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In this webinar, you will hear from Dr. Lique Coolen and Dr. Aleisha Moore from Kent State University on how they have integrated powerful RNAscope in situ hybridization technology in their research to study the mechanisms underlying spinal cord injury and neuroendocrine dysfunction.



Dr. Lique Coolen will provide an overview of studies in her laboratory that examine the impact of spinal cord injury on the spinal reflex generator for sexual function in the lumbar spinal cord in male rats. Recent evidence of injury-induced changes in transcript levels of neuropeptides and identification of localization of receptors in this spinal generator using RNAscope in situ hybridization will be presented. Furthermore, pharmacological experiments towards treatment development will be introduced.



Dr. Aleisha Moore will present work in her laboratory identifying changes in neuronal circuits controlling fertility that may lead to polycystic ovarian syndrome, the most common cause of infertility in women worldwide. In her talk, Dr. Moore will focus on how she used RNAscope HiPlex technology in a mouse model of PCOS to evaluate changes in gene expression patterns within complicated and heterogenous hypothalamic neuronal circuits that may underlie the pathogenesis of PCOS.

Key learning objectives in Dr. Coolens talk:

• Learn about impacts of spinal cord injury on urogenital function.
• Become familiar with the use of RNAscope to identify G-protein coupled receptors in spinal cord.

Key learning objectives in Dr. Moore’s talk:

• Learn about the neuroendocrine pathogenesis of PCOS.
• Learn about the current use of RNAscope HiPlex technology to study hypothalamic cell populations.
• Become familiar with Cellprofiler software for automated analysis of RNAscope HiPlex data.

This webinar will present a research project that is using spatial transcriptomics to discern the molecular mechanisms underlying fear memory. 

Kaitlin Sullivan of the University of British Columbia will discuss how she is using multiplexed, fluorescent RNA in situ hybridization (mFISH) based on the RNAscope technology to assess neuronal activity during the creation and recollection of fear memory.

• The use of mFISH to spatially resolve transcriptomically unique neuronal subpopulations.
• How to computationally analyze images and quantify expression at single-cell, single-molecule resolution.
• How to pair mFISH with morphological, circuit, and functional information to get a holistic view of cell types across multiple spatial scales.

• Introduction to the RNAscope/BaseScope/miRNAscope/DNAscope design workflow. 
• Featured applications of RNAscope/BaseScope/miRNAscope/DNAscope probes including detection of RNA, mRNA, pre-RNA, mature microRNA, ASO, siRNA, TCR/BCR, Knockout validation, splice variants, DNA copy number and structural variations. 
• Frequently asked questions - Applications (FAQs).

• Introduction to the BaseScope technology and workflow
• Visualizing specific CDR3 sequences on T cells in tumor samples
• Simultaneous detection of two variants of the same gene
• Detecting circular RNA and other splice variants implicated in neurological diseases
• • Detecting point mutations in sub-clonal populations within heterogenous tumors

The RNAscope™ technology enables fast and rigorous target and biomarker development: it is a robust and ultra-sensitive RNA in situ hybridization (RNA ISH) method providing single-cell and single-molecule RNA detection in the context of complex tissue microenvironments. Advanced Cell Diagnostics’ Professional Assay Services is the only CRO specialized in providing RNAscope and BaseScope ISH.  

In this webinar, we will introduce assay service offerings that combine analysis of any target by RNAscope in situ hybridization (ISH) with immune cell and checkpoint marker immunohistochemistry (IHC) and immunofluorescence (IF) assays. Our services provide spatial analysis of target antigens, biomarkers, cytokines, or CAR-T cells together with immune cell distribution within the tumor microenvironment (TME). All RNAscope probes can be multiplexed together with our validated IHC and IF assays. Please join us to hear how our services are contributing to and advancing the therapeutic development programs of hundreds of pharma and biotech companies worldwide.

• Use of ISH-IHC/IF assays to support preclinical immuno-oncology research and biomarker validation
• Surveillance of immune cell and check-point markers in the TME
• CAR-T and TCR T-cell tumor infiltration and activation in pre- vs post-treatment clinical biopsies
• Validated ISH/IHC protocols for co-detection of biomarkers and immune cell markers
• Quality-controlled tissue bank that includes solid tumors, normal human tissues, and TMAs available for target safety/ADME assessment or any other research needs