2020 Jul 6 - Knowledge Resource Series #4 COVID & Infectious Disease Webinar
Jyoti Phatak-Sheldon
Associate Scientist - Applications, Advanced Cell Diagnostics
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Outline:

Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the etiology and pathogenesis of viral disease. Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity, and speed.

Beyond this, RNAscope ISH uniquely offers a molecular detection at a single cell resolution coupled with morphological context to enable visualization of the virus in different infected tissues and cell types.

In this webinar, we will demonstrate how RNAscope can be used to detect viral infection and pathogenesis, and enhance our understanding of globally important viruses, such as SARS-CoV-2.

 

 

Learning Objectives:

 

  • Single-cell viral detection with high sensitivity,  specificity and rapid probe design strategy for any gene and any species.
  • Complementary to various techniques such as qPCR, IHC, scRNAseq etc.
  • Multiplexed approach for visualization of immune response and histopathological markers
  • Applications include all components of viral studies viral detection
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