2021 Sept 14 - Spatial analysis for visualizing small RNA species implicated in disease and development
Anushka Dikshit, Ph.D.
Sr. Scientist, Supervisor, Applications, Genomics, Advanced Cell Diagnostics
Watch Video:
Outline:
The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression within the spatial and morphological tissue context. The proprietary probe design in combination with advanced signal amplification enables robustly high signal-to-noise and allows for the detection of gene transcripts at the single molecule level with single-cell resolution.
Now, this webcast will present the miRNAscope™ Assay, an advanced in situ hybridization platform that allows for highly specific and sensitive visualization of small RNA species expression with single-cell resolution.
Learning Objectives:
- The miRNAscope technology and workflow
- Detecting miRNAs implicated in cancer and distinguishing multiple members from the same family
- Functional information provided by identifying cell-type specific miRNAs in the brain
- Visualizing synthetic oligonucleotides such as small interfering RNAs (siRNAs) and anti-sense oligonucleotides (ASOs) that can target specific genes and regulate their expression
