- Mar052021 Mar 5 - Webinar: Introducing New RNA-Protein Co-Detection Assays2021 Mar 5 - Webinar: Introducing New RNA-Protein Co-Detection AssaysMadhura NayakProduct Manager, Advanced Cell Diagnostics, a Bio-Techne BrandAnushka Dikshit, Ph.D.Applications Scientist, Advanced Cell DiagnosticsOutline:
Launch Webinar: Introducing ACD's New RNA-Protein Co-Detection Assays
Learn more about ACD’s new Co-detection Assays which will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.
Learning Objectives:Combining RNA and Protein enables researchers to:- Characterize cell type-specific expression
- Identify origin of secreted proteins
- Visualize cell surface markers with RNA of interest
- Visualize RNA binding proteins and their target RNA
- Dissect regulation of gene expression
- Antibody validation and specificity confirmation
- Expand research in oncology, neuroscience, developmental biology, infectious diseases and more
Dates and Registration: - Mar102021 Mar 10 - Webinar: Spatial Expression Analysis Elucidates Mechanisms of circRNAs in Cancer2021 Mar 10 - Webinar: Spatial Expression Analysis Elucidates Mechanisms of circRNAs in CancerLasse Sommer Kristensen, PhDAssociate Professor, Department of Biomedicin, Aarhus University,Anushka Dikshit, Ph.D.Applications Scientist, Advanced Cell DiagnosticsOutline:Circular RNAs (circRNAs) have recently gained substantial attention in the cancer research field where most, including the putative oncogene ciRS-7 (CDR1as), have been proposed to function as competitive endogenous RNAs (ceRNAs) by “sponging” specific microRNAs.
In this webinar, Lasse Sommer Kristensen of Aarhus University will describe his team’s work in studying the role of circRNAs in cancer. His team recently reported the first spatially resolved cellular expression patterns of ciRS-7 in colon cancer. Surprisingly, they found that ciRS-7 is completely absent in the cancer cells, but highly expressed in stromal cells within the tumor microenvironment.
In addition, they proposed a model to explain correlations between circRNA and mRNA expression, which are commonly interpreted as evidence of a ceRNA function, based on different cancer-to-stromal cell ratios among the studied tumor specimens.
Together, these results have wide implications for future circRNA studies and highlight the importance of spatially resolving expression patterns of circRNAs proposed to function as ceRNAs.Learning Objectives:Key points to be discussed in this webinar:- The circular RNA named ciRS-7 is overexpressed in human cancers; however, in many types of cancer it is not expressed in the cancer cells, but abundant in stromal cells within the tumors
- Cancer-to-stromal cell ratios contribute to correlations between circRNAs and microRNA target genes
- Analyses of spatial expression patterns are key to understanding molecular mechanisms of circRNAs in cancer
Dates and Registration: - Mar172021 Mar 17 - Oncofetal ecosystem: space, time and memory2021 Mar 17 - Oncofetal ecosystem: space, time and memoryAnkur Sharma, Ph.D.,Laboratory Head at Harry Perkins Institute of Medical Research and women’s cancer senior fellow/Senior lecturer at Curtin Health Innovation Research Institute (CHIRI), Curtin University, Perth, Australia.Anushka Dikshit, Ph.D.Applications Scientist, Advanced Cell DiagnosticsOutline:
This webcast will describe employing single cell genomics and spatial transcriptomics to discover a previously unexplored oncofetal reprogramming of the tumor ecosystem.
Analysis of ~212,000 cells representing human fetal, hepatocellular carcinoma (HCC), and mouse liver revealed remarkable fetal-like reprogramming of the tumor microenvironment. Specifically, the HCC ecosystem displayed features reminiscent of fetal development, including re-emergence of fetal-associated endothelial cells and fetal-like tumor-associated macrophages.
The speaker will describe a shared immunosuppressive oncofetal ecosystem in fetal liver and HCC and its potential implications for targeting therapeutic interventions in HCC and identifying similar paradigms in other cancers and diseases.
Learning Objectives:- Identification of fetal-associated endothelial cells and macrophages in HCC
- Shared oncofetal ecosystem between human fetal liver and HCC
- The phenomenon of oncofetal reprogramming in the tumor ecosystem
Dates and Registration: - Mar182021 Mar 18 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression Analysis2021 Mar 18 - PAS Webinar: Introducing Professional Assay Services - RNAscope™ ISH Experts in Tissue Expression AnalysisMansi Garg, M.S.Sr. Manager, Project Operations Advanced Cell Diagnostics, a Bio-Techne brandRuby Hsu, Ph.D.,Director, Business Development Advanced Cell Diagnostics, a Bio-Techne brandOutline:This webinar will introduce our RNAscope in situ hybridization (ISH) services, as well as our quantitative image analysis capabilities and quality-controlled tissue bank for target validation.
Bio-Techne’s Professional Assay Services (PAS) is the only CRO specialized in RNAscope, BaseScope™ and miRNAscope™ ISH services. We provide tissue-based and cell specific analysis of targets and biomarkers in as little as 4 weeks.
Please join us to hear how our services are advancing the research and drug development at hundreds of academic and clinical institutions worldwide. Hear examples of studies in immuno-oncology, cell and gene therapy, neuroscience, single-cell RNAseq validation, biomarker validation, and more.Learning Objectives:- Visualize the cellular heterogeneity of tissues and solid tumors with the multiplexing and spatial capabilities of the RNAscope Multiplex Fluorescent assay
- Specific and sensitive biomarker validation to accelerate translation of assays into the clinic
- Morphology-based biodistribution analysis of AAV vector and transgene expression
- CAR-T and TCR-T cell tumor infiltration and activity assays
- Detection of splice variants, point mutations and short target sequences with BaseScope ISH assays
- Co-stain with cell-type markers using our ISH/IHC Co-Detection workflow
- PAS’s quality-controlled tissue bank including solid tumors, diseased and normal human tissues, and TMAs
- RNAscope image analysis offerings for quantitative approaches to scoring
Dates and Registration: - Mar192021 Mar 19 - Webinar: Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples2021 Mar 19 - Webinar: Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue SamplesShyam Solanki, Ph.D.Postdoctoral Research Associate, Dept. of Crop and Soil Sciences Washington State UniversityJyoti Phatak-Sheldon, MSAssociate Scientist, Advanced Cell DiagnosticsOutline:Join us as we discuss how this modified protocol on the plant leaf tissues can be adapted easily on other plant species and provides a powerful tool of RNAscope for RNA visualization.
RNAscope multiplex fluorescent RNA-ISH detection is a powerful and sensitive tool for spatio-temporal RNA species detection in tissues. However, due to plant tissues' morphological and structural characteristics, its implication was not reported until Solanki et al., 2020 (https://doi.org/10.1186/s13007-020-00614-4) presented a modified protocol of tissue preparation and probe hybridization. This modified protocol on the barley leaf tissues can be adapted easily on other plant species and provides a powerful tool for plant researchers and cell biologists.In this webinar, we will discuss a practical approach of carrying out RNAscope assay using a parallel comparison of plant and animal samples to avoid common pitfalls and increase the chances of a successful experiment.Learning Objectives:- Tissue preparation
- Tissue pretreatments
- RNAscope V2 assay
- Sample visualization and image analysis
Dates and Registration: - Mar302021 Mar 30 - ACD Support Webinar: Visualization and Analysis of RNAscope™ Results using QuPath2021 Mar 30 - ACD Support Webinar: Visualization and Analysis of RNAscope™ Results using QuPathAdeola Adeyemo, Ph.D.Technical Support Scientist, Advanced Cell DiagnosticsChing-Wei Chang, Ph.D.Senior Image Analysis Scientist Advanced Cell Diagnostics, a Bio-Techne brandOutline:The RNAscope in situ hybridization (ISH) technology is a powerful method for detection of gene expression within the spatial and morphological tissue context at the single molecule level.
This webinar will focus on how to analyze RNAscope, BaseScope™, and miRNAscope™ data quantitatively using QuPath open-source software.
A QuPath Demo will guide users through data analysis of both chromogenic and fluorescent images and in selecting the best method for quantifying their images.
Topics covered during this webinar include nuclear and cellular segmentation, color deconvolution, probe dot detection and quantification, classification of cells, visualization of results and scripting of the workflow for batch analysis.Learning Objectives:- Overview of RNAscope technology and general imaging guidelines
- Introduction of QuPath for RNAscope ISH image analysis
- Workflow for quantitation of both chromogenic and fluorescent staining
- Examples of RNAscope, BaseScope, and miRNAscope QuPath analysis
Dates and Registration:
You can watch a video or download presentation of the past webinars.
- 2021 Feb 23 - ACD Support Webinar: A Deep Dive Into the RNAscope Chromogenic Assay Workflows
- 2021 Feb 19 - Webinar: From RNAscope to Exosomes
- 2021 Feb 16 - Spatial Profiling of Lung Tissue from SARS-CoV-2-Positive Rapid Autopsies
- 2021 Feb 11 - ACD PAS RNAscope ISH Services for Biomarker Assay Development
- 2021 Feb 10 - Knowledge Resource Series - Liver & Kidney Disease Applications
- 2021 Feb 4 - ACD | Rutgers Cancer Institute of New Jersey
- 2021 Feb 2 - Knowledge Resource Series - COVID-19 Applications
- 2021 Jan 28: ACD PAS Webinar: PAS Webinar: RNAscope ISH services for Gene Therapy Biodistribution
- 2021 Jan 26: ACD Support Webinar - Overview of the RNAscope Technology Based on Key Applications
- 2021 Jan 20 Knowledge Resource Webinar Series - Neurodegenerative Disease Applications
- 2021 Jan 14 Knowledge Resource Webinar Series - Oncology Applications
- 2020 Dec 17: ACD Support Webinar - Best of 2020 Applications
- 2020 Dec 15: PAS Webinar: Use of RNAscope ISH services for T-Cell Therapy Detection & Quantification
- 2020 Dec 2 Knowledge Resource Webinar Series - Applications Using BaseScope
- 2020 Dec 1: ACD Support Webinar - Tips and Tricks for Getting Started with miRNAscope™ Assay
- 2020 Nov 17 - ACD Webinar: Now Available to Academic Labs - RNAscope ISH Services for Spatial Mapping of Gene Expression
- 2020 Nov 12 - ACD Neuro Summit
- 2020 Nov 2 - Knowledge Resource Series - Single-Cell Applications
- 2020 Nov 17 - Spatial Expression Analyses Elucidates Mechanisms of circRNAs in Cancer
- 2020 Nov 20 - Companion Diagnostic and Biomarker Assay Development for Interrogation of Liquid and Solid Tumor Biopsies – From RNAscope to Exosomes
- 2020 Oct 27 - Visualize your RNA and RNA-Protein Co-Detection using RNAscope™ Multiplex Fluorescent V2 Assay
- 2020 Oct 26 - Knowledge Resource Series - Inflammation Applications
- 2020 Oct 23 - Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples
- 2020 Oct 19 - Knowledge Resource Series - Pain Medicine
- 2020 Oct 6 - Longitudinal Immune Profiling Reveals Key Immune Signatures Associated with COVID-19
- 2020 Sept 29: Introducing ACD's New RNA-Protein Co-Detection Assays
- 2020 Sept 22 - Knowledge Resource Series - Stem Cell Applications Webinar
- 2020 Sept 21 - Resolving the fibrotic niche – a single-cell RNA sequencing approach
- 2020 Sept 4 Incorporation and Automation of RNAscope Technology into your COVID-19 Viral Pathogenesis Research
- 2020 Sept 17 Webinar - Understanding Pain Mechanisms: RNA Expression Analysis of Peripheral Nervous System Tissue
- 2020 Sept 9 Webinar - Spatial Mapping and in situ Confirmation of Gene Signatures using RNAscope HiPlex Technology
- 2020 Aug 31 - Knowledge Resource Series - Spatial Genomics Applications Webinar
- 2020 Sept 15 ACD Sales Webinar - Accelerate target validation using the RNAscope ISH automated assay platform
- 2020 Sept 10 - Clinical Assay Development using ACD’s in situ Hybridization Assay Services
- 2020 Sept 2 - Spatial Profiling to Assess a Novel Gene Therapy in a Mouse Model of a Rare Genetic Disorder
- 2020 August 24 - Knowledge Resource Series - Diagnostic Applications Webinar
- 2020 Aug 6 - Spatial Mapping of Gene Expression in Tumors Using RNAscope Technology
- 2020 Aug 25 - RNAscope® Automated Assays Solutions: Leica BOND RX System and Roche Discovery Ultra.
- Characterizing Complex Tissues with Spatial Expression Analysis Using the RNAscope Technology
- 2020 Aug 12 - Solving Cell and Gene Therapy Hurdles with Innovative Tools
- 2020 July 28 - miRNAscope Launch Webinar: Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
- 2020 Aug 18 - Visualize ASO, miRNA, and siRNA Detections with Spatial Resolution using the New miRNAscope™ Assay
- 2020 July 6 - Knowledge Resource Series #4 COVID & Infectious Disease Webinar
- 2020 July 14 - Viral Detection by ACD’s in situ Hybridization Assay Services
- 2020 June 23 - Knowledge Resource Series #3 Biomarker Development Webinar
- Spatial mapping and in situ confirmation of gene signatures using RNAscope HiPlex Technology
- 2020 June 24: Understanding pain mechanisms: RNA expression analysis of peripheral nervous system tissue
- 2020 Jun 16 - Knowledge Resource Series#2 Gene Therapy Webinar
- 2020 Jun 19 - Probes: An Overview of Probe Design Technology, Application and New Probe Requests
- 2020 Jun 9 - Knowledge Resource Series#1 T-Cell Therapy Webinar
- 2020 June 10 - ACD Re-Broadcast: Viral Pathogenesis Research: Molecular Detection with RNAscope
- 2020 May 26 - Visualize Splice Variants, Short Targets, and Point Mutations at Single Cell Resolution
- 2020 May 21 - Tissue-based expression analysis by ACD’s in situ hybridization assay services — Simultaneous ISH/IHC Co-Detection of RNA and Protein Targets
- 2020 June 26 - Rebroadcast: Preview of the new RNA ISH Assay to Detect miRNAs, siRNAs and ASOs
- 2020 May - ACD Spatial Genomics Seminar Series
- 2020 May 12 - A Spatial Genomics Webinar - ACD & NanoString
- Visualize Gene Expression with Morphological and Spatial Resolution using RNAscopeTM Chromogenic Assay
- Tissue-Based RNA Detection with ACD’s Pharma Assay Services: Quantitative Image Analysis From Histopathology Experts
- 2020 Apr 14 - Resolving Cell Subtype Specialization with scRNA-seq and RNAscope
- Transcriptional and Spatial Resolution of Cell Types in the Mammalian Habenula
- Tissue-based expression analysis by ACD’s in situ hybridization assay service — high resolution image data in as little as 4 weeks
- Spatial Genomics Seminar by ACD & NanoString
- RNAscope™ Technology Introduction: How to get started as a new user
- 2020 Feb 25 Getting Started and Troubleshooting Tips for BaseScope™ Assays
- Genome-wide Study Reveals a Pseudogene-Associated Recurrent Gene Fusion in Prostate Cancer
- 2020 Jan 28: Visualize your research breakthroughs with RNAscope™ Multiplex Fluorescent assays
- 2019 Year in Review: Key Publications Utilizing the RNAscope™ Technology
- Examination of Microglia at Single Cell Resolution in Health and Disease
- Becoming an Expert: Tips & Tricks for Running the RNAscope® Assay
- RNAscope/BaseScope Probe Design Overview Webinar
- 2019 Nov 12: Single-Cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-Related Macular Degeneration
- Pancreatic Cancer Therapeutic Target Visualized Using In Situ Hybridization
- Molecularly Guided High Plex Spatial Profiling in the Tumor Microenvironment
- Validating single-cell RNA-seq data using Single-Cell Western blotting and RNAscope® technology
- Mind the Yap! Single-Cell Transcriptomes Reveal Drivers of Intestinal Regeneration
- 2019 May 14: Motor neuron size dependent mechanisms of neuroplasticity following spinal cord injury
- A Cellular and Molecular Phenotyping Pipeline for Target Characterization in FFPE Biobank Samples
- Using tools such as single cell RNAseq and spatial mapping, researchers can determine the presence and distribution of monocytes - and their possible roles - in human autoimmune disease.
- Webinar : Visualize Splice Variants, Short Targets, and Point Mutations at Single Cell Resolution
- Training Webinar : Using CellProfiler to Analyze Your RNAscope® Images
- Sensory Experience Shapes a Growing Brain: Regulation of Sensory-Dependent Circuit Refinement by TWEAK/Fn14 Cytokine Signaling
- New Product Introduction: The RNAscope® HiPlex Assay
- Spatial Mapping of Gene Expression at Single Cell Resolution with the RNAscope® Technology
- Becoming an Expert: Tips and Tricks for Getting Started with RNAscope® Assay
- Becoming an Expert: Tips and Tricks for Running BaseScopeTM Assay
- How to correctly interpret your RNAscope® images
- Tips and Tricks for Running the RNAscope® Multiplex Fluorescent Assay
- Tips and Tricks for Getting Started with the RNAscope® and BaseScope™ Assays
- Cancer Gene Expression Targets Validated Using Novel Histopathology Tools
- Role for NaV1.3 in the developing cerebral cortex
- Using single cell RNAseq and in situ hybridization to determine neuronal composition
- Tips and Tricks for Getting Started with RNAscope® and BaseScope™ Assays
- 2018 Dec 3: Application of a Novel ISH Approach to Elucidate Splice Variants in Schizophrenia
- Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
- The Basescope™ Duplex Product Launch Webinar - 10/30 9AM PT
- The RNAscope® Assay Data Analysis Webinar
- Getting Started and Troubleshooting Tips for RNAscope® and BaseScope™ Assays
- Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
- RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS
- Mind the Transcripts: Visualizing RNA Expression and Variants within the Brain
- Elucidation of stem cell dynamics via Rspondin3 regulation using multiplex RNA in situ hybridization techniques
- Understanding heterogeneity in cancer through high-resolution imaging
- RNA in situ Hybridization: The Next Generation of Immunohistochemistry?
- BaseScope™ LS and VS Automated Assays Webinar
- Visualize your research breakthroughs with RNAscope® Multiplex Fluorescent assays
- Tips for Getting Started with RNAscope® and BaseScope™ Assays
- Webinar: Studying the Genetic Basis of Neurodevelopmental Channelopathies
- Tips, Tricks & Troubleshooting RNAscope® and BaseScope™ Assays
- Genetic Variant Detection in Cancer: Using RNAscope® ISH to Track Tumor Evolution
- CAR-T Cell Target Selection Using Innovative in Situ Hybridization Technology
- Getting Started and Troubleshooting Tips for RNAscope® and BaseScope™ Assays
- Combined immunohistochemistry and RNA in situ hybridization to elucidate tumor molecular heterogeneity in prostate cancer
- Characterization of immune checkpoint expression & infiltrating immune cells in TME by RNAscope ISH
- RNAscope® Assay Data Analysis Webinar
- Tips & Tricks to Get Publication Quality RNA ISH Data for Neuroscience Applications
- Product Introduction: RNAscope® VS Universal HRP, AP and Duplex Assays
- Get the most out of your RNAscope® Multiplex Fluorescent assays
- Optimize Your RNAscope® Assay Data Analysis
- Tips, Tricks & Troubleshooting to Get Publication Quality RNA ISH Data
- RNAscope® Assay Data Analysis Webinar
- BaseScope™ Assay: Recent Applications & FAQs
- Introduction to RNAscope® Probes
- New Product Introduction: RNAscope® Multiplex Fluorescent v2 Assay
- New Product Introduction: RNAscope® Multiplex Fluorescent v2 Assay
- Introduction to the RNAscope® Assay
- Visualize your research breakthroughs with RNAscope® and BaseScope™ assays
- Steps for Success with the RNAscope® Multiplex Fluorescent Assay
- RNAscope® Assay Data Interpretation
- Tips for Getting Started with the RNAscope® Multiplex Fluorescent Manual Assay
- Elucidating tumor heterogeneity in prostate cancer by combined IHC & RNA ISH
- New product introduction - BaseScope™ Assay, a new and innovative RNA ISH platform for the detection of short targets and exon junctions.
- New product introduction - BaseScope™ Assay, a new and innovative RNA ISH platform for the detection of short targets and exon junctions.
- New Product Introduction: RNAscope® LS Multiplex Fluorescent Assay
- Tips for Getting Started with the RNAscope® Multiplex Fluorescent Manual Assay
- Advanced Cell Diagnostics’ Pharma Assay Services – Expert capabilities in RNAscope ISH assays and analysis for detection and quantification of mRNA, transcript isoforms and point mutations.
- RNA-ISH for long non-coding RNAs (lncRNAs) - novel game changers in pathology
- Steps for Success with RNAscope® 2.5 VS on the Discovery Ultra and Discovery XT Automated Assays
- 2016 June 21st: Steps for Success with RNAscope® Assays & New Product Development
- Current State-of-the-Art Tissue-Based RNA Analysis with RNAscope Technology & Quantitative Analysis with the HALO Image Analysis Platform
- 2016 May17th: Become an RNAscope® Expert: RNAscope® Data Interpretation Webinar
- 2016 April 26: RNAscope® New Product Development - RNAscope® 2.5 HD Duplex Assay
- 2016 April 26: RNAscope® New Product Development - RNAscope® 2.5 HD Duplex Assay
- 2016 March 15th: New RNAscope® LS Duplex Assay
- 2016 Feb 26th: Become an RNAscope® Expert with the RNAscope® Multiplex Fluorescent assay
- 2016 Jan 28th: PTEN mRNA detection by chromogenic RNA in situ technologies: A reliable alternative to PTEN immunohistochemistry
- Steps for Success with the RNAscope® Assay & New Product Development
- Dec 17th, 2015: Steps for Success with RNAscope® Assay and New Product Development
- 2014 Sept 11th: Whole-Mount Zebrafish Embryos - RNAscope® for High-Resolution Detection of Transcripts with Localization of Proteins
- RNAscope® Technology: A Novel RNA in situ Hybridization Research Platform
- RNAscope® New Product Development - learn about RNAscope 2.5 product line
- RNAscope® New Product Development - learn about RNAscope 2.5 product line
- Linking Viral Discovery with Causality
- Dec 17th, 2015: Steps for Success with RNAscope® Assay and New Product Development
- Visualization of exogenous RNA using RNAscope ISH in rat brain to assess viral vector transduction
- Elucidating tumor heterogeneity in prostate cancer by combined IHC & novel RNA ISH
- Steps for success with the RNAscope assay: Basic training for new users
- Steps for success with the RNAscope assay: Basic training for new users
- Steps for success with the RNAscope assay: Basic training for new users
Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.
How to use EZ-Batch Tray
De-paraffinization
De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.
Endogenous Peroxidase Blocking
RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.
Target Retrieval (Boiling)
Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.
Protease Plus (Protease Digestion)
Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.
Target Probe Hybridization
ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target
Amplification (Amp1-Amp6) with Wash Step
RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.
DAB Colormetric Reaction
Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.
Gill's Hematoxylin Counter Stain
Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe the morphology of the sample and identify the localization of the signal.
Tissue Dehydration
The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better tissue morphology and preservation of the signal.
Mounting
The final step in RNAscope after staining requires the use of mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.
How can we help you?
Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

1 (877) 576-3636