RNAscope® VS Universal HRP/ AP

The RNAscope® VS Universal HRP/ AP assays are designed as automated assays on the Discovery Ultra Automated IHC/ISH slide staining systems from Roche. The RNAscope® VS Universal HRP/ AP assays are RNA in situ hybridization assays based on ACD’s patented signal amplification and background suppression technology. The assay uses a proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

The RNAscope® VS Universal HRP Reagent Kit (Brown) is a very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope® VS Universal HRP Reagent Kit (Brown) is designed to provide more intense Diaminobenzidine (DAB) staining—ideal when low copy target gene expression is anticipated (1–20 copies per cell). The stained slides are visualized with brightfield microscopes. 

The RNAscope® VS Universal AP Reagent Kit (Red) with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed,  ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope. 

Sample Images
RNAscope<sup>®</sup> 2.5 VS Red - Human Tonsil - PPIB

RNAscope® 2.5 VS Red - Human Tonsil - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Ovary - PPIB

RNAscope® 2.5 VS Red - Human Ovary - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Lung - PPIB

RNAscope® 2.5 VS Red - Human Lung - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Esophogus - PPIB

RNAscope® 2.5 VS Red - Human Esophogus - PPIB

The RNAscope® VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from Ventana Medical System  (ventanadiscovery.com):

CAT #Order CodeProduct Name and Description
760-22406614353001mRNA DAB Detection Kit
760-24808127166001mRNA Sample Prep Kit
760-22206614337001mRNA Probe Amplification Kit
960-761 — 960-785 250 Test Probe Dispenser User-Fillables 1-25

 

The RNAscope® VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from ACD:

  • RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
  • RNAscope®  2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® VS Universal HRP Reagent Kit (Brown)

 

 

The RNAscope® VS Universal AP Reagent Kit (Red) automated workflow requires the following from Ventana Medical System  (ventanadiscovery.com):

CAT #Order CodeProduct Name and Description
760-23407099037001mRNA RED Detection Kit
760-24808127166001mRNA Sample Prep Kit
760-23607095341001mRNA RED Probe Amplification Kit
960-761 — 960-785 250 Test Probe Dispenser User-Fillables 1-25

 

The RNAscope® VS Universal AP Reagent Kit (Red) automated workflow requires the following from ACD:

  • RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
  • RNAscope®  2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® VS Universal AP Reagent Kit (Red)
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