PreRun Checklist
RNAscope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_RNAscope Fluorescent Multiplex |
| CHK 47-005 |
| Chk_RNAscope Multiplex Fluorescent v2 |
| CHK 47-007 |
BaseScope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_BaseScope |
| CHK 47-015 |
LS RNAscope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_LS_RNAscope Duplex Chromogenic Assay |
| CHK 47-001 |
| Chk_LS RNAscope Multiplex Fluorescent v2 |
| CHK 47-006 |
| Chk_LS RNAscope Red or Brown |
| CHK 47-016 |
LS BaseScope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_LS BaseScope |
| CHK 47-013 |
VS RNAscope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_VS RNAscope HRP or AP Singleplex |
| CHK 47-008 |
| Chk_VS RNAscope Duplex |
| CHK 47-011 |
VS BaseScope
| Results | Download File | Doc. NO |
|---|---|---|
| Chk_VS BaseScope |
| CHK 47-012 |
Probe Datasheet
Product Inserts
| Title | File | |
|---|---|---|
| RNAscope Fluorescent Multiplex Reagent Kit Product Insert | 320951-PRI | |
| RNAscope ® 2.5 LS Control Probes Pack | 322171-PRI | |
| RNAscope® 2.5 LS ReagentKit RED Product Insert | 322150-PRI | |
| RNAscope® 2.5 LS ReagentKit BROWN Product Insert | 322100-PRI | |
| RNAscope® VS Universal HRP Reagent Kit | 323200-PRI | |
| RNAscope® VS Universal AP Reagent Kit | 323250-PRI | |
| RNAscope® 2.5 LSx Red Product Insert | 322750-PI | |
| RNAscope® 2.5 HD Brown Product Insert | 322300-PRI | |
| RNAscope® 2.5 HD Red Product Insert | 322350-PRI | |
| RNAscope® 2.5 HD Duplex Product Insert | 322430-PRI | |
| RNAscope® Multiplex Fluorescent v2 Product Insert | 323100-PRI | |
| RNAscope® VS Duplex Product Insert | 323300-PRI | |
| BaseScope™ Duplex Product Insert | 323800-PRI | |
| BaseScope™ v2 Detection Kit Product Insert | 323900-PRI | |
| RNAscope® 2.5 LSx Brown Reagent Kit Product Insert | 322700-PI | |
| miRNAscope™ LS Reagent Kit - RED | PI 324600 | |
| miRNAscope™ HD Reagent Kit – RED | PI 324500 | |
| RNAscope® ISH Probe SARS-CoV-2 | 321984 |
Quick Guide
| Title | File | |
|---|---|---|
| RNAscope® 2.5 RED Quick Guide | 322360-QKG | |
| RNAscope® 2.5 BROWN Quick Guide | 322310-QKG | |
| RNAscope_2 5 Duplex_Quick Guide_Detection_03182016_final | 322500 -QKG | |
| Guideline on How to Quantify RNAscope® Fluorescent Assay Results | SOP 45-006 | |
| RNAscope® 2.5 LSx Reagent Kit (BROWN) Quick Guide | 322700-QKG | |
| RNAscope® 2.5 LSx Reagent Kit-Red Quick Guide | 322750-QKG | |
| Sample Prep. for Cultured Cells w/ Chromogenic Assay | 321232 | |
| QG 324500 miRNAscope™ HD Reagents Quick Guide | QG 324500 | |
| HiPlex UP Quick Guide - 324190 | 324190 |
SDS(EN)
320630 RNAscope VS Accessory Kit
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 320631 RNAscope VS Hematoxylin |
| 320631 |
| SDS 320632 RNAscope VS Blueing |
| 320632 |
322310 2.5 HD Brown
322360 2.5 HD Red
| Results | Download File | Doc. NO |
|---|---|---|
| 322001-SDS-EN RNAscope Target Retrieval (10X) |
| 322001 |
| 322331-SDS-EN RNAscope Protease Plus |
| 322331 |
| 322335-SDS-EN RNAscope Hydrogen Peroxide |
| 322335 |
| 322336-SDS-EN RNAscope Protease IV |
| 322336 |
| 322337-SDS-EN RNAscope Protease III |
| 322337 |
| 322361-SDS-EN RNAscope 2.5 HD AMP 5 - Red |
| 322361 |
| 322362-SDS-EN RNAscope 2.5 HD AMP 6 - Red |
| 322362 |
| SDS 320058-EN RNAscope Wash Buffer |
| 320058 |
| SDS 322311 RNAscope 2.5 HD AMP 1 |
| 322311 |
| SDS 322312 RNAscope 2.5 HD AMP 2 |
| 322312 |
| SDS 322313 RNAscope 2.5 HD AMP 3 |
| 322313 |
| SDS 322314 RNAscope 2.5 HD AMP 4 |
| 322314 |
322500 2.5 HD Duplex
323110 Multiplex Fluorescent v2
324400 RNAscope HiPlex12 Detection Reagents (488, 550, 650, 750) v2
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 324111 RNAscope HiPlex AMP 1 |
| 324111 |
| SDS 324112 RNAscope HiPlex AMP 2 |
| 324112 |
| SDS 324113 RNAscope HiPlex AMP 3 |
| 324113 |
| SDS 324401 RNAscope HiPlex Fluoro T1-T4 v2 |
| 324401 |
| SDS 324402 RNAscope HiPlex Fluoro T5-T8 v2 |
| 324402 |
| SDS 324403 RNAscope HiPlex Fluoro T9-T12 v2 |
| 324403 |
| SDS 324404 RNAscope HiPlex FFPE Reagent |
| 324404 |
| SDS 324420 RNascope HiPlex DAPI |
| 324420 |
324409 RNAscope™ HiPlex12 Reagents Kit (488, 550, 650, 750) v2
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 324399 RNAscope Cleaving Stock Solution |
| 324399 |
324410 RNAscope HiPlex12 Detection Reagents (488, 550, 650) v2
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 324111 RNAscope HiPlex AMP 1 |
| 324111 |
| SDS 324112 RNAscope HiPlex AMP 2 |
| 324112 |
| SDS 324113 RNAscope HiPlex AMP 3 |
| 324113 |
| SDS 324404 RNAscope HiPlex FFPE Reagent |
| 324404 |
| SDS 324420 RNascope HiPlex DAPI |
| 324420 |
| SDS 324411 RNAscope HiPlex Fluoro T1-T3 v2 |
| 324411 |
| SDS 324412 RNAscope HiPlex Fluoro T4-T6 v2 |
| 324412 |
| SDS 324413 RNAscope HiPlex Fluoro T7-T9 v2 |
| 324413 |
| SDS 324414 RNAscope HiPlex Fluoro T10-T12 v2 |
| 324414 |
| SDS 324130 RNAscope Cleaving Stock Solution |
| 324130 |
324419 RNAscope™ HiPlex12 Reagents Kit (488, 550, 650) v2
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 324399 RNAscope Cleaving Stock Solution |
| 324399 |
BaseScope Duplex
| Results | Download File | Doc. NO |
|---|---|---|
| 322001-SDS-EN RNAscope Target Retrieval (10X) |
| 322001 |
| 322331-SDS-EN RNAscope Protease Plus |
| 322331 |
| 322335-SDS-EN RNAscope Hydrogen Peroxide |
| 322335 |
| 322336-SDS-EN RNAscope Protease IV |
| 322336 |
| 322337-SDS-EN RNAscope Protease III |
| 322337 |
| SDS 320058-EN RNAscope Wash Buffer |
| 320058 |
| SDS 322919-EN BaseScope Fast Red B |
| 322919 |
| SDS 323811-EN BaseScope Duplex AMP 1 |
| 323811 |
| SDS 323812-EN BaseScope Duplex AMP 2 |
| 323812 |
| SDS 323813-EN BaseScope Duplex AMP 3 |
| 323813 |
| SDS 323814-EN BaseScope Duplex AMP 4 |
| 323814 |
| SDS 323815-EN BaseScope Duplex AMP 5 |
| 323815 |
| SDS 323816-EN BaseScope Duplex AMP 6 |
| 323816 |
| SDS 323818-EN BaseScope Duplex AMP 8 |
| 323818 |
| SDS 323819-EN BaseScope Duplex AMP 9 |
| 323819 |
| SDS 323820-EN BaseScope Duplex AMP 10 |
| 323820 |
| SDS 323821-EN BaseScope Duplex AMP 11 |
| 323821 |
| SDS 323822-EN BaseScope Duplex AMP 12 |
| 323822 |
| SDS 323823-EN BaseScope Green-A |
| 323823 |
| SDS 323824-EN BaseScope Green-B |
| 323824 |
BaseScope LS
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 322101-EN RNAscope 2.5 LS Hydrogen Peroxide |
| 322101 |
| SDS 322102-EN RNAscope 2.5 LS Protease III |
| 322102 |
| SDS 322103-EN RNAscope 2.5 LS Rinse |
| 322103 |
| SDS 323601-EN BaseScope LS AMP 1 |
| 323601 |
| SDS 323602-EN BaseScope LS AMP 2 |
| 323602 |
| SDS 323603-EN BaseScope LS AMP 3 |
| 323603 |
| SDS 323604-EN BaseScope LS AMP 4 |
| 323604 |
| SDS 323605-EN BaseScope LS AMP 5 |
| 323605 |
| SDS 323606-EN BaseScope LS AMP 6 |
| 323606 |
| SDS 323607-EN BaseScope LS AMP 7 |
| 323607 |
| SDS 323608-EN BaseScope LS AMP 8 |
| 323608 |
BaseScope v2
| Results | Download File | Doc. NO |
|---|---|---|
| 322001-SDS-EN RNAscope Target Retrieval (10X) |
| 322001 |
| 322331-SDS-EN RNAscope Protease Plus |
| 322331 |
| 322335-SDS-EN RNAscope Hydrogen Peroxide |
| 322335 |
| 322337-SDS-EN RNAscope Protease III |
| 322337 |
| SDS 320058-EN RNAscope Wash Buffer |
| 320058 |
| SDS 322918-EN BaseScope Fast Red A |
| 322918 |
| SDS 322919-EN BaseScope Fast Red B |
| 322919 |
| SDS 323911-EN BaseScope v2 AMP 1 |
| 323911 |
| SDS 323912-EN BaseScope v2 AMP 2 |
| 323912 |
| SDS 323913-EN BaseScope v2 AMP 3 |
| 323913 |
| SDS 323914-EN BaseScope v2 AMP 4 |
| 323914 |
| SDS 323915-EN BaseScope v2 AMP 5 |
| 323915 |
| SDS 323916-EN BaseScope v2 AMP 6 |
| 323916 |
| SDS 323917-EN BaseScope v2 AMP 7 |
| 323917 |
| SDS 323918-EN BaseScope v2 AMP 8 |
| 323918 |
DNAscope™ HD Duplex Detection Reagents
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 323747 DNAscope Target Retrieval (10X) |
| 323747 |
| SDS 323748 RNA Removal Solution |
| 323748 |
| SDS 324711 DNAscope HD Duplex AMP 1 |
| 324711 |
| SDS 324712 DNAscope HD Duplex AMP 2 |
| 324712 |
| SDS 324713 DNAscope HD Duplex AMP 3 |
| 324713 |
| SDS 324714 DNAscope HD Duplex AMP 4 |
| 324714 |
| SDS 324715 DNAscope HD Duplex AMP 5 |
| 324715 |
| SDS 324716 DNAscope HD Duplex AMP 6 |
| 324716 |
| SDS 324717 DNAscope HD Duplex AMP 7 |
| 324717 |
| SDS 324718 DNAscope HD Duplex AMP 8 |
| 324718 |
| SDS 324719 DNAscope HD Duplex AMP 9 |
| 324719 |
| SDS 324720 DNAscope HD Duplex AMP 10 |
| 324720 |
| SDS 324721 DNAscope Fast Red A |
| 324721 |
| SDS 324722 DNAscope Fast Red B |
| 324722 |
| SDS 324723 DNAscope Blue A |
| 324723 |
| SDS 324724 DNAscope Blue B |
| 324724 |
Probes
| Results | Download File | Doc. NO |
|---|---|---|
| SOP 05-199 SDS Custom Target Probe 50X Concentrates |
| SOP 05-199 |
| SOP 05-198 SDS Custom Target Probes and Diluents |
| SOP 05-198 |
| SOP 05-232 SDS HiPlex12 Control Probes |
| SOP 05-232 |
RNAscope 2.5 LSx Reagent Kit - BROWN
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 322601 RNAscope 2.5 LSx H2O2 |
| 322601 |
| SDS 322602 RNAscope 2.5 LSx Rinse |
| 322602 |
| SDS 322603 RNAscope 2.5 LSx Hematoxylin |
| 322603 |
| SDS 322606 RNAscope 2.5 LSx Bluing Reagent |
| 322606 |
| SDS 322611 RNAscope 2.5 LSx AMP 1 DAB |
| 322611 |
| SDS 322612 RNAscope 2.5 LSx AMP 2 DAB |
| 322612 |
| SDS 322613 RNAscope 2.5 LSx AMP 3 DAB |
| 322613 |
| SDS 322614 RNAscope 2.5 LSx AMP 4 DAB |
| 322614 |
| SDS 322615 RNAscope 2.5 LSx AMP 5 DAB |
| 322615 |
| SDS 322616 RNAscope 2.5 LSx AMP 6 DAB |
| 322616 |
| SDS 322620 RNAscope 2.5 LSx Protease |
| 322620 |
RNAscope 2.5 LSx Reagent Kit - RED
RNAscope H2O2 & Protease Plus Reagents
| Results | Download File | Doc. NO |
|---|---|---|
| 322331-SDS-EN RNAscope Protease Plus Rev A Effective 10.26.2015 |
| 322331 |
| 322335-SDS-EN RNAscope Hydrogen Peroxide Rev A Effective 10.26.2015 |
| 322335 |
RNAscope® 2.5 LS Duplex
RNAscope® 2.5 LS-Brown
| Results | Download File | Doc. NO |
|---|---|---|
| 322104-SDS-EN RNAscope 2.5 LS AMP 1 |
| 322104 |
| 322105-SDS-EN RNAscopeë 2.5 LS AMP 2 |
| 322105 |
| 322106 SDS-EN RNAscope 2.5 LS AMP 3 |
| 322106 |
| 322107-SDS-EN RNAscope 2.5 LS AMP 4 |
| 322107 |
| 322108-SDS-EN RNAscope 2.5 LS AMP 5 - Brown |
| 322108 |
| 322109-SDS-EN RNAscope 2.5 LS AMP 6 - Brown |
| 322109 |
| SDS 322101-EN RNAscope 2.5 LS Hydrogen Peroxide |
| 322101 |
| SDS 322102-EN RNAscope 2.5 LS Protease III |
| 322102 |
| SDS 322103-EN RNAscope 2.5 LS Rinse |
| 322103 |
RNAscope® 2.5 LS-Red
| Results | Download File | Doc. NO |
|---|---|---|
| 322104-SDS-EN RNAscope 2.5 LS AMP 1 |
| 322104 |
| 322105-SDS-EN RNAscopeë 2.5 LS AMP 2 |
| 322105 |
| 322106 SDS-EN RNAscope 2.5 LS AMP 3 |
| 322106 |
| 322107-SDS-EN RNAscope 2.5 LS AMP 4 |
| 322107 |
| 322151-SDS-EN RNAscope 2.5 LS AMP 5 - Red |
| 322151 |
| SDS 322101-EN RNAscope 2.5 LS Hydrogen Peroxide |
| 322101 |
| SDS 322102-EN RNAscope 2.5 LS Protease III |
| 322102 |
| SDS 322103-EN RNAscope 2.5 LS Rinse |
| 322103 |
RNAscope® LS Multiplex
RNAscope® Wash Buffer Reagents
| Results | Download File | Doc. NO |
|---|---|---|
| SDS 320058 RNAscope® Wash Buffer |
| 320058 |
SDS RNAscope® ISH Probe SARS-CoV-2
| Results | Download File | Doc. NO |
|---|---|---|
| SDS (EN) for RNAscope ISH Probe SARS-CoV-2 |
| 200448 |
SDS for RNAscope® VS Duplex
SDS for RNAscope® VS Universal AP
SDS for RNAscope® VS Universal HRP Assay
SDS miRNAscope HD Assay Red
| Results | Download File | Doc. NO |
|---|---|---|
| miRNAscope HD AMP 1 |
| 324511 |
| miRNAscope HD Amp 2 |
| 324512 |
| miRNAscope HD AMP 3 |
| 324513 |
| miRNAscope HD AMP 4 |
| 324514 |
| miRNAscope HD AMP 5 |
| 324515 |
| miRNAscope HD AMP 6 |
| 324516 |
| miRNAscope Fast Red A |
| 324517 |
| miRNAscope Fast Red B |
| 324518 |
SDS miRNAscope LS Red Assay
| Results | Download File | Doc. NO |
|---|---|---|
| miRNAscope LS AMP 1 |
| 324611 |
| miRNAscope LS AMP 2 |
| 324612 |
| miRNAscope LS AMP 3 |
| 324613 |
| miRNAscope LS AMP 4 |
| 324614 |
| miRNAscope LS AMP 5 |
| 324615 |
| miRNAscope LS AMP 6 |
| 324616 |
Universal Pretreatment Kit
| Results | Download File | Doc. NO |
|---|---|---|
| 322337-SDS-EN RNAscope Protease III |
| 322337 |
| 322335-SDS-EN RNAscope Hydrogen Peroxide |
| 322335 |
| 322331-SDS-EN RNAscope Protease Plus Rev A Effective 10.26.2015 |
| 322331 |
VS BaseScope
| Results | Download File | Doc. NO |
|---|---|---|
| 322218-SDS-EN RNAscope 2 5 VS Pretreat 3 - Protease |
| 322218 |
| SDS 322221-IT RNAscope 2.5 VS Target Retrieval |
| 322221 |
| SDS 323218-EN RNAscope VS Protease |
| 323218 |
| SDS 323711-EN BaseScope VS AMP 1 |
| 323711 |
| SDS 323712-EN BaseScope VS AMP 2 |
| 323712 |
| SDS 323713-EN BaseScope VS AMP 3 |
| 323713 |
| SDS 323714-EN BaseScope VS AMP 4 |
| 323714 |
| SDS 323715-EN BaseScope VS AMP 5 |
| 323715 |
| SDS 323716-EN BaseScope VS AMP 6 |
| 323716 |
| SDS 323717-EN BaseScope VS AMP 7 |
| 323717 |
| SDS 323718-EN BaseScope VS AMP 8 |
| 323718 |
Tech Note
Sample Prep / Pretreatment - Specific
Assay / Ancillary - Related
| Results | Download File | Doc. NO |
|---|---|---|
| TN_Ancillary_LS_RNAscope Multiplex Fluorescent_4-plex |
| 322380-TN |
| TN _Ancillary_RNAscope Multiplex Fluorescent V2_4-plex |
| 323120-TN |
ISH-Immunostain (IHC / IF) - Integrated Codetection workflow (ICW) with Antibody application prior to protease treatment
ISH-Immunostain (IHC / IF) - Sequential workflow of complete ISH assay followed by IHC/IF Antibody and staining
| Results | Download File | Doc. NO |
|---|---|---|
| TN_Ancillary_LS_RNAscope Multiplex Fluorescent_Sequential ISH-IF |
| 322818 |
| TN Ancillary RNAscope Multiplex Fluorescent V2_Sequential ISH-IF |
| 323100-TN |
| TN_Ancillary_LS_RNAscope 2.5 Brown_Sequential ISH-IHC |
| MK 50-019 |
| TN_Ancillary_LS_RNAscope 2.5 Red_Sequential ISH-IHC |
| MK 50-018 |
| TN_Ancillary_RNAscope 2.5 Red_Sequential ISH-IF |
| 322350-TN |
| UM_Ancillary_VS_RNAscope_Sequential ISH-IHC |
| MK 50-015 |
Data Analysis
| Results | Download File | Doc. NO |
|---|---|---|
| TN_Software_QuPath for Data Analysis_RNAscope/BaseScope/miRNAscope |
| MK 51-154 |
User Manuals
Manual (Benchtop) Assay
| Results | Download File | Doc. NO |
|---|---|---|
| UM_RNAscope 2.5 HD Red Assay |
| 322360-USM |
| UM_RNAscope 2.5 HD Brown Assay |
| 322310-USM |
| UM_RNAscope Duplex Assay |
| 322500-USM |
| UM_RNAscope Multiplex Fluorescent v2 Assay |
| 323100-USM |
| UM_BaseScope v2 Red Assay |
| 323900-USM |
| UM_BaseScope Duplex Assay_FFPE |
| 323800-USM |
| UM_HybEZ II Hybridization System |
| 321710-USM |
| UM_RNAscope HiPlex v1 (488, 550, 647N, 750) Assay |
| 324100-USM |
| UM_miRNAscope Red Assay |
| 324510 |
| UM_DNAscope Duplex_FFPE Assay |
| 324700 |
| UM_RNAscope HiPlex v2 (488, 550, 650) Assay |
| 324419 |
| UM_RNAscope HiPlex v2 (488, 550, 650, 750) Assay |
| 324409 |
| UM_RNAscopePlus_smRNA-RNA |
| 322785 |
Leica (Automation)
| Results | Download File | Doc. NO |
|---|---|---|
| UM_LS_RNAscope Duplex Assay |
| 322440-USM |
| UM_LS_RNAscope Brown Assay |
| 322100_USM_BDZ11 |
| UM_LS_RNAscope Red Assay |
| 322150_USM_BDZ11 |
| UM_LS_RNAscope Multiplex Fluorescent v2 Assay |
| 322800-USM |
| UM_LSx_RNAscope Brown Assay |
| 322700-USM |
| UM_LSx_RNAscope Red Assay |
| 322750-USM |
| UM_LS_BaseScope v2 Red Assay |
| 323600-USM |
| UM_LS_miRNAscope Red Assay |
| UM 324600 |
| UM_LS_RNAscopePlus_smRNA-RNA |
| 322786 |
Roche (Automation)
| Results | Download File | Doc. NO |
|---|---|---|
| UM_VS_RNAscope HRP Assay |
| 323200-USM-ULT |
| UM_VS_RNAscope AP Assay |
| 323250-USM-ULT |
| UM_VS_RNAscope Duplex Assay |
| 323300-USM-ULT |
| UM_VS RNAscope HRP_Fluorescent |
| MK 50-014 |
| UM_VS_BaseScope v2 Red Assay |
| 323700-USM-ULT |
Software
| Results | Download File | Doc. NO |
|---|---|---|
| RNAscope® HiPlex Image Registration Software User Manual |
| 300065-USM |
