RNAscope® and Basescope™ Probes
The RNAscope® and BaseScope™ Assays are based on the proprietary RNAscope ® Technology enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity. Learn more about the difference between RNAscope® and BaseScope™ Assays.
Using proprietary ACD RNAscope® Probe Design Pipeline, we provide double "Z" oligo probes designed to hybridize to your specific RNA target of interest. We can design in situ hybridization RNA probes for virtually ANY gene in ANY genome for visualization in ANY tissue or Cells.
Previously designed and used by our customers, they can be shipped to you in approx. 2 weeks. Our catalog probes span over 13,000 RNA targets in over 140 species including human, mouse, rat, dog, cow, zebrafish, rabbit, pig, chicken, monkeys and over 100 viruses. Search and select from our over ever-growing catalog of in situ hybridization probe sets.
New probes for your gene of interest will be designed and manufactured in about 2 weeks.
Under certain conditions, ACD may agree to grant exclusivity to probes against "proprietary and private" targets for a fee. While ACD still owns the probe design for these targets, we are committed to not market these probes until the probe exclusivity is removed. Please talk to your account executive for further information.
- ProbeProbe Type
- HsSpecies
- FDXRGene
- 5UTRSpecification
- C2Amplification Channel
This probe used in Leica Automated System Assay(LS Probe) and is designed to hybridize to Mus musculus (Mm) Rspo3 gene. Its spectral channel is not listed which means Spectral Channel 1 and therefore compatible with any of the four manual asays including RNAscope® 2.5 HD Reagent Kit-BROWN, RNAscope® 2.5 HD Reagent Kit-RED, RNAscope® 2.5 HD Duplex Reagent Kit or RNAscope® Multiplex Fluorescent Reagent Kit.
This is probe is designed to be used with a manual assay (Probe). It targets just the 5' untranslated region (5UTR) of the GNRHR gene from the human sequence(Hs). This probe is compatible with spectral channel 2 (C2), so it can be used in a duplex or multiplex assay.
- BAPlatform
- MmSpecies
- Nrg1Gene
- E1E2Sub-Type
This probe is compatible with Manual BaseScope™ assays and is designed to hybridize to Drosophila melanogaster Gapdh1 gene. The 3ZZ specifies the number of probe pairs and indicates this is a short target probe.
Probes | RNAscope® Manual Assays | RNAscope® 2.5 LS (Leica Biosystems) | RNAscope® 2.5 VS (Ventana Systems) | BaseScope™ Manual Assay |
|---|---|---|---|---|
Probe Name & Naming convention | Diaminobenzidine(DAB)RNAscope Probe | 2.5 LS RNAscope® Probe | 2.5 VS RNAscope® Probe | BaseScope™ Probe |
Probe naming convention | Probe Type-Species-GeneName-Channel | Probe Type-Species-GeneName | Probe Type-Species-GeneName | Platform-Species-Gene-Sub-Type |
Spectral Channel Compatibility | Available for Channel 1-4 | Available for Channel 1-4 | Available for Channel 1 and 2 Only. | Channel 1 and 2 Only. When Channel No. is missing, the probe is a C1 probe. |
C1 Probes Assay Compatibility | C1 is spectral channel 1 and is the most abundant probe type. RNAscope® 2.5 HD Reagent Kit--RED RNAscope® 2.5 HD Duplex Reagent Kit RNAscope® Fluorescent Multiplex Reagent Kit RNAscope® Multiplex Fluorescent Reagent Kit v2 | RNAscope® 2.5 LS Assay Reagent Kit--BROWN RNAscope® 2.5 LS Assay Reagent Kit--RED RNAscope® 2.5 LS Duplex Reagent Kit RNAscope® LS Multiplex Fluorescent Reagent Kit | RNAscope® VS Universal HRP Reagent Kit RNAscope® VS Universal AP Reagent Kit RNAscope® VS Duplex Reagent Kit | BaseScope™ Reagent Kit v2–RED BaseScope™ Duplex Reagent Kit |
C2 Probes Assay Compatibility | C2 is spectral channel 2 RNAscope® Fluorescent Multiplex Reagent Kit RNAscope® Multiplex Fluorescent Reagent Kit v2 | RNAscope® 2.5 LS Duplex Reagent Kit RNAscope® LS Multiplex Fluorescent Reagent Kit | RNAscope® VS Duplex Reagent Kit | BaseScope™ Duplex Reagent Kit |
C3 Probes Assay Compatibility | C3 is spectral channel 3 RNAscope® Multiplex Fluorescent Reagent Kit v2 | RNAscope® LS Multiplex Fluorescent Reagent Kit | Not Available | Not Available |
C4 Probes Assay Compatibility | C4 is spectral channel 4 | RNAscope® LS 4-Plex Ancillary Kit for LS Multiplex Fluorescent | Not Available | Not Available |
Probe Volume | Probes for 20 slides | Probes for 30 slides | Probes for 30 slides | Probes for 20 slides |
Species Designation Probe name contains two letters to indicate species name | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien |
