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RNAscope® VS Universal HRP/ AP

RNAscope VS Hs- KRT19 on Human Liver Tumor

RNAscope VS Hs- KRT19 on Human Liver Tumor

RNAscope VS Hs-CDKN2A on Human Ovarian Tumor

RNAscope VS Hs-CDKN2A on Human Ovarian Tumor

The RNAscope VS Universal HRP/AP assays are designed as automated assays on the DISCOVERY ULTRA instrument, an automated IHC/ISH slide staining platform from Roche Tissue Diagnostics.  The RNAscope VS Universal HRP/AP assays are RNA in situ hybridization assays based on ACD’s patented signal amplification and background suppression technology. The assay uses a proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

The RNAscope VS Universal HRP assay is a very robust assay gives reliable results which are ideal for archiving when used with the mRNA DAB Detection kit from Roche Tissue Diagnostics.  It is the standard in molecular pathology diagnostics usable for a wide source of samples and the results are visible under any standard bright field microscope. In addition to the brown detection kits, other products listed below may also be used:

 

Available from Roche Tissue Diagnostics

ACD assays

Product name

Part number

Ordering code

RNAscope VS Universal HRP

mRNA Green Detection kit

mRNA DAB Detection kit
mRNA Teal HRP Detection kit
mRNA Purple HRP Detection kit
mRNA Probe Amplification kit
DISCOVERY Inhibitor†
mRNA Sample Prep kit

760-278

760-224
760-256
760-255
760-222
760-4840†
760-248

08952612001

06614353001
08352941001
08352909001
06614337001
07017944001†
08127166001

†Only needed if running mRNA Green, Teal or Purple HRP Detection kits

 

The RNAscope® VS Universal AP Reagent Kit when used with the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed, ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.

The RNAscope VS Universal AP assay may be utilized with the following AP-based detection kit from Roche Tissue Diagnostics.  In addition to the detection kit, other products listed below are also required:

 

Available from Roche Tissue Diagnostics

ACD assays

Product name

Part number

Ordering code

RNAscope VS Universal AP

mRNA RED Detection kit
mRNA RED Probe Amplification kit
mRNA Sample Prep kit

760-234
760-236
760-248

07099037001
07095341001
08127166001

 

 

The RNAscope® VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from Ventana Medical System  (ventanadiscovery.com):

CAT #Order CodeProduct Name and Description
760-22406614353001mRNA DAB Detection Kit
760-24808127166001mRNA Sample Prep Kit
760-22206614337001mRNA Probe Amplification Kit
960-761 — 960-785 250 Test Probe Dispenser User-Fillables 1-25

 

The RNAscope® VS Universal HRP Reagent Kit (Brown) automated workflow requires the following from ACD:

  • RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
  • RNAscope®  2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® VS Universal HRP Reagent Kit (Brown)

 

 

The RNAscope® VS Universal AP Reagent Kit (Red) automated workflow requires the following from Ventana Medical System  (ventanadiscovery.com):

CAT #Order CodeProduct Name and Description
760-23407099037001mRNA RED Detection Kit
760-24808127166001mRNA Sample Prep Kit
760-23607095341001mRNA RED Probe Amplification Kit
960-761 — 960-785 250 Test Probe Dispenser User-Fillables 1-25

 

The RNAscope® VS Universal AP Reagent Kit (Red) automated workflow requires the following from ACD:

  • RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
  • RNAscope®  2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® VS Universal AP Reagent Kit (Red)
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Advanced Cell Diagnostics

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Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

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Beijing, China, Zip code: 100176

400-966-1796
info_china.ACD@bio-techne.com
For general information: Leon.Li@bio-techne.com
Web: www.acdbio.com/cn

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