Learn more about our New BaseScopeTM assay

RNAscope 2.5 HD Assay - RED

The RNAscope® 2.5 High Definition (HD)—Red Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.

Sample Images
RNAscope<sup>®</sup> 2.5 - Lung Cancer- PDL1

RNAscope® 2.5 - Lung Cancer- PDL1

The RNAscope® 2.5 HD Reagent Kit-RED assay with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.

The RNAscope® 2.5 HD--RED Manual Assay Workflow Requires:

  • RNAscope® Target Probes (Catalog or Made-to-Order C1 Probes)
  • RNAscope® Control Probes (Select species-specific positive control probes and negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® 2.5 HD Reagent Kit—RED (Pretreatment Kit, Detection kit, and Wash Buffer included)
  • Brightfield microscope or multispectral fluorescent imaging system
  • HybEZ™ Hybridization System

Product Retirement:

Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).

Please refer to the FAQ for more information about 2.0 discontinuance.

More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope® New Product Development- learn more about RNAscope 2.5 product line” here

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