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Launch Webinar: Introducing ACD's New RNA-Protein Co-Detection Assays

Learn more about ACD’s new Co-detection Assays which will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow and reagents will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

This webcast will describe employing single cell genomics and spatial transcriptomics to discover a previously unexplored oncofetal reprogramming of the tumor ecosystem.

Analysis of ~212,000 cells representing human fetal, hepatocellular carcinoma (HCC), and mouse liver revealed remarkable fetal-like reprogramming of the tumor microenvironment. Specifically, the HCC ecosystem displayed features reminiscent of fetal development, including re-emergence of fetal-associated endothelial cells and fetal-like tumor-associated macrophages.

The speaker will describe a shared immunosuppressive oncofetal ecosystem in fetal liver and HCC and its potential implications for targeting therapeutic interventions in HCC and identifying similar paradigms in other cancers and diseases.

• Identification of fetal-associated endothelial cells and macrophages in HCC
• Shared oncofetal ecosystem between human fetal liver and HCC
• The phenomenon of oncofetal reprogramming in the tumor ecosystem

• Visualize the cellular heterogeneity of tissues and solid tumors with the multiplexing and spatial capabilities of the RNAscope Multiplex Fluorescent assay

• Specific and sensitive biomarker validation to accelerate translation of assays into the clinic

• Morphology-based biodistribution analysis of AAV vector and transgene expression

• CAR-T and TCR-T cell tumor infiltration and activity assays

• Detection of splice variants, point mutations and short target sequences with BaseScope ISH assays

• Co-stain with cell-type markers using our ISH/IHC Co-Detection workflow

• PAS’s quality-controlled tissue bank including solid tumors, diseased and normal human tissues, and TMAs

• RNAscope image analysis offerings for quantitative approaches to scoring

• Tissue preparation

• Tissue pretreatments

• RNAscope V2 assay

• Sample visualization and image analysis

• Overview of RNAscope technology and general imaging guidelines

• Introduction of QuPath for RNAscope ISH image analysis

• Workflow for quantitation of both chromogenic and fluorescent staining

• Examples of RNAscope, BaseScope, and miRNAscope QuPath analysis