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Research Solution: Preclinical safety assessment and animal model studies

Expression of MKi67 in rat duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of MKi67 in rat duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Expression of MKi67 in cynomulgus duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of MKi67 in cynomulgus duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Expression of MKi67 in dog duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of MKi67 in dog duodenum FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in rat kidney FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in rat kidney FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in cynomulgus kidney FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in cynomulgus kidney FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in dog ovary FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.5 LS Reagent Kit-BROWN

Expression of Pecam1 in dog ovary FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.5 LS Reagent Kit-BROWN

Preclinical drug safety assessment in animal models is well-established as a routine part of pharmaceutical drug development to evaluate the potential safety issues associated with novel therapeutic targets and corresponding lead molecules. Immunohistochemistry (IHC) is the method of choice for assessment of targets and toxicity-related biomarkers. However, systematic application of IHC is hindered by inconsistent performance of commercial antibodies, time-consuming antibody development and validation, and general lack of reagents for some animal models. 
The RNAscope® technology enables detection of almost any RNA biomarker with single-molecule sensitivity and high specificity in formalin-fixed paraffin-embedded (FFPE) tissues. It provides a fast, efficient and universal solution to characterize tissue distribution of drug target and biomarker expression in a highly specific and sensitive manner.

 

ACD Drug Safety Assessment Study

ACD conducted a study using 25 FFPE tissues (provided by Pfizer, Inc.) from 3 species (cynomolgus monkey, dog, and rat) to:

- Identify the optimal pretreatment conditions for different tissues in different species
- Identify positive control probe for each tissue
- Detect specific RNA markers (cell-type, proliferation and apoptosis biomarkers)
These results show the utility and efficiency of RNAscope as a platform for target and safety marker assessment in preclinical animal studies.
 

Want to learn more about this study?
- Watch the poster presentation and download the poster
- Download the application review
- Download the appendix to access the complete study data (123 pages with more than 440 data points)

Drug Safety Assessment Poster Presentation

 

STP Poster 2015
"Fully Automated RNA In Situ Hybridization for Proliferation and Apoptosis-related Biomarkers in Preclinical Animal Model Tissue Panels using RNAscope® 2.5 LS Assay"

Watch Video Download Poster
 

Application Note

Application Note "Robust detection of RNA biomarkers for drug safety assessment in preclinical animal models by fully automated RNAscope® 2.5 LS Assay"

Download pdf

Appendix

Appendix "Robust detection of RNA biomarkers for drug safety assessment in preclinical animal models by fully automated RNAscope® 2.5 LS Assay"

Download pdf
 
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