A Powerful Partnership
Immunohistochemistry (IHC) and RNA in situ Hybridization are 2 widely used technologies sharing the unique capacity to analyze a marker at the single cell level while preserving the morphological context. In many differente situations, IHC and ISH are used in conjuction to validate data or provide complementary information.
Antibody Challenges and Validation
Widespread use of IHC in most laboratories, the lack of universally accepted standardization guidelines for antibody production is limiting the confidence of IHC data and support the need of IHC validation methods. With a quick development time, RNA ISH is the ideal alternative method to validate IHC results.
ISH and IHC complementary
Due to targeting different molecules, the use of ISH in combination with IHC will provide complementary information absolutely necessary to understand molecular mechanism like protein secretion in a complex tissue, identification of cell type producing RNA or reveal gene expression regulation mechanisms.
Combination of both technologies in one single protocol has been recently developed by few researchers to analyze both RNA and protein in the same sample.