The tumor microenvironment is a complex network of different cell types and states that imparts distinct properties to the tumor and plays a significant role in determining the efficacy of cancer therapies. Immunotherapies in particular have demonstrated considerable therapeutic efficacy and durable response for several tumor types, however the majority of patients are resistant or relapse after initial response. More precise spatial characterization of the tumor and tumor microenvironment with high multiplexing can potentially provide critical insight into new immunotherapeutic strategies and identify new predictive biomarkers for stratifying patients most likely to benefit from immunotherapy.
Using the RNAscope™ in situ hybridization (ISH) technology from Advanced Cell Diagnostics, Inc. to molecularly guide Nanostring’s GeoMx™ Digital Spatial Profiler (DSP), it is now possible to characterize the tumor and the tumor microenvironment using 1,500+ RNA probes. The single molecule and single cell capabilities of the RNAscope ISH assay allows researchers to select discreate regions of interest for high plex spatial analyses of RNA with the highly multiplexed spatial profiling capabilities of GeoMx DSP.
In this webinar we will demonstrate how RNAscope ISH assays can be combined with GeoMx DSP to assess expression of RNA from a specific regions of interest and perform follow up confirmation of target gene expression at the single-cell level, all with spatial context.
Key learning objectives:
- Introduction to GeoMx DSP and RNAscope ISH technologies
- Learn detailed workflow for molecularly guided high plex spatial analysis
- Learn Compatibility and correlation between GeoMx DSP and RNAscope ISH
- Confirmation of GeoMx DSP results with RNAscope ISH at the single-cell level
