2020 Aug 7 - Characterizing Complex Tissues with Spatial Expression Analysis Using the RNAscope Technology
Christopher Bunker, Ph.D., MBA
Senior Director, Business Development, Advanced Cell Diagnostics
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Outline:

RNAscope and BaseScope in situ hybridization (ISH) platforms are utilized widely for high resolution tissue-based target expression analysis in human archival and clinical biopsies, as well as in preclinical animal models. ACD’s RNA ISH assays are clinically validated and enable quantitative, cell-specific expression analysis within the TME.  Advances in RNAscope technologies will be discussed in relation to immuno-oncology research and development:

Learning Objectives:
  • Multiplexed RNA ISH
  • Dual ISH/IHC for target analysis and immune profiling
  • Isoform-specific assays with exon junction probes
  • HiPlex 12-plex RNAscope for spatial transcriptomics
  • CAR-T cell detection in solid tumor tissues
  • TCR clone detection in TIL populations
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