Software solutions for all your research needs
The single-molecule sensitivity and visualization makes quantitative RNA in situ hybridization analysis a cutting edge solution. Although many labs do evaluate target probe signals manually using scoring guidelines, the tasks are time consuming and prone to subjectivity and poor reproducibility. Advanced Cell Diagnostics have partnered with Indica Labs and Leica Biosystems to facilitate and improve quantitative scoring. These advanced image analysis solution brings objective and accurate quantification to RNA in situ hybridization. For the first time, gene expression can be measured quantitatively and interpreted by research pathologists within histopathological context.
You can select from HALO® Software from Indica Labs or Aperio RNA ISH Algorithm by Leica Biosystems for automated image analysis solutions for RNAscope™ Assays.
Which Software is right for me?
Aperio RNA ISH Algorithm | HALO Software | |
|---|---|---|
Developer | Aperio RNA ISH Algorithm by Leica Biosystems | Indica Labs |
Features | Quantitative and semi-quantitative outputs Singleplex, brightfield quantification Duplex, brightfield quantification- Red/Green Count individual cells or clusters Identify subcellular location of signal (nucleus, cytoplasm) Compatible with full Aperio Digital Pathology solution | Quantitative and reproducible RNAscope spot counting Easy to use and learnSingle-plex, and multiplex ISH quantification Brightfield and fluorescent ISH quantification Cell-by-cell expression profiles Automatic generation of H-scores and histograms Rapid whole-slide analysis Characterization of cell populations |
Assay Compatibility | RNAscope™ 2.5 HD Reagent Kit—BROWN RNAscope™ 2.5 HD Reagent Kit—RED RNAscope™ 2.5 HD Duplex Reagent Kit RNAscope™ 2.5 LS Reagent Kit—BROWN RNAscope™ 2.5 LS Reagent Kit—RED RNAscope™ 2.5 VS Reagent Kit—BROWN RNAscope™ 2.5 VS Reagent Kit—RED | RNAscope™ 2.5 HD Reagent Kit—BROWN RNAscope™ 2.5 HD Reagent Kit—RED RNAscope™ 2.5 HD Duplex Reagent Kit RNAscope™ Multiplex Fluorescent Reagent Kit RNAscope™ 2.5 LS Reagent Kit—BROWN RNAscope™ 2.5 LS Reagent Kit—RED RNAscope™ 2.5 VS Reagent Kit—BROWN RNAscope™ 2.5 VS Reagent Kit—RED |
ACD Professional Assay Services | Image analysis service available | Image analysis service available |
Commercial Availability | Available for purchase from Leica Biosystems | Available for purchase from Indica Labs |
License Type | One-time license | Annual or perpetual options available for single-seat or multi-seat licenses |
Aperio RNA ISH Algorithm is: For Research Use Only. Not For Use In Diagnostic Procedures.
