RNA expression of FGF19 gene in Human Liver PDX sample using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of FGF19 gene in Human Liver PDX sample using RNAscope® 2.5 HD Assay Brown

RNA expression of Neat1 gene in Mouse Normal Liver sample using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of Neat1 gene in Mouse Normal Liver sample using RNAscope® 2.5 HD Assay Brown

RNA expression of Gdf2 gene in Mouse Normal Liver sample using RNAscope<sup>®</sup> 2.5 HD Assay Brown

RNA expression of Gdf2 gene in Mouse Normal Liver sample using RNAscope® 2.5 HD Assay Brown

427561-Meg3-tv3__Mouse_Normal Liver_RNAscope 2.5 HD Assay Brown

Detection of Meg3-tv2 gene expression in normal mouse liver with RNAscope® 2.5 HD Assay Brown

RAB30 and CD68 expression in human liver FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2-plex Chromogenic duplex assay

RAB30 and CD68 expression in human liver FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2-plex Chromogenic duplex assay

Expression of Timp1 RNA (brown dots) in mouse liver tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.0 HD Reagent Kit-BROWN

Expression of Timp1 RNA (brown dots) in mouse liver tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

RNAscope® Technology in Liver Research

The liver is a complex organ that performs a diverse set of functions including energy homeostasis, lipid and protein synthesis and drug metabolism. To perform these functions the liver consists of multiple lobules, each of which is composed of concentric layers of hepatocytes to form zones. There is significant gene expression heterogeneity among hepatocytes, thereby requiring an in situ method to localize gene expression to the precise anatomical location in the liver. The highly sensitive RNAscope® in situ hybridization (ISH) technology has been used in numerous publications for liver research.

Liver Zonation

  • Glucagon regulates liver zonation through the Wnt/β-catenin pathway
    While Wnt signaling regulates a portion of the liver zonation genes, the regulation of a majority of these genes remains unknown. Cheng et al. show that glucagon is an important regulator of metabolic zonation in the liver. RNAscope® ISH revealed the effects of glucagon on the expression of periportal and perivenous genes in specific layers of liver zonation.

Liver Fibrosis

  • Assessment of fibrosis and RXFP1 expression in human liver
    Despite the clinical burden and advances in understanding the pathogenesis of liver fibrosis, there are still no approved antifibrotic therapies. McBride et al. sought to identify a small molecule agonist for the relaxin GPCR RXFP1 for liver fibrosis treatment. They used RNAscope® ISH to assess RXFP1 expression and distribution in human liver biopsies from 2 types of liver fibrosis, AIH and NASH.

Gene Therapy in Liver

  • Delivery of hEPO mRNA to hepatocytes by lipid nanoparticles
    mRNA therapy provides transient production of therapeutic proteins without the need for nuclear delivery or risk of insertional mutagenesis. DeRosa et al. used lipid nanoparticles to deliver hEPO and hFIX mRNA, showing that exogenous mRNA-derived protein maintains normal activity, confirmed delivery of the mRNA therapy to hepatocytes and that the mRNA therapy degraded over time.

Steatosis

  • Senescent cells promote hepatic fat accumulation and steatosis
    Non-alcoholic fatty liver disease (NAFLD) is characterized by excess hepatic fat (steatosis) and its incidence increases with age. Ogrodnik et al. show that accumulation of senescent cells promote hepatic fat accumulation and steatosis, and that targeting senescent cells could be a therapeutic to reduce steatosis in NAFLD. RNAscope® ISH demonstrated that a high fat diet increased expression of p16 (a senescence marker) but treatment with an apoptosis-inducer reduced expression of p16.

Regeneration

  • Hepatocyte homeostasis and renewal in the liver
    The cellular source of new hepatocytes in the uninjured adult liver remains an unanswered question. Wang et al. identify a cell population in the liver (Wnt-responsive Axin2+ diploid cells) that serves to renew hepatocytes, characterize its niche and identify the signals that regulate its activity. RNAscope® ISH was used to show in the adult mouse liver where the Wnt-target gene Axin2 is expressed and identify the precise cells producing Wnts.

Cellular Source of Secreted Proteins

  • Expression of the angiokine Bmp2 in liver sinusoidal endothelial cells
    Organ-specific endothelial cells control their tissue microenvironment by producing angiocrine growth factors known as angiokines to stimulate blood vessel growth. Bmp2 has been identified as an organ-specific angiokine derived from liver sinusoidal endothelial cells (LSECs). Koch et al. used RNAscope to show Bmp2 expression in LSECS and confirm deletion of Bmp2 specifically in LSECs to understand Bmp2 signaling in the liver.

Hepatitis Research - Read more publications

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