siRNA/ASO
miRNAscope™ Applications
siRNA/ASO
Visualize and Quantify Small RNA Delivery and Function.
Synthetic oligonucleotides like siRNAs and ASOs precisely target genes to control their expression. With RNAscope you can visualize small regulatory RNAs (smRNAs) alone, or along with its mRNA targets. Assess oligonucleotide therapy delivery, biodistribution, and interrogate smRNA biomarkers along with cell specific/morphology/functional biomarkers.
- The miRNAscope assay allows visualization of ASOs/ miRNA/ siRNA and other nucleic acid targets between 17-50 nucleotides.
- The RNAscope™ Plus smRNA-RNA Assay enables visualization of one small RNA (ASO, miRNA. siRNA) plus upto three mRNA markers.
Unlock Answers to Critical Questions for Your Oligonucleotide-Based Studies.
(1) S12 Nonclinical Biodistribution Considerations for Gene Therapy Products; Guidance for Industry; U.S. Department of Health and Human Services Food and Drug Administration; Center for Drug Evaluation and Research (CDER); Center for Biologics Evaluation and Research (CBER) May 2023; ICH-Safety
Advance Your Small RNA Therapeutic to Visualize Synthetic and Endogenous RNA.
- Assess in vivo biodistribution – of the ASOs or small RNA and determine cellular uptake, and persistence over time.
- Visualize - 1 small RNA (ASO, miRNA, siRNA) + up to 3 mRNA targets in intact tissues.
- Evaluate expression – of the target gene and cell-specific marker genes with morphological context in heterogeneous tissues.
- Safety and efficacy - screen for potential toxicity and off-target effects.
Schematic Representation of the Assay Design.
miRNAscope™ Assay
The targeting siRNA was detected using the siRNA probe in the siRNA-treated mouse liver tissue samples and not in the vehicle tissue samples using the miRNAscope assay, indicating successful delivery of the siRNA-based therapy. Expression of target mRNA was detected using the RNAscope assay and appeared significantly reduced in the siRNA-treated tissue compared to vehicle-treated tissue suggesting successful knock-down of the target mRNA expression with the siRNA-based therapy.
RNAscope™ Plus small RNA-RNA Assay
Detection of Small RNAs and Target RNAs. The assay leverages the patented core technology that enables signal amplification and background suppression for multiplex fluorescent visualization of one small RNA with up to three mRNAs in formalin-fixed paraffin-embedded (FFPE) and fresh frozen samples.
Recorded Webinar
2020 July 28 - miRNAscope Launch Webinar: Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
Get a deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the new robust RNA in situ hybridization assay that enables the detection of ASOs, miRNAs, siRNAs, and other smaller RNAs (16-50nt) with spatial and morphological context at single cell resolution.
Professional Assay Services
- Unparalleled expertise in RNAscope and BaseScope ISH
- High capacity and high throughput, delivering more than 10,000 slides per year
- Supporting dozens of cell and gene therapy companies for research studies enabling pre-IND submission
- Team of specialists perform quantitative image analysis using HALO™ Software (Indica Labs)
- Board-certified pathologist review
- Actionable results delivered in weeks rather than months
Brochure
Accelerate Gene Therapy Development with RNA and Vector DNA Visualization Tools for Biodistribution and Safety Analysis
Flyer
Accelerate Gene Therapy Development with Powerful Visualization Tools for Biodistribution and Functional Analysis
Biodistribution, safety and efficacy assessment of small RNAs with the new RNAscopeTM Plus small RNA-RNA co-detection Assay
