• May
    08
    2024 May 8 - Expand your Multiomic Capabilities with RNAscope™
    Blake McAlpin, Ph.D.,, Field Applications Scientist, Advanced Cell Diagnostics, Bio-Techne.
    Paul Liu, Ph.D.,, Sr. Product Manager, Advanced Cell Diagnostics, Bio-Techne
    2024 May 8 - Expand your Multiomic Capabilities with RNAscope™
    Blake McAlpin, Ph.D.,
    Field Applications Scientist, Advanced Cell Diagnostics, Bio-Techne.
    Paul Liu, Ph.D.,
    Sr. Product Manager, Advanced Cell Diagnostics, Bio-Techne
    Outline:
     
     

    In this webinar we will provide an overview of RNAscope™ ISH technology, then discuss our new RNAscope multiomic applications coming soon. RNAscope is the gold standard for RNA ISH -providing single-molecule sensitivity and unmatched specificity at subcellular resolution. Backed by over 10,000 peer reviewed publications, RNAscope has enabled researchers to reveal ground-breaking biologic discoveries, accelerate the development of next generation therapeutics, and deliver life-changing diagnostics.

     

    Learn about the extensive menu of RNAscope assays available for use with the BOND RX that will enable you to detect a wide range of different RNA biomarkers in any species or tissue. Easily incorporate RNAscope in your program to screen biomarkers from discovery programs, visualize secreted biomarkers including cytokines or chemokines, detect low expressing biomarkers, or utilize when an antibody is not available.

     

    Be first to see what’s coming next. Hear how you can easily perform true same-slide RNAscope multiomics using our new protease-free assay workflow that delivers optimized staining performance, preserves tissue morphology, and simplifies assay design. Enabling you to use any IHC qualified antibody for codetection of protein and RNA on the same FFPE tissue section with either chromogenic or fluorescent detection, you can easily visualize changes in cell phenotypes and function across a wide range of tissue samples. With capability to easily detect up to 6 fluorescent proteins and/or RNAs simultaneously, possibilities are unlimited.

    Learning Objectives:
    • Identify RNAscope technologies and examples of RNAscope co-detection results
    • Review the Integrated Co-Detection Workflow (ICW)
    • Outline guidelines on sample preparation and pre-treatment, including guidance on assay setup and appropriate controls
    Dates and Registration:
    May
    08
    8am PST
    11am EST
    5pm CEST
    Register Now

You can watch a video or download presentation of the past webinars.

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Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

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