2021 Mar 30 - ACD Support Webinar: Visualization and Analysis of RNAscope™ Results using QuPath
Adeola Adeyemo, Ph.D.
Technical Support Scientist, Advanced Cell Diagnostics
Watch Video:
Ching-Wei Chang, Ph.D.
Senior Image Analysis Scientist Advanced Cell Diagnostics, a Bio-Techne brand
Outline:
The RNAscope in situ hybridization (ISH) technology is a powerful method for detection of gene expression within the spatial and morphological tissue context at the single molecule level.
This webinar will focus on how to analyze RNAscope, BaseScope™, and miRNAscope™ data quantitatively using QuPath open-source software.
A QuPath Demo will guide users through data analysis of both chromogenic and fluorescent images and in selecting the best method for quantifying their images.
Topics covered during this webinar include nuclear and cellular segmentation, color deconvolution, probe dot detection and quantification, classification of cells, visualization of results and scripting of the workflow for batch analysis.
Learning Objectives:
- Overview of RNAscope technology and general imaging guidelines
- Introduction of QuPath for RNAscope ISH image analysis
- Workflow for quantitation of both chromogenic and fluorescent staining
- Examples of RNAscope, BaseScope, and miRNAscope QuPath analysis
