Customer updates during COVID pandemic Click Here >

  • Jul
    27
    2021 July 27: Introducing the RNAscope HiPlex v2.0 assay for single-cell and spatial transcriptomic profiling.
    Anushka Dikshit, Ph.D., Applications Scientist, Genomics, Advanced Cell Diagnostics
    Purvi Shah, Sr. Product Manager, Genomics, Advanced Cell Diagnostics
    2021 July 27: Introducing the RNAscope HiPlex v2.0 assay for single-cell and spatial transcriptomic profiling.
    Anushka Dikshit, Ph.D.
    Applications Scientist, Genomics, Advanced Cell Diagnostics
    Purvi Shah
    Sr. Product Manager, Genomics, Advanced Cell Diagnostics
    Outline:

    Introducing the RNAscope™ HiPlex v2.0 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples.

    The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex v2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex v2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME)

    Learning Objectives:
    • Learn about the expanded capabilities and applications of the HiPlex v2 assay
    • Understand how the assay enables characterization of immune cell types and activation states
    • Study complex spatial relationships among different cell types within the TME
    Dates and Registration:
    July
    27
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Aug
    05
    2021 Aug 5 - PAS Webinar: RNAscope™ ISH Services for Gene Therapy Spatial Biodistribution Assessment
    Ruby Hsu, Ph.D.,, Director, Business Development, Advanced Cell Diagnostics
    2021 Aug 5 - PAS Webinar: RNAscope™ ISH Services for Gene Therapy Spatial Biodistribution Assessment
    Ruby Hsu, Ph.D.,
    Director, Business Development, Advanced Cell Diagnostics
    Outline:

    RNAscope™ in situ Hybridization Services for Gene Therapy Spatial Biodistribution Assessment

    Visual detection and quantification of AAV, lentiviral, and oncolytic viral vector genome biodistribution and transgene expression in tissues with RNAscope and BaseScope™ in situ hybridization. RNAscope in situ hybridization (ISH) has been published as a means of “morphology-based AAV biodistribution analysis.” 

    RNAscope in situ assays of vector genome DNA and transgene mRNA combine the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology. Multiplexing enables vector genome and transgene mRNA to be visually assessed and quantified simultaneously in a single slide. With the addition of cell marker probes in multiplex fluorescent RNAscope assays, viral tropism can be measured in any cell population. 
    Specific measurement of therapeutic human transgene expression in animal models is often impossible by IHC because of the high levels of homology between transgene and animal ortholog transcripts. BaseScope ISH is another powerful tool that enables specific detection of even single nucleotide differences and can easily differentiate human transgenes from non-human primate and other animal ortholog sequences. 
    These assays are enabling more informative and efficient preclinical gene therapy programs and contributing critical data in IND-enabling studies. Please join us to hear how our services are contributing to and advancing gene therapy programs worldwide.

    Learning Objectives:
    Key features of RNAscope gene therapy assays:
    • Monitor AAV vector biodistribution and transgene mRNA expression in conventional FFPE tissues
    • Quantitative, single-cell measurement of vector genome and transgene multiplexed with cell-type markers
    • Use off-the-shelf probes for WPRE or promoter sequences, or design custom probes in 2 weeks
    Dates and Registration:
    August
    05
    10am PST
    1pm EST
    7pm CEST
    Register Now
  • Aug
    25
    2021 Aug 25 - Mapping Neuronal Gene Expression to Understand Pain
    Ted Price, PhD, Eugene McDermott Professor Director, Center for Advanced Pain Studies University of Texas at Dallas
    Diana Tavares-Ferreira, PhD, Postdoctoral Fellow Center for Advanced Pain Studies University of Texas at Dallas
    Stephanie Shiers, PhD, Postdoctoral Fellow Center for Advanced Pain Studies University of Texas at Dallas
    2021 Aug 25 - Mapping Neuronal Gene Expression to Understand Pain
    Ted Price, PhD
    Eugene McDermott Professor Director, Center for Advanced Pain Studies University of Texas at Dallas
    Diana Tavares-Ferreira, PhD
    Postdoctoral Fellow Center for Advanced Pain Studies University of Texas at Dallas
    Stephanie Shiers, PhD
    Postdoctoral Fellow Center for Advanced Pain Studies University of Texas at Dallas
    Outline:

    The human dorsal root ganglion (DRG) is the site of many sensory neurons, including pain-sensing neurons called nociceptors. Therefore, studying the DRG is important for understanding pain responses—especially chronic pain development. Researchers can characterize the DRG by mapping gene expression in its cells. In this webinar brought to you by ACD – a BioTechne brand, Ted Price, Diana Tavares-Ferreira, and Stephanie Shiers will discuss mapping the DRG to identify different neuronal subtypes, thereby aiding target identification and drug discovery with the ultimate goal of developing non-opioid painkillers.

    Learning Objectives:
    • Transcriptional differences in sensory neuron subtypes in the human DRG
    • How differences in neuronal gene expression between species affect drug target development
    • How researchers go from target identification to drug development
    Dates and Registration:
    August
    25
    11:30am PST
    2:30pm EST
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

How to use EZ-Batch Tray

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

Contact ACD Technical Support

How can we help you?

Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Bio-Techne

19 Barton Lane  
Abingdon Science Park
Abingdon
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420

 

 

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china.ACD@bio-techne.com
For general information: Leon.Li@bio-techne.com
Web: www.acdbio.com/cn

For general information: Info.ACD@bio-techne.com
For place an order: order.ACD@bio-techne.com
For product support: support.ACD@bio-techne.com
For career opportunities: hr.ACD@bio-techne.com