RNAscope™ VS Universal HRP/ AP
The RNAscope VS Universal HRP/AP assays are designed as automated assays on the DISCOVERY ULTRA instrument, an automated IHC/ISH slide staining platform from Roche Tissue Diagnostics. The RNAscope VS Universal HRP/AP assays are RNA in situ hybridization assays based on ACD’s patented signal amplification and background suppression technology. The assay uses a proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH.
The RNAscope VS Universal HRP assay is a very robust assay gives reliable results which are ideal for archiving when used with the mRNA DAB Detection kit from Roche Tissue Diagnostics. It is the standard in molecular pathology diagnostics usable for a wide source of samples and the results are visible under any standard bright field microscope. In addition to the brown detection kits, other products listed below may also be used:
| Available from Roche Tissue Diagnostics | ||
ACD assays | Product name | Part number | Ordering code |
RNAscope VS Universal HRP | mRNA Green Detection kit mRNA DAB Detection kit | 760-278 760-224 | 08952612001 06614353001 |
†Only needed if running mRNA Green, Teal or Purple HRP Detection kits | |||
The RNAscope VS Universal AP Reagent Kit when used with the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed, ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.
The RNAscope VS Universal AP assay may be utilized with the following AP-based detection kit from Roche Tissue Diagnostics. In addition to the detection kit, other products listed below are also required:
Available from Roche Tissue Diagnostics
| ACD assays | Product name | Part number | Ordering code |
| RNAscope VS Universal AP | mRNA RED Detection kit mRNA RED Probe Amplification kit mRNA Sample Prep kit | 760-234 760-236 760-248 | 07099037001 07095341001 08127166001 |
The RNAscope VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from Roche Tissue Diagnostics (www.diagnostics.roche.com):
| CAT # | Order Code | Product Name and Description |
|---|---|---|
| 760-224 | 06614353001 | mRNA DAB Detection Kit |
| 760-248 | 08127166001 | mRNA Sample Prep Kit |
| 760-222 | 06614337001 | mRNA Probe Amplification Kit |
| 960-761 — 960-785 | 250 Test Probe Dispenser User-Fillables 1-25 |
The RNAscope VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from ACD:
- RNAscope 2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
- RNAscope 2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
- RNAscope Control Slides (optional)
- RNAscope VS Universal HRP Reagent Kit (Brown)
The RNAscope VS Universal AP Reagent Kit (Red) automated workflow requires the following from Roche Tissue Diagnostics (www.diagnostics.roche.com):
| CAT # | Order Code | Product Name and Description |
|---|---|---|
| 760-234 | 07099037001 | mRNA RED Detection Kit |
| 760-248 | 08127166001 | mRNA Sample Prep Kit |
| 760-236 | 07095341001 | mRNA RED Probe Amplification Kit |
| 960-761 — 960-785 | 250 Test Probe Dispenser User-Fillables 1-25 |
The RNAscope VS Universal AP Reagent Kit (Red) automated workflow requires the following from ACD:
- RNAscope 2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
- RNAscope 2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
- RNAscope Control Slides (optional)
- RNAscope VS Universal AP Reagent Kit (Red)
