miRNA
miRNAscope™ Applications
miRNA
MicroRNAs (miRNAs) are endogenous, small non-coding RNAs that can regulate the expression of mRNAs post transcriptionally either via translational repression or mRNA degradation. Dysregulation of miRNA expression is observed in various diseases and altered physiological states. Mature miRNAs are single-stranded and approximately 18-22 nucleotides long and can be extremely challenging to visualize in situ.
The new miRNAscope™ assay can:
- Detect miRNAs implicated in cancer and distinguish between multiple members from the same family
Since miRNAs can efficiently regulate the expression of tumor suppressors as well as oncogenes, several miRNAs have been implicated in cancer initiation and progression.
Figure 1: Visualization of miRNA targets in tumor tissues. A, specific detection of highly conserved and closely related miRNAs from the Let7 family. B, detection of different miRNAs expressed in lung cancer.
- Provide functional information by identifying cell-type specific miRNAs in the brain
miRNAs regulate gene expression in developing neuronal cells and are also implicated in pathogenesis of many neurological disorders, neuroinflammation and immune regulation.
Figure 2: Detection of miRNA targets in brain using the miRNAscope assay. A, a sagittal section of the mouse brain demonstrating expression of miR-124-3p. B, Cell-specific expression of different miRNAs in the brain.
- Detect miRNAs significant in development and tissue homeostasis
- miRNAs expressed in the developing tissues can support development, cellular differentiation, maturation, and survival.
- Detect miRNAs vital in immune cell regulation
- miRNAs act as negative or positive feedback regulators of immune cell activation pathways within the cells.
The miRNAscope assay is also compatible with the in situ hybridization - immunohistochemistry (ISH-IHC) workflow which allows visualization of RNA and protein targets simultaneously. Co-detection of miRNA and protein targets can assist in expanding our understanding of cell-specific expression of pathologically relevant miRNAs.
Recorded Webinar
2020 July 28 - miRNAscope Launch Webinar: Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
Get a deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the new robust RNA in situ hybridization assay that enables the detection of ASOs, miRNAs, siRNAs, and other smaller RNAs (16-50nt) with spatial and morphological context at single cell resolution.
Professional Assay Services
- Unparalleled expertise in RNAscope and BaseScope ISH
- High capacity and high throughput, delivering more than 10,000 slides per year
- Supporting dozens of cell and gene therapy companies for research studies enabling pre-IND submission
- Team of specialists perform quantitative image analysis using HALO™ Software (Indica Labs)
- Board-certified pathologist review
- Actionable results delivered in weeks rather than months
