Read about reseachers who take innovative approaches with RNAscope™ protocols to advance their research. If you would like to try one of these customer-demonstrated applications, please note that they are non-standard applications and therefore, a careful read through of the publications/materials is highly recommended. Our technical support team is here and will make best efforts to support you.
Our ACD team are excited that Theresa Gross-Thebing, Azadeh Paksa and Erez Raz at Münster University, Germany have driven the RNAscope™ technology into a new arena - establishing, optimizing and applying RNAscope™ Assay to whole mount embryonic zebrafish samples. Although RNAscope™ assay may be applied to any gene and any species, appropriate sample preparation is essential.
Contact the ACD support team at support.ACD@bio-techne.com for detailed support for the of RNAscope™ Assay.
Theresa Gross-Thebing and Azadeh Paksa have generously volunteered to answer questions specific to the preparation of whole-mount embryonic samples. Email them using RNAscope4zebrafish@bio-techne.com but please note, the response time may take up to 1 week.
A Tips & Tricks and FAQ section surrounding WISH is under development, please check back with us for updates.
WHAT TO ORDER TO GET STARTED?
- RNAscope™ positive control probe: use zebrafish-specific housekeeping gene for positive control. The Erez Raz Lab used zebrafish myoD gene in channel 2, CAT # 402461-C2 Dr-myod1-C2. Alternatively, we also offer CAT# 427301-C2 Dr-gsc-C2 and CAT# 428601 Dr-odc1 (channel 1)
- RNAscope™ negative control probe: CAT # 310043 Negative Control Probe-DapB
- RNAscope™ Target Probes: ACD currently offers 33 zebrafish-specific probes (www.acdbio.com/probesearch). New or custom target probes can be designed and manufactured within 2 weeks.
- RNAscope™ Assay Reagent Kit: The Erez Raz Lab used CAT # 320850 RNAscope™ Multiplex Fluorescent Reagent Kit, comprise of three separate components that are also available separately:
- CAT # 310091 Wash Buffers
- CAT # 320842 RNAscope™ Pretreatment Kit
- CAT # 320851 RNAscope™ Multiplex Fluorescent Detection Kit
A multispectral microscopy imaging system is required for visualization. You will be able to investigate up to 4 genes simultaneously with this assay. Additionally, ACD offers three chromogenic assays; however those assays have not been applied to whole-mount zebrafish embryonic samples.
