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2021 May 27 Knowledge Resource Webinar Series - Applications for Splice Variants, Short Targets, & Point Mutations
Anushka Dikshit, Ph.D.
Applications Scientist, Genomics, Advanced Cell Diagnostics
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The BaseScope assay is a specialized RNA in situ hybridization (ISH) method which has a unique signal amplification system that allows for highly sensitive and specific detection of single nucleotide point mutations, distinguish between splice variants of a target gene and detect short sequences. Alternative tools such as PCR, sequencing and Northern blot are sensitive and specific but are unable to provide spatial information about target transcript expression. The BaseScope assay has been successfully used to assess target expression in Oncology, Neuroscience, Infectious disease and Developmental Biology research areas.

Learning Objectives:
  • Introduction to the BaseScope technology and workflow
  • Visualizing specific CDR3 sequences on T cells in tumor samples
  • Simultaneous detection of two variants of the same gene
  • Detecting circular RNA and other splice variants implicated in neurological diseases
  • Detecting point mutations in sub-clonal populations within heterogenous tumors
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