The RNAscope 2.5 High Definition (HD)—BROWN Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.
RNAscope™ 2.5 HD Assay - BROWN
Sample Images for RNAscope 2.5 HD Assay - BROWN
RNAscope 2.5 HD Reagent Kit—BROWN is the ideal starter kit for first time users. This very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope 2.5 HD - Brown Reagent Kits are designed to provide more intense Diaminobenzidine (DAB) staining when low copy target gene expression is anticipated (1–20 copies per cell). The RNAscope 2.5 HD reagent kits are compatible with the same RNAscope probes used previously with RNAscope 2.5 assays.
The RNAscope 2.5 HD—BROWN Manual Assay Workflow Requires:
- RNAscope Target Probes (Catalog or Made-to-Order channel 1 (C1) Probes)
- RNAscope Control Probes (Select species-specific positive control probes and negative control probes)
- RNAscope Control Slides
- RNAscope 2.5 HD Reagent Kit—BROWN (Pretreatment Kit, Detection kit, and Wash Buffer included)
- Brightfield microscope
- HybEZ™ Hybridization System
