The RNAscope and BaseScope Assays are based on the proprietary RNAscope Technology enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity. Learn more about the difference between RNAscope, BaseScope and miRNAscope Assays.
Using proprietary ACD RNAscope Probe Design Pipeline, we provide double "Z" oligo probes designed to hybridize to your specific RNA target of interest. We can design in situ hybridization RNA probes for virtually ANY gene in ANY genome for visualization in ANY tissue or Cells.
Previously designed and used by our customers, they can be shipped to you in approx. 2 weeks. Our catalog probes span over 40,000 RNA targets in over 400 species including human, mouse, rat, dog, cow, zebrafish, rabbit, pig, chicken, monkeys and over 100 viruses. Search and select from our over ever-growing catalog of in situ hybridization probe sets.
New probes for your gene of interest will be designed and manufactured in about 2 weeks.
Under certain conditions, ACD may agree to grant exclusivity to probes against "proprietary and private" targets for a fee. While ACD still owns the probe design for these targets, we are committed to not market these probes until the probe exclusivity is removed. Please talk to your account executive for further information.
- ProbeProbe Type
- HsSpecies
- FDXRGene
- 5UTRSpecification
- C2Amplification Channel
This probe used in Leica Automated System Assay(LS Probe) and is designed to hybridize to Mus musculus (Mm) Rspo3 gene. Its spectral channel is not listed which means Spectral Channel 1 and therefore compatible with any of the four manual assays including RNAscope 2.5 HD Reagent Kit-BROWN, RNAscope 2.5 HD Reagent Kit-RED, RNAscope 2.5 HD Duplex Reagent Kit or RNAscope Multiplex Fluorescent Reagent Kit.
This is probe is designed to be used with a manual assay (Probe). It targets just the 5' untranslated region (5UTR) of the GNRHR gene from the human sequence(Hs). This probe is compatible with spectral channel 2 (C2), so it can be used in a duplex or multiplex assay.
- BAPlatform
- MmSpecies
- Nrg1Gene
- E1E2Sub-Type
This probe is compatible with Manual BaseScope assays and is designed to hybridize to Drosophila melanogaster Gapdh1 gene. The 3ZZ specifies the number of probe pairs and indicates this is a short target probe.
Probes | RNAscope Manual Assays | RNAscope 2.5 LS (Leica Biosystems) | RNAscope 2.5 VS (Roche Tissue Diagnostics Systems) | BaseScope Manual Assay | BaseScope LS (Leica Biosystems) | BaseScope VS (Roche Tissue Diagnostics Systems) | miRNAscope Manual Assay | miRNAscope LS (Leica Biosystems) |
|---|---|---|---|---|---|---|---|---|
Probe Name & Naming convention | Diaminobenzidine | 2.5 LS RNAscope Probe | 2.5 VS RNAscope Probe | BaseScope Probe | BaseScope LS Probe | BaseScope VS Probe | miRNAscope Probe | miRNAscope LS Probe |
Probe naming convention | Probe Type-Species-Gene Name-Channel | Probe Type-Species-Gene Name | Probe Type-Species-Gene Name | Platform-Species-Gene-Sub-Type | Platform-Species-Gene-Sub-Type | Platform-Species-Gene-Sub-Type | Platform-Species Type-Gene-Strand-Amplification Channel | Platform-Species Type-Gene-Strand-Amplification Channel |
Spectral Channel Compatibility | Available for Channel 1-4 | Available for Channel 1-4 | Available for Channel 1 and 2 Only. | Channel 1 and 2 Only. When Channel No. is missing, the probe is a C1 probe. | Channel 1 Only. When Channel No. is missing, the probe is a C1 probe. | Channel 1 Only. When Channel No. is missing, the probe is a C1 probe. | Channel S1 Only. | Channel S1 Only. |
C1 Probes Assay Compatibility | C1 is spectral channel 1 and is the most abundant probe type. RNAscope 2.5 HD Reagent Kit--RED RNAscope 2.5 HD Duplex Reagent Kit RNAscope Fluorescent Multiplex Reagent Kit RNAscope Multiplex Fluorescent Reagent Kit v2 RNAscope HiPlex12 Reagents Kit (488, 550, 647) RNAscope HiPlex12 Reagents Kit (488, 550, 647, 750) | RNAscope 2.5 LS Assay Reagent Kit--BROWN RNAscope 2.5 LS Assay Reagent Kit--RED RNAscope 2.5 LS Duplex Reagent Kit RNAscope LS Multiplex Fluorescent Reagent Kit | RNAscope VS Universal HRP Reagent Kit RNAscope VS Universal AP Reagent Kit RNAscope VS Duplex Reagent Kit | BaseScope Reagent Kit v2–RED BaseScope Duplex Reagent Kit | BaseScope LS Reagent Kit | BaseScope VS Reagent Kit | Channel S1 miRNAscope HD Reagent Kit - RED | Channel S1 miRNAscope LS Reagent Kit - RED |
C2 Probes Assay Compatibility | C2 is spectral channel 2 RNAscope Fluorescent Multiplex Reagent Kit RNAscope Multiplex Fluorescent Reagent Kit v2 | RNAscope 2.5 LS Duplex Reagent Kit RNAscope LS Multiplex Fluorescent Reagent Kit | RNAscope VS Duplex Reagent Kit | BaseScope Duplex Reagent Kit | Not Available | Not Available | Not Available | Not Available |
C3 Probes Assay Compatibility | C3 is spectral channel 3 RNAscope Multiplex Fluorescent Reagent Kit v2 | RNAscope LS Multiplex Fluorescent Reagent Kit | Not Available | Not Available | Not Available | Not Available | Not Available | Not Available |
C4 Probes Assay Compatibility | C4 is spectral channel 4 | RNAscope LS 4-Plex Ancillary Kit for LS Multiplex Fluorescent | Not Available | Not Available | Not Available | Not Available | Not Available | Not Available |
T1-T12 Probes Assay Compatibility | HiPlex12 Reagents Kit (488, 550, 647) | Not Available | Not Available | Not Available | Not Available | Not Available | Not Available | Not Available |
Probe Volume | Probes for 20 slides (C1-C4) | Probes for 30 slides | Probes for 30 slides | Probes for 20 slides | Probes for 30 slides | Probes for 30 slides | Probes for 20 slides | Probes for 30 slides |
Species Designation Probe name contains two letters to indicate species name | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien | Hs-Homo Sapien | Hsa-Homo Sapien | Hsa-Homo Sapien |
