagents

322955

60

RNAscope™ Multiomic C6 Channel Reagents

322960

60

RNAscope™ Multiomic Human CD4 antibody - C3 Reagents

322965

20

RNAscope™ Multiomic Human CD4 antibody - C4 Reagents

322985

20

RNAscope™ Multiomic Human PanCK antibody - C5 Reagents

322990

20

RNAscope™ Multiomic Human FoxP3 primary antibody - C6 Reagents

322995

20

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