HPV-Related Cancer Research

HPV RNA Detection using in situ hybridization

HPV expression in human head and neck cancer cancer tissue, RNA in situ hybridization (ISH) using automated RNAscope® Ventana Assay - BROWN

HPV RNA Detection using in situ hybridization

HPV E6/E7 expression in human head and neck cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

HPV RNA Detection using in situ hybridization

HPV E6/E7 expression in human head and neck cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

Evidence for transcriptionally active HPV oncogenes E6/E7 is regarded as the gold standard for presence of clinically relevant high-risk human papillomavirus (HPV), but detection of HPV E6 E7 mRNA can be challenging using conventional techniques. As a causal agent in head and neck squamous cell carcinoma (HNSCC), it is critical that the detection method enable pathologist review of tissue morphology and be of the highest specificity and sensitivity for accurate assessment of within the tissue microenvironment of FFPE specimens. RNAscope® HPV Biomarker Detection Reagents and its proprietary “double Z” oligonucleotide probes specific for each subtype HPV E6/E7 mRNA enable high specificity detection of viral transcripts in routine FFPE tumor biopsies.

RNA ISH Assay Provides Highest Sensitivity and Specificity
RNAscope
® for in situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Current methodologies for HPV testing include PCR-based amplification and DNA in situ Hybridization (ISH). PCR amplification of HPV DNA is more sensitive, but it is less specific than DNA ISH. Published studies (Bishop et. al., 2013, Upko et. al., 2011, and Schache et. al., 2013) indicate that RNAscope®-based ISH assay is more sensitive than DNA ISH in detecting HPV in Oropharyngeal Squamous Cell Carcinoma (OSCC) and results correlate well with p16 immunohistochemistry (IHC) staining (Table 1).  RNAscope® ISH technology is an ideal platform giving the high sensitivity and specificity needed for detection of HPV biomarkers in Head and Neck Squamous Cell Carcinoma (HNSCC) samples.

ASSAY METHODSENSITIVITYSPECIFICITYPPVNPV
p16 IHC97%82%80%97%
HR-HPV DNA ISH94%91%89%95%
Combined p16/HR-HPV DNA ISH94%91%89%95%
DNA qPCR91%87%83%93%
Combined p16/DNA qPCR91%93%91%93%
RNAscope® HR-HPV97%93%91%98%

TABLE 1. Using the current “gold-standard” qPCR as the reference method, RNAscope® Technology demonstrated the best sensitivity and specificity for HPV status determination than existing methods (Schache et al, 2013).
Abbreviations: IHC=immunohistochemistry; ISH=in situ hybridization; NPV=negative predictive value; PPV=positive predictive value; qPCR=quantitative PCR

RNAscope® HPV Application Review

Read more about how to asses transcriptionally active HPV biomarkers in our RNAscope® HPV Application Review.

Complete form to download PDF

RNASCOPE® CATALOG HPV POOLED PROBES

(Click on the name to access corresponding RNAscope® Catalog Probes)

 

 

 

RNASCOPE® CATALOG INDIVIDUAL HPV PROBES

(Click on the name to access corresponding RNAscope® Catalog Probes)

 

 

 

** If your gene of interest is not listed or is listed above but there is no catalog probe targeting your species of choice,  ACD can design and manufacture new probes in 2 weeks.

Request New Probes

 

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com