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Age-related macular degeneration (AMD) is one of the leading causes of blindness in the elderly. Although genome-wide association studies have identified genetic variants associated with AMD, it has been challenging to identify the cell types driving this genetically complex disease. We performed single-cell RNA sequencing (scRNA-seq) of normal human retinas, and report the first single-cell human retinal transcriptomic atlas identifying all major cell types. Gene-expression heterogeneity was observed in Müller glial cells, suggesting that human retinal glia are more diverse than previously thought. The activity of genes associated with AMD was significantly enriched in vascular and glial cells, as well as in subpopulations of neurons. Furthermore, AMD disease-associated gene activity was consistent with regional macular disease susceptibility. Findings were validated using in situ hybridization techniques and immunofluorescence. Our data show how scRNA-seq can provide insight into relevant cell types for a complex genetic disease. 

• Single-cell transcriptomic atlas of the human retina
• Identification of cell types associated with AMD
• Validation of findings using RNAscope^®

RNAscope/BaseScope Probe Design Overview Webinar

Outline: Taking advantage of the RNAscope/BaseScope probe design strategies to achieve highly specific and sensitive detection of a variety of RNA targets in situ, including but not limited to RNA, mRNA, pre-RNA, TCR/BCR, KO validation, splice variant, circRNA, and fusion transcript.

Who should attend:

• Researchers interested in an overview of RNAscope/BaseScope probe design strategies.
• Researchers interested in knowing the scope of targets that can be specifically detected by RNAscope/BaseScope probes.
• Researchers interested in submitting standard probe design requests through ACD on-line portal.
• Researchers interested in submitting non-standard probe design requests through ACD on-line portal.

Learning Objectives:

• Introduction to the RNAscope/BaseScope design workflow.
• Featured applications of RNAscope/BaseScope probes including detection of mRNA, pre-RNA, KO event, TCR/BCR clone, splice variant, circRNA, gene fusion
• Frequently asked questions - Applications (FAQs)
• Step-by-step instructions and tips on filling the on-line new probe request (NPR) form.