RNAscope® and Basescope™ Probes

The RNAscope® and BaseScope™ Assays are based on the proprietary RNAscope ® Technology enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity. Learn more about the difference between RNAscope® and BaseScope™ Assays.

Catalog and Made-to-Order Probes

Using proprietary ACD RNAscope® Probe Design Pipeline, we provide double "Z" oligo probes designed to hybridize to your specific RNA target of interest. We can design in situ hybridization RNA probes for virtually ANY gene in ANY genome for visualization in ANY tissue or Cells.

Catalog Target Probes

Previously designed and used by our customers, they can be shipped to you in approx. 2 weeks. Our catalog probes span over 13,000 RNA targets in over 140 species including human, mouse, rat, dog, cow, zebrafish, rabbit, pig, chicken, monkeys and over 100 viruses. Search and select from our over ever-growing catalog of in situ hybridization probe sets.

RNAscope® Made-to-Order Target Probes

New probes for your gene of interest will be designed and manufactured in about 2 weeks.

Probe Exclusivity

Under certain conditions, ACD may agree to grant exclusivity to probes against "proprietary and private" targets for a fee. While ACD still owns the probe design for these targets, we are committed to not market these probes until the probe exclusivity is removed. Please talk to your account executive for further information.

Probe Name Nomenclature
RNAscope® Probe Name Nomenclature
  • Probe
     
    Probe Type
  • Hs
     
    Species
  • FDXR
     
    Gene
  • 5UTR
     
    Specification
  • C2
     
    Amplification Channel
Example 1
LS Probe - Mm-Rspo3

This probe used in Leica Automated System Assay(LS Probe) and is designed to hybridize to Mus musculus (Mm) Rspo3 gene. Its spectral channel is not listed which means Spectral Channel 1 and therefore compatible with any of the four manual asays including RNAscope® 2.5 HD Reagent Kit-BROWN, RNAscope® 2.5 HD Reagent Kit-RED, RNAscope® 2.5 HD Duplex Reagent Kit or RNAscope® Multiplex Fluorescent Reagent Kit.

Example 2
Probe-Hs-GNRHR-5UTR-C2

This is probe is designed to be used with a manual assay (Probe). It targets just the 5' untranslated region (5UTR) of the GNRHR gene from the human sequence(Hs). This probe is compatible with spectral channel 2 (C2), so it can be used in a duplex or multiplex assay.

 

BaseScope™ Probe Name Nomenclature
  • BA
     
    Platform
  • Mm
     
    Species
  • Nrg1
     
    Gene
  • E1E2
     
    Sub-Type
Example
BaseScope™ Probe - BA-Dm-Gapdh1-3zz

This probe is compatible with Manual BaseScope™ assays and is designed to hybridize to Drosophila melanogaster Gapdh1 gene. The 3ZZ specifies the number of probe pairs and indicates this is a short target probe.

Probe Compatibility

Probes
Assay Compatibility

RNAscope® Manual Assays

RNAscope® 2.5 LS

RNAscope® 2.5 VS

BaseScope™ Manual Assay

Probe Name & Naming convention

Diaminobenzidine(DAB)RNAscope Probe
Eg: Probe - Hs-FDXR-C2

2.5 LS RNAscope® Probe
Eg: 2.5 LS Probe - Hs-FDXR

2.5 VS RNAscope® Probe
Eg: 2.5 VS Probe - Hs-FDXR

BaseScope™ Probe
Eg: BA-Mm-Hdgfrp3-E3E4

Probe naming convention

Probe Type-Species-GeneName-Channel

Probe Type-Species-GeneName

Probe Type-Species-GeneName

Platform-Species-Gene-Sub-Type

Spectral Channel Compatibility

Available for Channel 1-4
(C1, C2, & C3)
When Channel No. is missing, the probe is a C1 probe

Channel 1 Only
2.5 LS Probe names often do not contain Channel No..

Channel 1 Only
2.5 VS Probe names often do not contain Channel No..

Channel 1 Only
Probe names often do not contain Channel No..

C1 Probes Assay Compatibility

C1 is spectral channel 1 and is the most abundant probe type.
RNAscope® 2.5 HD Reagent Kit--BROWN
RNAscope® 2.5 HD Reagent Kit--RED 
RNAscope® 2.5 HD Duplex Reagent Kit
RNAscope® Multiplex Fluorescent Reagent Kit
RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® 2.5 LS Assay Reagent Kit--BROWN 
RNAscope® 2.5 LS Assay Reagent Kit--RED

RNAscope® 2.5 VS Assay Reagent Kit--BROWN
RNAscope® 2.5 VS Assay Reagent Kit--RED

BaseScope™ Reagent Kit–RED

C2 Probes Assay Compatibility

C2 is spectral channel 2
RNAscope® 2-Plex Reagent Kit
RNAscope® Multiplex Fluorescent Reagent Kit
RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® Multiplex Fluorescent Reagent Kit v2

Not Available

Not Available

C3 Probes Assay Compatibility

C3 is spectral channel 3
RNAscope® Multiplex Fluorescent Reagent Kit
RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® Multiplex Fluorescent Reagent Kit v2

Not Available

Not Available

C4 Probes Assay Compatibility

C4 is spectral channel 4
RNAscope® Multiplex Fluorescent Reagent Kit v2 and RNAscope® 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2

RNAscope® LS Multiplex Fluorescent Reagent Kit and RNAscope® LS 4-Plex Ancillary Kit for LS Multiplex Fluorescent

Not Available

Not Available

Probe Volume

Probes for 20 slides

Probes for 30 slides

Probes for 30 slides

Probes for 20 slides

Species Designation Probe name contains two letters to indicate species name

Hs-Homo Sapien
Mm-Mus musculus
Rt-Rattus norvegicus
ACD offers >40 species

Hs-Homo Sapien
Mm-Mus musculus
Rt-Rattus norvegicus
ACD offers >40 species

Hs-Homo Sapien
Mm-Mus musculus
Rt-Rattus norvegicus
ACD offers >40 species

Hs-Homo Sapien
Mm-Mus musculus
Rt-Rattus norvegicus

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